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Particle manipulation method and particle manipulation device

An operating method and technology for operating devices, applied in separation methods, peptide preparation methods, chemical instruments and methods, etc., can solve problems such as unusability and complex device structure, and achieve the effects of eliminating pollution, easy operation and efficient separation

Active Publication Date: 2016-02-24
SHIMADZU SEISAKUSHO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in this method, an electric field must always be applied to the device to immobilize the droplet, so there is a problem that the structure of the device is complicated and it cannot be used in an environment where energy such as electricity is insufficient.

Method used

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  • Particle manipulation method and particle manipulation device
  • Particle manipulation method and particle manipulation device
  • Particle manipulation method and particle manipulation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0136] In Example 1, genomic DNA was extracted from human whole blood using magnetic beads. As magnetic beads, a nucleic acid extraction kit manufactured by Toyobo (MagExtractor TM The magnetic beads attached to -Genome) were resuspended in distilled water to a concentration of 650 mg / Ml.

[0137]

[0138] First, one end of a polypropylene tube (inner diameter 2 mm, outer diameter 3.8 mm, manufactured by Renryi Co., Ltd.) was heated, melted and sealed to obtain a container. In this container, 150 μL of an eluent (distilled water: DNase, RNasefree; manufactured by Nacala Tissue Co., Ltd.), 20 μL of silicone gel (trade name “KSG-15”, manufactured by Shin-Etsu Chemical Co., Ltd.), and the second Cleaning solution (70% ethanol, 10mM Tris-HCl, 10mM EDTA, pH8.0) 150 μL, silicone gel (KSG-15) 20 μL, first cleaning solution (30% ethanol, 2M guanidine hydrochloride) 150 μL, and silicone gel Glue (KSG-15) 20μL. so, such as Figure 4 As shown in (a), the first gel-like medium 121, ...

Embodiment 2

[0159] In Example 2, preparation of a sample for enzyme immunochemical measurement was performed using magnetic beads. As the magnetic beads, protein G-coated magnetic particles (Dynabeads Protein G, manufactured by Life Sciences) were used.

[0160]

[0161] In order to coat the inner wall surface of borosilicate glass capillary tubes (manufactured by Hirschman Laboratories, ringcaps, 200 μl) with silicone, the tube is filled with a silicone coating agent (manufactured by Fuji Rika, siliconized L-25 solution ), the liquid was drawn off after 10 minutes. After washing with distilled water, the inside of the tube was dried with nitrogen gas. Then, the opening at one end of the capillary was sealed with a sealant (cha-seal). From the other opening of the capillary, 60 µL of a PBST solution (0.02% Tween20 containing phosphate-buffered saline) and 20 µL of silicone gel (KSG-15) were filled sequentially from the bottom. After this was repeated 3 times, 40 μL of PBST solution w...

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Abstract

This particle manipulation method has a step where particles present in an aqueous liquid are caused to move to the inside of a gelatinous medium that is insoluble or poorly soluble in the aqueous liquid, and a step where the particles present inside the gelatinous medium are caused to move to the outside of the gelatinous medium. Preferably, the gelatinous medium is a gel that contains a chemical crosslinking polymer. Also, preferably, the consistency of the gelatinous medium is 340 to 475. In one embodiment, the movement of the particles present in the aqueous liquid to the gelatinous medium and the movement of the particles inside the gelatinous medium to the aqueous liquid are carried out inside a device loaded with a plurality of aqueous liquids, the gelatinous medium being interposed between the aqueous liquids.

Description

technical field [0001] The present invention relates to a method for operating particles used for chemical operations such as separation, extraction, purification, and reaction of target substances, and an operating device used therefor. Background technique [0002] In medical inspection, food safety and sanitation management, monitoring for environmental protection, etc., it is required to extract target substances from samples containing various inclusions and use them for detection and reaction. For example, in medical examinations, it is necessary to detect nucleic acids, proteins, sugars, lipids, bacteria, viruses, radioactive substances, etc. contained in biological samples such as blood, serum, cells, urine, and feces separated from animals and plants , identification, quantification. When performing these inspections, it is sometimes necessary to separate and purify the target substance in order to eliminate negative effects such as background caused by inclusions....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09B01J19/08B03C1/12C07H21/04C07K1/14C12N1/00C12Q1/68G01N33/53
CPCB03C1/0332B03C1/288B03C2201/26C07K1/145C12N15/1013B03C1/01B01D15/1892C12Q1/6806B01D12/00
Inventor 大桥铁雄小原收野中谦
Owner SHIMADZU SEISAKUSHO LTD
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