A kind of lipase 1-1 and its coding gene and application

A lipase gene and lipase technology, applied in the field of lipase L-1 and its coding gene and application, can solve the problems of expensive lipase, production technology constraints, etc.
CN105296446BInactive Publication Date: 2018-06-19SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Publication Date
2018-06-19
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses lipase L-1 and a coding gene and application thereof. The lipase gene l-1 is obtained from Streptomyces sp. SCSIO 13580 in a cloned mode, the nucleotide sequence of the lipase gene l-1 is shown as SEQ ID NO.1, the overall length of the lipase gene l-1 is 1005 bp, and the amino acid sequence of the coded lipase L-1 is shown as SEQ ID NO.2 and contains 334 amino acids in all. The lipase gene l-1 is cloned and connected with an expression vector pET-28a(+), then conversion is performed to obtain escherichia coli BL21 (DE3), and after culture and induced expression, the recombinant expression lipase L-1 is obtained. The lipase L-1 serving as a catalyst catalyzes a reaction between cinnamyl alcohol and an acyl donor, and cinnamyl acetate is prepared, wherein the yield of the obtained cinnamyl acetate can reach 48.3%. The lipase L-1 has the advantages of being high in stability and catalysis efficiency and can be applied to the fields of biological medicine, cosmetics, fine chemical engineering and the like.
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Description

technical field

[0001] The invention belongs to the fields of biochemical industry and biotechnology, and in particular relates to a lipase L-1 and its coding gene and application. Background technique

[0002] Cinnamyl acetate is one of the spice ingredients extracted from cinnamon, and it is a spice that can be used in my country. Most cinnamyl acetate is chemically synthesized, and only a small amount is extracted from natural plants. The chemical method has high requirements on conditions and needs to be completed under high temperature and high pressure. The consumption of equipment and energy consumption are high, and various by-products will be produced during the synthesis process. The subsequent extraction process is complicated and pollutes the environment. Enzyme as a biocatalyst has the advantages of mild reaction conditions, high specificity, less by-products, and less environmental pollution. It can overcome the above-mentioned shortcomings of chemical synthes...

Claims

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