Preparation method of microfluidic three-dimensional gel chip model

A microfluidic and gel technology, applied in chemical instruments and methods, laboratory containers, laboratory utensils, etc., can solve problems such as high cost, complicated preparation methods, and difficulty in realizing the construction of complex structural network shapes, and achieve Easy preparation and simple adjustment method

Inactive Publication Date: 2016-03-09
TAIYUAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The preparation methods of these technologies are complicated, the cost is high, a

Method used

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  • Preparation method of microfluidic three-dimensional gel chip model
  • Preparation method of microfluidic three-dimensional gel chip model
  • Preparation method of microfluidic three-dimensional gel chip model

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Agar-embedded calcium alginate columnar "mold" for perfusion of microfluidic 3D biogel single channel.

[0024] Ca used in the preparation of calcium alginate columnar "molds" 2+ The solution is provided by calcium chloride solution.

[0025] (1) Preparation of the microfiber columnar "mold": use a G31 syringe to inject a sodium alginate solution with a mass concentration of 1% into a calcium chloride solution with a concentration of 90 mmol / L at a rate of 100 μL / s at a uniform speed. The glue reacted for 15 minutes to form a columnar "mold" of calcium alginate.

[0026] (2) "Mold" embedded in three-dimensional agar gel: prepare 10ml of agar with a mass concentration of 10mg / mL. Melt and sterilize at 121°C in an autoclave. Immediately after sterilization, take out 5ml of agar and spread it evenly on the petri dish. After cooling, a single calcium alginate "mold" is placed on the gel with injection ports at the beginning and end of the gel. Use 5ml of agar solution ...

Embodiment 2

[0030] Agar-embedded calcium alginate columnar "mold" to prepare perfusion microfluidic three-dimensional biogel dual channels.

[0031] Ca used in the preparation of calcium alginate columnar "molds" 2+ The solution is provided by calcium nitrate solution.

[0032] (1) Preparation of microfiber columnar "mold": Using a G22 syringe, inject a sodium alginate solution with a mass concentration of 1.5% into a calcium nitrate solution with a concentration of 60 mmol / L at a constant speed of 140 μL / s, and the gel After 40 minutes of reaction, a calcium alginate columnar "mold" was formed.

[0033] (2) "Mold" embedded in three-dimensional agar gel: Prepare 10ml of agar with a mass concentration of 15mg / mL. Melt and sterilize at 121°C in an autoclave. Immediately after sterilization, take out 5ml of agar and spread it evenly on the petri dish. After cooling, place two calcium alginate "molds" crosswise on the gel, and reserve injection ports at the beginning and end of the gel. ...

Embodiment 3

[0037] Gelatin-embedded calcium alginate columnar "mold" for perfusion of microfluidic 3D biogel single channel.

[0038] Ca used in the preparation of calcium alginate columnar "molds" 2+ The solution is provided by calcium chloride solution.

[0039] (1) Preparation of microfiber columnar "mold": use a G33 syringe to inject a sodium alginate solution with a mass concentration of 1.2% into a calcium chloride solution with a concentration of 120 mmol / L at a rate of 80 μL / s at a uniform speed, The gel was reacted for 35 minutes to form a columnar “mold” of calcium alginate.

[0040] (2) The "mold" is embedded in a three-dimensional gelatin gel: prepare 10ml of gelatin with a mass concentration of 12mg / mL. Melt and sterilize at 121°C in an autoclave. Immediately after sterilization, take out 5ml of gelatin and spread it evenly on the petri dish. After cooling, a single calcium alginate "mold" is placed on the gel with injection ports at the beginning and end of the gel. Slo...

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Abstract

The invention discloses a preparation method of a microfluidic three-dimensional gel chip model and belongs to the field of biological micro-processing. A preparation process comprises the following steps: (1) preparing a micro-fiber columnar mold; (2) embedding the prepared mold into a three-dimensional biological gel; (3) dissolving the mold and constructing a micro-channel in the three-dimensional biological gel so as to form the microfluidic three-dimensional gel chip model. A method for constructing a microfluidic chip in the three-dimensional biological gel is realized and the method has a simple preparation process; the size and space structure of the formed micro-channel can be adjusted according to actual requirements. The microfluidic three-dimensional gel chip model can be used for research in the fields of tissue microenvironments, vascular tissue engineering, 3D drug delivery and screening and the like.

Description

technical field [0001] The invention relates to a method for preparing a microfluidic three-dimensional gel chip model, which belongs to the technical field of biological microprocessing. Background technique [0002] In scientific experiments such as biology, chemistry, and materials, it is often necessary to manipulate fluids. Hydrogels with microchannel structures can play a very important role in the preparation of functional materials, as well as in the simulation and reconstruction of vascularized tissues in vitro. The vasculature in living organisms can be simulated by fabricating suitable microchannel structures inside the hydrogel. In addition, by changing the components of the hydrogel, applications such as microchannel screening and biochip detection can also be realized. [0003] At present, the construction methods of three-dimensional channels mainly use photolithography technology, 3D printing technology and so on. The preparation methods of these technolog...

Claims

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Application Information

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IPC IPC(8): B01L3/00
Inventor 黄晓波杨晓宁杭瑞强秦林唐宾
Owner TAIYUAN UNIV OF TECH
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