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Method for preparing biochemical reagent peptone from pig blood cells

A technology of biochemical reagent and peptone, which is applied in the field of peptone preparation, can solve the problems of increased ash content and cumbersome steps, and achieve the effects of improving utilization rate, increasing yield and eliminating pollution

Inactive Publication Date: 2016-02-24
桐城市雨润生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the hydrolyzed hemoglobin products currently produced by pig blood cells need to adjust the pH value to below 5 after inactivating the enzyme in order to remove the hemoglobin to ensure the product color and quality. , so that the ash content increases and the steps are more cumbersome

Method used

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  • Method for preparing biochemical reagent peptone from pig blood cells

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Comparison scheme
Effect test

Embodiment 1

[0027] A method for preparing biochemical reagent peptone using pig blood cells as raw material, the specific steps are as follows:

[0028] 1) Blood cell pretreatment

[0029] Take 1020kg of blood cell solution obtained by centrifugation in a tube centrifuge, first add 1000kg of distilled water, stir for 5 hours for hemolysis; then add 1000kg of boiling water and stir for hemolysis for 60 minutes;

[0030] 2) Enzymolysis

[0031] Measure the pH of the pretreated blood cell dilution solution to 7.25, directly heat it to 55°C, add 2709 alkaline protease at a ratio of 2% of the original blood cell mass fraction, and enzymatically hydrolyze it for 6 hours, and then use it at a ratio of 1% of the original blood cell mass fraction. % ratio was added with 1398 neutral protease for 2 hours, followed by a compound protease reaction of 0.5% of the original blood cell fluid mass fraction for 2 hours;

[0032] 3) Inactivate enzyme

[0033] After the enzymatic hydrolysis is completed, ...

Embodiment 2

[0043] A method for preparing biochemical reagent peptone using pig blood cells as raw material, the specific steps are as follows:

[0044] 1) Blood cell pretreatment

[0045] Take 1000kg of blood cell solution obtained by centrifugation in a tube centrifuge, first add 1025kg of distilled water, stir for hemolysis for 10 hours; then add 1000kg of boiling water and stir for hemolysis for 30 minutes;

[0046] 2) Enzymolysis

[0047] The pH of the pretreated blood cell diluent was measured at 7.05, directly heated to 53°C, and 2709 alkaline protease was added at a ratio of 1.5% of the original blood cell mass fraction, and the enzymatic hydrolysis was carried out for 6 hours, and then the mass fraction of the original blood cell liquid was 1.5%. % ratio, add 1398 neutral protease to hydrolyze for 3 hours, and then add compound protease to react for 2 hours according to the ratio of 0.5% of the original blood cell mass fraction;

[0048] 3) Inactivate enzyme

[0049] After the...

Embodiment 3

[0059] A method for preparing biochemical reagent peptone using pig blood cells as raw material, the specific steps are as follows:

[0060] 1) Blood cell pretreatment

[0061] Take 1050kg of blood cell solution obtained by centrifugation in a tube centrifuge, first add 1000kg of distilled water, stir for 9 hours for hemolysis; then add 1020kg of boiling water and stir for 40 minutes for hemolysis;

[0062] 2) Enzymolysis

[0063] Measure the pH of the pretreated blood cell diluent at 7.45, heat to 54°C, add 2709 alkaline protease at a ratio of 2% of the original blood cell mass fraction, and enzymatically hydrolyze for 5 hours, and then account for 1.5% of the original blood cell mass fraction Add 1398 neutral protease to hydrolyze for 3 hours, and then add compound protease to react for 2 hours at a ratio of 0.8% of the original blood cell mass fraction;

[0064] 3) Inactivate enzyme

[0065] After the enzymatic hydrolysis is completed, heat the enzymatic hydrolysis solut...

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Abstract

The invention relates to a method for preparing a biochemical reagent peptone from pig blood cells. The method comprises the following steps: adding distilled water to a raw material which is a blood cell liquid obtained after centrifuging pig blood in order to dilute and hemolyze, carrying out boiling water denaturation, carrying out enzymatic hydrolysis by using protease, inactivating enzymes, carrying out plate-frame press filtration, taking the obtained filtrate, carrying out membrane concentration, carrying out vacuum concentration, and carrying out spray drying to obtain the pig blood peptone. The yield of the peptone product obtained in the invention is 80% or above, the peptone has the advantages of cheap and easily available raw materials needed in the production process, simple technology and high nutrition values, the total nitrogen content of the peptone is 14.5% or above, the amino nitrogen content of the peptone is 3% or above, and the peptone can be used as a raw material in the fermentation industry, the food industry and other light industries, improves the added values of the pig blood, and also can eliminate pollution brought by discharge of the pig blood to environment.

Description

technical field [0001] The invention relates to a method for preparing peptone, in particular to a method for preparing biochemical reagent peptone from pig blood cells. Background technique [0002] At present, domestic pig blood resources are very rich, but the effective utilization rate is not high. Most of them are used to make blood tofu, spray dried into blood plasma protein powder, and more are discharged into the surrounding environment, causing great pollution. Therefore, the effective utilization of pig blood still needs to be developed urgently. In the prior art, there is no disclosure of a method for preparing biochemical reagent peptone from pig blood cells. [0003] At present, the raw materials for preparing peptone are mainly animal and plant proteins and their leftovers such as meat, bones, fish skin, cheese, soybeans, etc., which are prepared by enzymatic hydrolysis technology. Compared with other animal and plant raw materials, pig blood cell raw material...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 杨风玲崔群维宁发子高仁清周全姜再胜
Owner 桐城市雨润生物科技有限公司
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