Induction medium for nauclea officinalis aseptic seedlings and nauclea officinalis detoxification rapid propagation method

Abstract

The invention belongs to the technical field of tissue culture rapid propagation and discloses an induction medium for nauclea officinalis aseptic seedlings and a nauclea officinalis detoxification rapid propagation method. The induction medium is prepared from a 3 / 4 MS medium which is prepared from 0.1%-10.0% of organic additives and 0.05-0.2 mg / L DA-6. The nauclea officinalis detoxification rapid propagation method is established by optimizing a detoxification method and optimizing aseptic seedling culture, enrichment culture and rooting culture procedures. Compared with a traditional propagation method, the advantages of being capable of saving labor, time and land, low in cost, high in propagation rate (the growth coefficient>30 generations / year), high in emergence rate, short in propagation time and high in survival rate (>95%) are achieved, the purposes of rapid and efficient propagation and large-scale popularization planting of nauclea officinalis are achieved, and a foundation is laid for industrial production of nauclea officinalis seedlings.

Description

technical field [0001] The invention relates to the technical field of rapid propagation through tissue culture, and more specifically relates to an induction medium for aseptic seedlings of B. birch and a method for detoxification and rapid propagation of B. biloba. Background technique [0002] Gallwood ( Nauclea officinalis Pierre ) is a plant of the genus Ebony in Rubiaceae. It is used as medicine with its branches, trunks, bark and other parts. It has the effects of clearing away heat and detoxifying, reducing swelling and relieving pain. , which is widely used in the treatment of mastitis, acute tonsillitis, pharyngitis, bronchitis, enteritis, cholecystitis, pneumonia, and urinary tract infections. As gallwood is an important medical raw material, wild resources are severely damaged, and collection of wild resources can no longer meet production needs. Carrying out standardized production is an effective way to solve the shortage of medicinal materials, and seedling b...

AI Technical Summary

Problems solved by technology

As gallwood is an important medical raw material, wild resources are severely damaged, and collection of wild resources can no longer meet production needs. Carrying out standardized production is an effective way to solve the shortage of medicinal materials, and seedling breeding is an important part of standardized production. The maturity period is long, the maturity of seeds is different, and the seeds are small, the seed coat is hard, and the dormancy period is long, which leads to a long breeding cycle of bile tree seedlings and a tight supply of seedlings, which is far from meeting the needs of the market. The popularization and cultivation and resource utilization in our country are greatly restricted

[0003] Utilizing plant tissue culture technology to establish the detoxification and rapid propagation system of P. biloba is an effective way to produce high-quality high-quality seedlings of P. p. The method of tissue culture of birch tree mainly includes steps such as disinfection of explants, induction of clustered seedlings, rooting and transplanting of hardened seedlings. Technically, it all belongs to the virus-free and rapid propagation system of bile tree tissue culture, but the multiplication in the system The medium is composed of 6-BA and NAA, which cannot effectively improve the problems of long emergence period and low emergence rate in the breeding of aseptic seedlings of Panax chinensis. Due to the lack of organic substances such as coconut milk and high-efficiency auxins such as TDZ in the proliferation culture , and cannot overcome the problems of low reproductive efficiency and limited number of annual breeding seedlings in the tissue culture of B.

Images