Induction medium for nauclea officinalis aseptic seedlings and nauclea officinalis detoxification rapid propagation method

A technology for inducing culture medium and sterile seedlings, which is applied to the induction medium of aseptic seedlings of Galleria japonica and the field of detoxification and fast propagation of Galleria japonica, can solve the problems of different seed maturity, long emergence cycle, low emergence rate and the like, To achieve the effect of not growing and breeding cycle, solving long breeding cycle and short breeding time

Active Publication Date: 2016-02-03
HAINAN SENQI PHARMA CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As gallwood is an important medical raw material, wild resources are severely damaged, and collection of wild resources can no longer meet production needs. Carrying out standardized production is an effective way to solve the shortage of medicinal materials, and seedling breeding is an important part of standardized production. The maturity period is long, the maturity of seeds is different, and the seeds are small, the seed coat is hard, and the dormancy period is long, which leads to a long breeding cycle of bile tree seedlings and a tight supply of seedlings, which is far from meeting the needs of the market. The popularization and cultivation and resource utilization in our country are greatly restricted
[0003] Utilizing plant tissue culture technology to establish the detoxification and rapid propagation system of P. biloba is an eff

Method used

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  • Induction medium for nauclea officinalis aseptic seedlings and nauclea officinalis detoxification rapid propagation method
  • Induction medium for nauclea officinalis aseptic seedlings and nauclea officinalis detoxification rapid propagation method
  • Induction medium for nauclea officinalis aseptic seedlings and nauclea officinalis detoxification rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Optimizing the disinfection procedure of the explant

[0031] In this experiment, the bile wood harvested in Danzhou, Wuzhishan, Baisha, Haikou and other cities and counties in Hainan Province from April to July in 2014 ( Nauclea officinalis Pierre) fruit as the experimental material.

[0032] Divide the fruits into 6 groups, disinfect them with 75% alcohol for 30-60 seconds, then sterilize them with mercuric chloride (0.1% mass concentration, containing 1% Tween) for 7-15 minutes, rinse them with sterile water 5 times until pH = 7, repeat 3 times, placed in a constant temperature incubator at 30°C under full light for cultivation, and the contamination rate and proliferation rate were counted after 10 to 12 days.

[0033] The experiments are shown in Table 1. (1) Control the disinfection time of mercuric chloride to 10 minutes, and investigate the influence of 75% alcohol disinfection time on the disinfection effect. The effect of disinfection on the fru...

Embodiment 2

[0036] Embodiment 2 medium screening and optimization

[0037] All culture mediums in this embodiment all contain the sucrose of 3.0% mass concentration; Used 3 / 4MS culture medium, 1 / 2MS culture medium all are to reduce the concentration of macroelement to 1 / 2, 3 / 4, and other components keep The resulting culture medium was unchanged.

[0038] The seeds were peeled off from the sterilized fruit of P. japonica, and the sterilized P. chinensis seeds were respectively inoculated in MS medium, 3 / 4MS medium, 1 / 2MS medium, modified medium 1 (MMS 1 , 3 / 4MS medium + 10% coconut water), modified medium 2 (MMS 2 , 3 / 4MS medium + 10% carrot puree), modified medium 3 (MMS 3 , 3 / 4MS medium + 10% mashed potato), modified medium 4 (MMS 4 , 3 / 4MS medium + 10% coconut water + 0.05mg / L DA-6), modified medium 5 (MMS 5 , 3 / 4MS medium + 10% coconut water + 0.10mg / LDA-6), modified medium 6 (MMS 6 , 3 / 4MS medium + 10% coconut water + 0.2mg / LDA-6), and then in the fully automatic light incubat...

Embodiment 3

[0041] The optimization of embodiment 3 sterile seedling induction medium

[0042] MMS described in Example 2 5 On the basis of adding GA 3 , and set 6 concentrations of 0.05mg / L, 0.10mg / L, 0.20mg / L, 0.50mg / L, 0.70mg / L and 1.00mg / L to optimize the sterile vaccine induction medium, and the control culture conditions are: The temperature is 25-28°C, the humidity is 70%-80%, the light intensity is 80-100lx, and the light is 8-10h / d, and the growth status of the sterile buds is observed.

[0043]

[0044] The results are shown in Table 3, when the control GA 3 When the concentration is 0.05 ~ 0.50mg / L, it can effectively shorten the emergence time of gallwood and increase the emergence rate, especially the effect of 0.20 and 0.50mg / L is the most obvious, which shortens the emergence time to 30d±2d and increases the emergence rate. to more than 75%, but when GA 3 When the concentration is increased again, the emergence time and emergence rate are reduced.

[0045] Therefo...

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Abstract

The invention belongs to the technical field of tissue culture rapid propagation and discloses an induction medium for nauclea officinalis aseptic seedlings and a nauclea officinalis detoxification rapid propagation method. The induction medium is prepared from a 3/4 MS medium which is prepared from 0.1%-10.0% of organic additives and 0.05-0.2 mg/L DA-6. The nauclea officinalis detoxification rapid propagation method is established by optimizing a detoxification method and optimizing aseptic seedling culture, enrichment culture and rooting culture procedures. Compared with a traditional propagation method, the advantages of being capable of saving labor, time and land, low in cost, high in propagation rate (the growth coefficient>30 generations/year), high in emergence rate, short in propagation time and high in survival rate (>95%) are achieved, the purposes of rapid and efficient propagation and large-scale popularization planting of nauclea officinalis are achieved, and a foundation is laid for industrial production of nauclea officinalis seedlings.

Description

technical field [0001] The invention relates to the technical field of rapid propagation through tissue culture, and more specifically relates to an induction medium for aseptic seedlings of B. birch and a method for detoxification and rapid propagation of B. biloba. Background technique [0002] Gallwood ( Nauclea officinalis Pierre ) is a plant of the genus Ebony in Rubiaceae. It is used as medicine with its branches, trunks, bark and other parts. It has the effects of clearing away heat and detoxifying, reducing swelling and relieving pain. , which is widely used in the treatment of mastitis, acute tonsillitis, pharyngitis, bronchitis, enteritis, cholecystitis, pneumonia, and urinary tract infections. As gallwood is an important medical raw material, wild resources are severely damaged, and collection of wild resources can no longer meet production needs. Carrying out standardized production is an effective way to solve the shortage of medicinal materials, and seedling b...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 曾纪锴
Owner HAINAN SENQI PHARMA CO LTD
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