Lactobacillus flexus for prevention and treatment of genitourinary tract infection in women

A technology for infection of Lactobacillus flexus and Staphylococcus, which is applied in the biological field and can solve problems such as toxic side effects and poor results

Active Publication Date: 2019-07-02
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor effect of antibiotics on genitourinary tract infections and the side effects of liver and kidney damage caused by antibiotics, efforts have been made to explore microecological therapies based on probiotic Lactobacillus preparations at home and abroad.

Method used

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  • Lactobacillus flexus for prevention and treatment of genitourinary tract infection in women
  • Lactobacillus flexus for prevention and treatment of genitourinary tract infection in women
  • Lactobacillus flexus for prevention and treatment of genitourinary tract infection in women

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1. Isolation and identification of Lactobacillus campylobacter strains

[0032] 1. Isolation of Lactobacillus campylobacter strains

[0033] MRS medium: peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; diammonium hydrogen citrate 2.0g; glucose 20.0g; Tween 80, 1.0mL; 3 COONa 3H 2 O) 5.0g; Dipotassium hydrogen phosphate (K 2 HPO4 3H 2 O) 2.0g; magnesium sulfate (MgSO 4 ·7H 2 O) 0.58g; manganese sulfate (MnSO 4 ·H 2 O) 0.25g; distilled water 1000mL; adjusted to pH 6.2~6.6.

[0034] More than 200 samples of vaginal secretions from healthy women with no symptoms and signs of vaginitis were collected, and the samples were numbered sequentially, inoculated in MRS liquid medium, and incubated anaerobic at 37°C for 24h. 5 single colonies were randomly selected from each sample and cultured in 2 mL MRS liquid medium for enrichment for 24 h, and the colonies were numbered sequentially. Amplified bacteria are used for bacterial seedling and DNA preparation. ...

Embodiment 2

[0045] Example 2. Functional analysis of Lactobacillus.crispatus IMC-25

[0046] 1. Detection of antibacterial ability of Lactobacillus.crispatus IMC-25

[0047] IMC-25 Lactobacillus activated overnight culture was inoculated into 10 mL of MRS liquid medium at a ratio of 10% and placed at 37 °C for 16 hours, and then incubated at 37 °C and 220r / min on a shaker for 3 hours, and the fermentation broth was filtered and sterilized Store at 4°C for later use.

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Abstract

The invention discloses Lactobacillus flexus for preventing and treating women's urogenital tract infection. The Lactobacillus campus provided by the present invention is specifically Lactobacillus campus (Lactobacillus. Growth of other conditional pathogenic bacteria; at the same time, it has strong adhesion to vaginal epithelial cells, ensuring the colonization of bacterial strains in vaginal epithelial cells. The Lactobacillus.crispatus IMC-25 provided by the present invention has a certain application prospect in the prevention and treatment of urogenital tract infection.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to Lactobacillus campylobacter and applications thereof, and particularly relates to Lactobacillus Campylobacter used for preventing and treating infection of the urogenital tract of women. Background technique [0002] Urogenital tract infection is a common disease that seriously affects the physical and mental health of women, mostly Escherichia coli or Staphylococcus aureus infection, with a prevalence rate as high as 40%; its treatment mainly relies on antibiotics, but the cure rate is low, and the recurrence rate can reach 40%. %above. [0003] In order to reduce the recurrence rate of urogenital tract infections, new treatment options are urgently needed. Due to the poor efficacy of antibiotics in the treatment of urogenital tract infections and the toxic and side effects of liver and kidney damage caused by antibiotics, efforts have been made at home and abroad to explore microecologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/74A61P31/04C12R1/225
CPCA61K35/747C12N1/205C12R2001/225
Inventor 朱宝利张瑞芬律娜王黎明李晶
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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