Preparation method and application of Enteromorpha prolifera polysaccharide with immunity enhancing function

Abstract

The invention provides a preparation method and the application of Enteromorpha prolifera polysaccharide with an immunity enhancing function. The preparation method of the Enteromorpha prolifera polysaccharide comprises the steps of crude Enteromorpha prolifera polysaccharide extraction, crude Enteromorpha prolifera polysaccharide deproteinization, micromolecular impurity removing through a high-speed centrifugal method, pigment removing through an oxidation method, polysaccharide purification through a Sephacryl-300 gel chromatographic column and the like. The invention provides the simple preparation method of the Enteromorpha prolifera polysaccharide having the characteristics of stable performance, low cost, high medicinal value, capacity of increasing the immunity, and the like. Particularly, the Enteromorpha prolifera polysaccharide and an extraction method of the Enteromorpha prolifera polysaccharide are obtained by adopting the preparation method of extraction and purification as well as in-vitro immunocompetence screening and immunosuppression animal experiments, the effect of the Enteromorpha prolifera polysaccharide which is capable of enhancing the immunity function of immunosuppressed mice is clarified, and a new method is provided for effectively developing the medicinal value and the purposes of Enteromorpha prolifera, managing the environment pollution, reducing the cleaning cost and increasing the social and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of biological products, and in particular relates to a preparation method and application of enteromorpha polysaccharide with enhanced immune function. Background technique [0002] Enteromorphaprolifera is a large-scale medicinal and edible algae plant, which belongs to the Chlorophyta Ulvaceae Ulvaceae. It is widely distributed in the world, and it is mainly distributed in the southeast coastal areas of my country. The growth and reproduction performance of this algae is very strong, and its growth requires a lot of space, so that other light-demanding algae organisms in the ocean cannot survive, resulting in the death of a large number of algae; the death of enteromorpha plants can cause eutrophication of the water body, and its degradation The process can consume a large amount of oxygen, resulting in a rapid decline in dissolved oxygen in the water body, and fish suffocate and die due to lack of oxygen;...

AI Technical Summary

Problems solved by technology

The growth and reproduction performance of this algae is very strong, and its growth requires a lot of space, so that other light-demanding algae organisms in the ocean cannot survive, resulting in the death of a large number of algae; the death of enteromorpha plants can cause eutrophication of the water body, and its degradation The process can consume a large amount of oxygen, resulting in a rapid decline in dissolved oxygen in the water, and fish suffocate and die due to lack of oxygen; eutrophication of the water body can also cause a large number of other algae to multiply, affecting the natural landscape of the coast

Therefore, the massive proliferation of Enteromorpha will cause serious damage to marine ecosystems

Images