A kind of method that uses lignin sulfonate as inducer to improve the enzyme production of Trametes
The technology of a lignosulfonate and an inducer is applied in the field of microbial fermentation, can solve the problems of not involving a multi-enzyme system, the enzyme activity of the fermentation crude enzyme liquid needs to be improved, etc., and achieves the effect of a wide range of sources
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Embodiment 1
[0039] Embodiment 1: Trametes fermentation produces enzyme
[0040] Weigh 200g of peeled potatoes, cut into small pieces, add 1.0L of water and boil for 30min, filter the potato pieces through 4 layers of gauze, make up the filtrate to 1.0L, add 20.0g of glucose, add 0.8g of lignosulfonate, fully dissolve . After subpackaging, sterilize under high pressure at 121°C for 20min, cool down, perform aseptic operation, insert the bacterial suspension of Trametessp. Under the shaking culture for 6 days, the rotation speed is 150rpm. After the fermentation was over, the fermentation broth was centrifuged at 4000rpm for 30min, and the supernatant was taken to measure the enzyme activity. Live 16800U / L.
Embodiment 2
[0043] Embodiment 2: Trametes fermentation produces enzyme
[0044] Weigh 200g of peeled potatoes, cut into small pieces, add 1.0L of water and boil for 30min, filter through 4 layers of gauze to remove the potato pieces, make up the filtrate to 1.0L, add 20.0g of glucose and 1g of lignosulfonate to fully dissolve. After subpackaging, sterilize under high pressure at 121°C for 20 minutes, cool down, perform aseptic operation, insert the bacterial suspension of the Trametessp.LEF01 strain that has been cultured on the PDA slant medium for 5 to 6 days, and place it in a constant temperature shaking incubator at 28°C. Under the shaking culture for 6 days, the rotation speed is 150rpm. After the fermentation was over, the fermentation broth was centrifuged at 4000rpm for 30min, and the supernatant was taken to measure the enzyme activity. Live 19800U / L.
[0045] Wherein, the control is as follows: no lignosulfonate is added to the medium, and other steps are consistent with Exam...
Embodiment 3
[0047] Embodiment 3: the influence of inducer type on fermented enzyme production of Trametes
[0048] 2mmol guaiacol was used to replace sodium lignosulfonate, other steps were consistent with Example 3, the results showed that in the supernatant of the fermentation broth, the activity of laccase was 730U / L, the activity of hemicellulase was 3076U / L, and pectin Enzyme activity 1090U / L, no cellulase was detected. Compared with no inducer, the laccase activity increased to 1.16 times, the hemicellulase activity did not increase, and the pectinase activity increased to 2.09 times.
[0049] 2mmol alkali lignin was used to replace lignosulfonate, other steps were consistent with Example 3, the results showed that in the supernatant of fermentation broth, laccase activity was 873U / L, hemicellulase activity was 3200U / L, and pectinase activity was 3200U / L. The enzyme activity was 490U / L, and no cellulase was detected. Compared with no inducer, the enzyme activity of laccase increas...
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