Sorghum variety breeding and cultivating method
A cultivation method and variety technology, applied in the field of sorghum cultivation, can solve problems such as lack of reasonable arrangements for planting timing and management, failure to increase sorghum yield, and impact on sorghum production, and achieve improved disease resistance, shortened growth cycle, and weak tillering Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0026] S1. Select high-quality, high-yielding sorghum varieties suitable for local planting, lodging resistance, weak tillers, large ears, and high seed setting rate;
[0027] S2. Adjust the concentrations of the expressed and purified Agrobacterium toxin proteins VirD1, VirD2, and VirE2 to 95 μg / μl respectively;
[0028] S3. After digesting the non-toxic broad-spectrum disease-resistant gene PYH157 and the insect-resistant gene CpT1 with restriction endonucleases, they were respectively connected to the pBin438 plasmid to construct two kinds of exogenous plasmid DNAs;
[0029] S4. Resuspending the obtained two kinds of exogenous plasmid DNAs in the plasmid DNA protecting agent respectively to obtain a mixed solution with a concentration of exogenous plasmid DNAs of 1-3 μg / μL;
[0030] S5. Take 2 μl of one or more of the solutions obtained in step S2, add the mixed solution obtained in step S4 containing 8 μl of exogenous plasmid DNA, and 6 μl of liposomes in sequence. After m...
Embodiment 2
[0037] S1. Select high-quality, high-yielding sorghum varieties suitable for local planting, lodging resistance, weak tillers, large ears, and high seed setting rate;
[0038] S2. Adjust the concentrations of the expressed and purified Agrobacterium toxin proteins VirD1, VirD2, and VirE2 to 90 μg / μl respectively;
[0039]S3. After digesting the non-toxic broad-spectrum disease-resistant gene PYH157 and the insect-resistant gene CpT1 with restriction endonucleases, they were respectively connected to the pBin438 plasmid to construct two kinds of exogenous plasmid DNAs;
[0040] S4. Resuspending the obtained two kinds of exogenous plasmid DNAs in the plasmid DNA protecting agent respectively to obtain a mixed solution with a concentration of exogenous plasmid DNAs of 1 μg / μL;
[0041] S5. Take 2 μl of one or more of the solutions obtained in step S2, add the mixed solution obtained in step S4 containing 8 μl of exogenous plasmid DNA, and 6 μl of liposomes in sequence. After mixi...
Embodiment 3
[0048] S1. Select high-quality, high-yielding sorghum varieties suitable for local planting, lodging resistance, weak tillers, large ears, and high seed setting rate;
[0049] S2. Adjust the concentrations of the expressed and purified Agrobacterium toxin proteins VirD1, VirD2, and VirE2 to 90-100 μg / μl, respectively;
[0050] S3. After digesting the non-toxic broad-spectrum disease-resistant gene PYH157 and the insect-resistant gene CpT1 with restriction endonucleases, they were respectively connected to the pBin438 plasmid to construct two kinds of exogenous plasmid DNAs;
[0051] S4. Resuspending the obtained two kinds of exogenous plasmid DNAs in the plasmid DNA protecting agent respectively to obtain a mixed solution with a concentration of exogenous plasmid DNAs of 3 μg / μL;
[0052] S5. Take 2 μl of one or more of the solutions obtained in step S2, add the mixed solution obtained in step S4 containing 8 μl of exogenous plasmid DNA, and 6 μl of liposomes in sequence. Afte...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com