A high-efficiency lignocellulose-degrading bacteria k24 and its application

A lignocellulose-based, high-efficiency degrading bacteria technology, applied in bacteria, microorganisms, microorganism-based methods, etc., can solve the problem of low enzyme activity, and achieve stable enzyme activity, rapid value-added, significant lignin degradation ability and hemicellulose. The effect of degrading ability

Active Publication Date: 2018-10-16
黑龙江省农业科学院牡丹江分院
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Problems solved by technology

However, the use of fungi to degrade lignocellulose generally has the problem of low enzyme activity

Method used

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  • A high-efficiency lignocellulose-degrading bacteria k24 and its application
  • A high-efficiency lignocellulose-degrading bacteria k24 and its application
  • A high-efficiency lignocellulose-degrading bacteria k24 and its application

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specific Embodiment approach 1

[0024] Specific embodiment one: a lignocellulosic material efficient degrading bacterium K24 of this embodiment, it is enterobacter hormaechei (Enterobacter hormaechei) K24, is preserved in the common microorganism center of China Committee for Microorganism Culture Collection, and the preservation address is Beijing No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, the city, the date of preservation is December 12, 2014, and the preservation number is CGMCC No.10169.

[0025] Enterobacter hormaechei (Enterobacter hormaechei) K24 of the present embodiment is a Gram-negative bacterium, and the bacterial cell shape of this strain is rod-shaped, and the bacterial cell size is 0.52~0.56×1.3~1.5 μm, does not form spores, and has no flagella and pods Membrane; forms round, opaque, milky-white colonies with smooth protrusions and neat edges on LB medium (such as figure 1 shown).

[0026] The results of the physiological and biochemical reactions of the strain included 18...

specific Embodiment approach 2

[0087] Embodiment 2: The application of a lignocellulosic substance-degrading bacteria in this embodiment, which is used to degrade lignocellulosic substances.

[0088] Bacterial strain of the present invention is carried out following functional detection:

[0089] Enterobacter hormaechei (Enterobacter hormaechei) K24 of the specific embodiment was tested for weight loss rate and lignin, cellulose and hemicellulose degradation of bagged fungus waste and corn stalks to verify its unique functions. details as follows:

[0090] 1. Determination of weight loss rate of bagged fungus waste and corn stalks

[0091] 1.1 Corn stalk powder

[0092] Corn stalks were taken from the experimental field of the laboratory in Hulan Campus, Heilongjiang University, Harbin City, Heilongjiang Province in October 2012, dried, crushed and passed through a 40-mesh sieve, and set aside.

[0093] 1.2 bags of fungus waste

[0094] Bags of fungus waste were provided by Mudanjiang Branch of Heilongj...

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Abstract

The invention relates to a lignocellulose substance efficient degradation bacterium K24 and an application thereof. The lignocellulose substance efficient degradation bacterium K24 is enterobacter hormaechei K24 and is collected in the China General Microbiological Culture Collection Center (CGMCC) on December 12, 2014, with collection number of CGMCC No.10169. The lignocellulose substance efficient degradation bacterium K24 is used for degrading lignocellulose substances. The lignocellulose substance efficient degradation bacterium K24 has the beneficial effects that the strain is rapid in proliferation, has strong adaptability and is widely applied; the weight loss ratios of waste agaric material substitutes subjected to fermentation treatment with enterobacter hormaechei K24 are more than 39%; and the weight loss ratios of corn straws subjected to fermentation treatment with enterobacter hormaechei K24 are more than 29%.

Description

technical field [0001] The invention relates to a high-efficiency degrading bacterium K24 of lignocellulosic substances and its application. Background technique [0002] my country is a large agricultural country, and the amount of agricultural waste generated is extremely huge. Due to the limitations of economic benefits and technology popularization, most of the agricultural waste is extensively and inefficiently used and the state of idleness is serious, resulting in waste of resources and environmental pollution. Waste has become China's largest source of pollution. However, there is a large amount of lignocellulose in agricultural waste, which needs to be developed and utilized urgently. [0003] Lignocellulose is the second most abundant organic renewable resource after cellulose in nature, and it is also one of the most difficult components for microorganisms to degrade. In recent years, some lignin-degrading fungi have begun to be applied in practice, but further d...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/01
Inventor 王延锋柴永山董清山史磊董雪梅王金贺刘姿彤倪淑君黄文潘春磊盛春鸽张鹏于海洋孙靖轩刘莹张海峰赵鹤冯锡君王佰成孟祥海
Owner 黑龙江省农业科学院牡丹江分院
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