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Sorghum variety breeding and cultivating method

A cultivation method and variety technology, applied in the field of sorghum cultivation, can solve problems such as lack of reasonable arrangements for planting timing and management, failure to increase sorghum yield, and impact on sorghum production, and achieve improved disease resistance, shortened growth cycle, and weak tillering Effect

Inactive Publication Date: 2016-02-24
山西双惠种业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Direct-seeding sorghum cultivation is one of the planting methods. This method is relatively common. At present, this method is also used in many places to plant sorghum. Most of them are conventional planting. There is no reasonable arrangement of planting timing and reasonable management, resulting in the production of sorghum planted. improve, the result is not ideal
[0003] Sorghum head smut is one of the most serious fungal diseases on sorghum. In severe cases, the incidence rate is as high as 90%, which greatly affects sorghum production.
Therefore, the use of many excellent sterile lines and restorer lines is limited or eliminated because they are not resistant to sorghum head smut
At present, no agent can effectively prevent and control sorghum head smut

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] S1. Select high-quality, high-yielding sorghum varieties suitable for local planting, lodging resistance, weak tillers, large ears, and high seed setting rate;

[0027] S2. Adjust the concentrations of the expressed and purified Agrobacterium toxin proteins VirD1, VirD2, and VirE2 to 95 μg / μl respectively;

[0028] S3. After digesting the non-toxic broad-spectrum disease-resistant gene PYH157 and the insect-resistant gene CpT1 with restriction endonucleases, they were respectively connected to the pBin438 plasmid to construct two kinds of exogenous plasmid DNAs;

[0029] S4. Resuspending the obtained two kinds of exogenous plasmid DNAs in the plasmid DNA protecting agent respectively to obtain a mixed solution with a concentration of exogenous plasmid DNAs of 1-3 μg / μL;

[0030] S5. Take 2 μl of one or more of the solutions obtained in step S2, add the mixed solution obtained in step S4 containing 8 μl of exogenous plasmid DNA, and 6 μl of liposomes in sequence. After m...

Embodiment 2

[0037] S1. Select high-quality, high-yielding sorghum varieties suitable for local planting, lodging resistance, weak tillers, large ears, and high seed setting rate;

[0038] S2. Adjust the concentrations of the expressed and purified Agrobacterium toxin proteins VirD1, VirD2, and VirE2 to 90 μg / μl respectively;

[0039]S3. After digesting the non-toxic broad-spectrum disease-resistant gene PYH157 and the insect-resistant gene CpT1 with restriction endonucleases, they were respectively connected to the pBin438 plasmid to construct two kinds of exogenous plasmid DNAs;

[0040] S4. Resuspending the obtained two kinds of exogenous plasmid DNAs in the plasmid DNA protecting agent respectively to obtain a mixed solution with a concentration of exogenous plasmid DNAs of 1 μg / μL;

[0041] S5. Take 2 μl of one or more of the solutions obtained in step S2, add the mixed solution obtained in step S4 containing 8 μl of exogenous plasmid DNA, and 6 μl of liposomes in sequence. After mixi...

Embodiment 3

[0048] S1. Select high-quality, high-yielding sorghum varieties suitable for local planting, lodging resistance, weak tillers, large ears, and high seed setting rate;

[0049] S2. Adjust the concentrations of the expressed and purified Agrobacterium toxin proteins VirD1, VirD2, and VirE2 to 90-100 μg / μl, respectively;

[0050] S3. After digesting the non-toxic broad-spectrum disease-resistant gene PYH157 and the insect-resistant gene CpT1 with restriction endonucleases, they were respectively connected to the pBin438 plasmid to construct two kinds of exogenous plasmid DNAs;

[0051] S4. Resuspending the obtained two kinds of exogenous plasmid DNAs in the plasmid DNA protecting agent respectively to obtain a mixed solution with a concentration of exogenous plasmid DNAs of 3 μg / μL;

[0052] S5. Take 2 μl of one or more of the solutions obtained in step S2, add the mixed solution obtained in step S4 containing 8 μl of exogenous plasmid DNA, and 6 μl of liposomes in sequence. Afte...

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PUM

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Abstract

The invention discloses a sorghum variety breeding and cultivating method. The method includes the following steps of introducing a non-toxic broad-spectrum anti-disease gene PYH157 and an insect-resistant gene CpTI into sorghum through a pollen tube channel method, selecting a positive plant, obtaining seeds of the plant, sun-drying, soaking and stirring the seeds, sowing the seeds, applying farmyard manure and base fertilizer, conducting early thinning and uniformizing to keep strong seedlings, conducting dispersing to keep the seedlings not dense but loose, pulling away sick, weak and hybrid seedlings, finally singling 2-3 seedlings in each pot according to the soil fertility, fertilizer applying level and sowing density when 5-6 leaves grow on each seedling, conducting hole-applied fertilizer dressing after the final singling, conducting the first time of intertillage soil loosening while weeding, conducting deep elongation and booting fertilizer dressing before elongation, and conducting the third time of intertillage ridging. The disease resistance of sorghum crops is improved, the growth cycle of sorghum is shortened, the maturity speed is higher, the disease resistance of the plant is high, yield is increased, quality is better, pods are plump, and plant diseases and insect pets are remarkably reduced.

Description

technical field [0001] The invention relates to the field of sorghum cultivation, in particular to a sorghum variety selection and cultivation method thereof. Background technique [0002] Sorghum is an annual herbaceous plant with thick, upright stalks and supporting roots on the base nodes; it likes warmth, is resistant to drought and waterlogging; it can be divided into edible sorghum, sugar sorghum, and broom sorghum according to its traits and uses. Edible sorghum grains are used for food and wine making; sorghum stalks can be used for sugar to make syrup or raw food; , can dry dampness and eliminate phlegm, calm the mind and calm the nerves. Belongs to economic crops. It is widely planted in the south of my country, especially in the mountains of the Yunnan-Guizhou Plateau. It is one of the main food products in this area, and it has largely solved the food security problem in this area. In order to increase the yield of sorghum, sorghum planting methods are also va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00A01G1/00
CPCA01G22/00C12N15/8279
Inventor 王淑芳冀月林刘建明杨存良武江鑫于伟邢玉伟高海生武江涛贾彦青王雄飞师丽晓聂建国张建萍柴小丽高美怀王宏伟贾变武龙王鑫郭俊峰丁一王云龙张鹏义段立功
Owner 山西双惠种业股份有限公司
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