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Preparation method of functional rare earth long-afterglow nanocomposite and latent fingerprint imaging application of functional rare earth long-afterglow nanocomposite

A technology of nanocomposite materials and nanomaterials, which is applied in the fields of biological analysis and fingerprint detection, can solve the problems of destructive samples, cumbersome and expensive processing, and achieve the effect of no background interference

Inactive Publication Date: 2016-02-03
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although fingerprint visualization technology plays an important role in criminal investigation and analytical science, there are still many difficulties in the field of fingerprint visualization
For example, in the traditional method, most of the latent fingerprints need to be referenced to an exogenous substance, and the processing process is cumbersome and destructive to the sample.
The small particle size powder used in the widely used smoke method and brushing method has certain physical damage to the technicians
However, emerging fingerprint technologies such as mass spectrometry and infrared Raman imaging require expensive large-scale instruments and professional operating knowledge, which is not conducive to popularization.

Method used

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  • Preparation method of functional rare earth long-afterglow nanocomposite and latent fingerprint imaging application of functional rare earth long-afterglow nanocomposite
  • Preparation method of functional rare earth long-afterglow nanocomposite and latent fingerprint imaging application of functional rare earth long-afterglow nanocomposite
  • Preparation method of functional rare earth long-afterglow nanocomposite and latent fingerprint imaging application of functional rare earth long-afterglow nanocomposite

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] according to figure 1 The schematic diagram in (a) shows the preparation of biotin-functionalized strontium aluminate rare earth doped long-lasting nanocomposites:

[0023] (1) Centrifuge the strontium aluminate rare earth-doped long-lasting nanomaterial at 3000rpm to take the supernatant, dry it in vacuum and grind it, take 1.6mg of strontium aluminate rare-earth doped long-lasting nanomaterial and disperse it in absolute ethanol and ultrasonically mix it. Add a mixed solution of 222 μL of ethanol, 247 μL of deionized water, and 6.7 μL of ethyl orthosilicate, sonicate for 5 minutes, add 25 μL of ammonia water (28%~30%), sonicate for 30 minutes, and stir magnetically at 1200 rpm for 7.5 hours. After centrifugation, they were washed with ethanol three times and dried under vacuum at 36°C to prepare silica-coated strontium aluminate rare earth-doped nanomaterials with long persistence.

[0024] (2) Disperse the material prepared in step (1) in 2mL N,N-dimethylformamide (...

Embodiment 2

[0030] Application of latent fingerprint imaging of biotin-functionalized strontium aluminate rare earth doped long-lasting nanocomposites: collect fingerprints on glass slides, drop 100 μL of a certain concentration of rabbit anti-lysozyme polyclonal antibody on latent fingerprints, and incubate at room temperature for 30 minutes. Through the specific recognition of the antigen / antibody, the rabbit anti-lysozyme polyclonal antibody binds to the lysozyme on the fingerprint ridge, and the incubated fingerprint sample is dipped in PBS buffer solution with pH 7.4 and gently shaken and washed twice. Remove non-specifically bound antibodies and blow dry with argon. Drop a certain concentration of biotin-labeled donkey anti-rabbit IgG 100 μL on the surface of the fingerprint, incubate for 30 minutes, and connect the biotin-labeled donkey anti-rabbit IgG to the fingerprint through the specific binding of the rabbit anti-lysozyme polyclonal antibody to the donkey anti-rabbit IgG The s...

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Abstract

The invention discloses a preparation method of functional rare earth long-afterglow nanocomposite and latent fingerprint imaging application of the functional rare earth long-afterglow nanocomposite and belongs to the technical field of biological analysis and fingerprint detection. Avidin is modified on the surface of an amino-functionalized strontium aluminate rare earth doped long-afterglow nano material, an avidin-functionalized rare earth long-afterglow nanocomposite is prepared, and therefore the avidin-functionalized rare earth long-afterglow nanocomposite is connected to a latent fingerprint marked by a biotinylated antibody through the recognition effect between biotin and avidin. The strontium aluminate rare earth doped long-afterglow nano material has the long-afterglow characteristic and still keeps emitting light after light source irradiation and light source removal, and latent fingerprint imaging can be achieved. The method is fast, simple and free of background interference.

Description

technical field [0001] The invention discloses a preparation method of a functionalized rare earth long-lasting nanocomposite material and its application in latent fingerprint imaging, belonging to the technical field of biological analysis and fingerprint detection. Background technique [0002] Latent fingerprints are fingerprint lines formed by the transfer of natural body secretions such as sweat, which are difficult to detect visually and are the most common fingerprints at crime scenes. According to incomplete statistics from various places in my country, among the criminal cases solved by the police, more than 70% are solved by using fingerprints, and the proportion is increasing year by year. Under normal circumstances, fingerprints are the most direct physical evidence left by criminals at the scene. Therefore, research on the display technology of latent fingerprints is an important link to improve the collection rate and utilization rate of fingerprint physical e...

Claims

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Application Information

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IPC IPC(8): C09K11/64C09K11/02A61B5/1172
Inventor 梁汝萍邱伟斌邱建丁
Owner NANCHANG UNIV
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