Preparation method of silk fibroin two-layer periosteum-imitation material

A silk fibroin, membrane material technology, applied in the field of biomaterials, can solve the problem of not simulating the structure and function of the periosteum

Active Publication Date: 2016-02-17
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently reported periosteum-imitating materials are limited to the study of single-layer structural materials, and are rarely compounded with other active substances, and there is no good simulation of periosteum structure and function.

Method used

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  • Preparation method of silk fibroin two-layer periosteum-imitation material

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Effect test

Embodiment 1

[0023] (1) Preparation of outer layer spinning solution: after activation of VEGF (vascular endothelial growth factor), add VEGF / SF mass ratio of 2ng / mg to pure silk fibroin aqueous solution, stir and concentrate at 60°C to obtain SF with a concentration of 38% (Silk fibroin) / VEGF spinning solution;

[0024] (2) Preparation of the inner layer spinning solution: Add 0.1mol / L diammonium hydrogen phosphate aqueous solution to the silk fibroin aqueous solution with a concentration of 0.1%, then slowly add 0.1mol / L calcium nitrate solution, and adjust the pH= 10 or so, control the molar ratio of calcium to phosphorus to be 1.67, and control the mass ratio of silk fibroin to hydroxyapatite to be 0.2. After two hours of reaction, the solution is centrifuged to obtain a precipitate, which is then repeatedly washed with ethanol and freeze-dried to obtain SF / HA ( Hydroxyapatite) nanoparticles, and then add SF / HA nanoparticles to SF aqueous solution, stir and concentrate at 60°C to obtai...

Embodiment 2

[0028] (1) Preparation of outer layer spinning solution: after activation of VEGF (vascular endothelial growth factor), add VEGF / SF mass ratio of 10ng / mg to pure silk fibroin aqueous solution, stir and concentrate at 60°C to obtain SF with a concentration of 40% (Silk fibroin) / VEGF spinning solution;

[0029] (2) Preparation of the inner layer spinning solution: Add 0.1mol / L diammonium hydrogen phosphate aqueous solution to the silk fibroin aqueous solution with a concentration of 0.1%, then slowly add 0.1mol / L calcium nitrate solution, and adjust the pH= 10 or so, control the molar ratio of calcium to phosphorus to be 1.67, and control the mass ratio of silk fibroin to hydroxyapatite to be 0.2. After two hours of reaction, the solution is centrifuged to obtain a precipitate, which is then repeatedly washed with ethanol and freeze-dried to obtain SF / HA ( Hydroxyapatite) nanoparticles, and then add SF / HA nanoparticles to SF aqueous solution, stir and concentrate at 60°C to obta...

Embodiment 3

[0033](1) Preparation of outer layer spinning solution: after activation of VEGF (vascular endothelial growth factor), add VEGF / SF mass ratio of 20ng / mg to pure silk fibroin aqueous solution, stir and concentrate at 60°C to obtain SF with a concentration of 40% (Silk fibroin) / VEGF spinning solution;

[0034] (2) Preparation of the inner layer spinning solution: Add 0.1mol / L diammonium hydrogen phosphate aqueous solution to the silk fibroin aqueous solution with a concentration of 0.1%, then slowly add 0.1mol / L calcium nitrate solution, and adjust the pH= 10 or so, control the molar ratio of calcium to phosphorus to be 1.67, and control the mass ratio of silk fibroin to hydroxyapatite to be 0.2. After two hours of reaction, the solution is centrifuged to obtain a precipitate, which is then repeatedly washed with ethanol and freeze-dried to obtain SF / HA ( Hydroxyapatite) nanoparticles, and then add SF / HA nanoparticles to SF aqueous solution, stir and concentrate at 60°C to obtai...

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Abstract

The invention relates to a preparation method of a silk fibroin two-layer periosteum-imitation material. The preparation method comprises the steps of preparation of an outer-layer spinning solution, preparation of an inner-layer spinning solution and preparation of the two-layer periosteum-imitation material, wherein the obtained two-layer fibrous membrane is soaked in 70-100% methanol / water solution for aftertreatment to obtain the two-layer periosteum-imitation material which is insoluble in water. The prepared two-layer periosteum-imitation material can be used for repairing bone and wrapping a bone defect portion. The silk fibroin two-layer periosteum-imitation material has the advantages that the inner-layer base material and the outer-layer base material are the same, the layer compatibility is good, the inner layer and the outer layer are designed by stimulating the periosteum structure, the two-layer structure is provided, fibroin load growth factors are on the outer layer, fibroin composite hydroxyapatite is on the inner layer, and the fibroin load growth factors and the fibroin composite hydroxyapatite play the role of promoting angiogenesis and promoting bone growth ability, the two-layer periosteum is prepared through the electrospinning technology, and therefore the micro-nano fiber network structure of the periosteum can be better stimulated.

Description

technical field [0001] The invention relates to a preparation method of a periosteum-like material, in particular to a preparation method of a silk fibroin double-layered periosteum-like material. The method belongs to the field of biomaterials, and the prepared materials can be widely used in bone tissue repair. Background technique [0002] After the artificial bone repair material is implanted in the body, sufficient nutrient delivery is crucial for the degradation of the material, tissue regeneration, and vascularization. Studies have shown that the penetration thickness of tissue fluid in three-dimensional artificial bone scaffold materials is about 0.5 cm, but the thickness of scaffold materials is often greater than this value, so large scaffold materials are not conducive to the penetration of tissue fluid and the growth of cells into the deep layer of the scaffold. The main advantage of autologous bone is that there is a layer of periosteum on its surface, and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/44A61L27/22A61L27/12A61L27/54D04H1/4382D04H1/728
Inventor 何丹农周涓金林宇阳俊朱君金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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