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Lactobacillus. crispatus strain, and microecological preparation thereof

A technology of Lactobacillus flexus and bacterial suspension, applied in the direction of microorganisms, microorganisms, methods based on microorganisms, etc., can solve problems such as poor effect and toxic side effects

Inactive Publication Date: 2016-03-16
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the poor effect of antibiotics on genitourinary tract infections and the side effects of liver and kidney damage caused by antibiotics, efforts have been made to explore microecological therapies based on probiotic Lactobacillus preparations at home and abroad.

Method used

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  • Lactobacillus. crispatus strain, and microecological preparation thereof
  • Lactobacillus. crispatus strain, and microecological preparation thereof
  • Lactobacillus. crispatus strain, and microecological preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, isolation and identification of Lactobacillus flexus strain

[0032] 1. Isolation of Lactobacillus flexus strains

[0033] MRS medium: peptone 10.0g; beef extract 10.0g; yeast extract 5.0g; diammonium hydrogen citrate 2.0g; glucose 20.0g; Tween 80, 1.0mL; sodium acetate (CH 3 COONa·3H 2 O) 5.0g; Dipotassium hydrogen phosphate (K 2 HPO4·3H 2 O) 2.0g; Magnesium sulfate (MgSO 4 ·7H 2 O) 0.58g; manganese sulfate (MnSO 4 ·H 2 O) 0.25g; distilled water 1000mL; adjust to pH6.2~6.6.

[0034] More than 200 samples of vaginal secretions from healthy women without symptoms and signs of vaginitis were collected. The samples were numbered sequentially, inoculated in MRS liquid medium, and incubated anaerobically at 37°C for 24 hours. For each sample, 5 single colonies were randomly selected and cultured in 2 mL MRS liquid medium for 24 hours, and the colonies were numbered sequentially. Bacteria are amplified for bacterial conservation and DNA preparation. Hu...

Embodiment 2

[0045] Example 2, Functional analysis of Lactobacillus crispatus (Lactobacillus.crispatus) IMC-159

[0046] 1. Production of H by Lactobacillus.crispatus IMC-159 2 o 2 detection

[0047] h 2 o 2 Standard curve making: first use piperazine-N, N'-di-ethanesulfonic acid (Piperazine-N, N_-Bis2-ethanesulfonic acid, PIPES) with a concentration of 100mmol / L to dissolve 30% (v / v) H 2 o 2 (equivalent to 9.128mol / L) stock solution diluted to 1mol / L, and then 1mol / LH with 100mmol / LPIPES 2 o 2 Dilute to 0μmol / L, 20μmol / L, 40μmol / L, 60μmol / L, 80μmol / L, 100μmol / L working solution; then take 100μL of the above H 2 o 2 Mix the working solution with 100 μL of TMB (tetramethylbenzidine) at a concentration of 20 mmol / L and 2 μL of horseradish peroxidase (1 mg / mL), incubate at 16°C for 10 min, and measure the OD 630 value. end up with H 2 o 2 Concentration standard curve: y=0.0033x-0.0496, R 2 = 0.992.

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Abstract

The invention discloses a Lactobacillus. crispatus strain, and a microecological preparation thereof. The Lactobacillus. crispatus strain is Lactobacillus. crispatus IMC-159, possesses excellent H2O2 producing capacity, and excellent adhesive attraction on vaginal epithelial cells, and is capable of ensuring colonization of bacterial strains on vaginal epithelium. The Lactobacillus. crispatus IMC-159 possesses a certain application prospect in preventing and treating urogenital tract infection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a strain of Lactobacillus flexus and a microecological preparation thereof, in particular to a strain of Lactobacillus flexus isolated from a human body and used for preventing and treating urogenital tract infection in women. Background technique [0002] Urogenital tract infection is a common disease that seriously affects the physical and mental health of women. Most of them are Escherichia coli or Staphylococcus aureus infection, and the prevalence rate is as high as 40%. Its treatment mainly relies on antibiotics, but the cure rate is low, and the recurrence rate can reach 40%. %above. [0003] In order to reduce the recurrence rate of genitourinary tract infection, new treatment options are urgently needed clinically. Due to the ineffectiveness of antibiotics in the treatment of urogenital tract infections and the side effects of liver and kidney damage caused by antibiotics, ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P13/02A61P15/02A61P31/04C12R1/225
CPCA61K35/747C12N1/205C12R2001/225
Inventor 朱宝利张瑞芬律娜胡永飞王黎明李晶
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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