A ps@sio for the detection of paraquat molecules 2 Preparation method of artificial antibody

Abstract

The invention provides a preparation method of a PS@SiO2 artificial antibody for detecting paraquat molecules. The preparation method is characterized in that mark molecules in a SiO2 shell layer are eluted from the artificial antibody; a hole structure which can be complementary with structures, sizes and functions of the mark molecules is formed in the SiO2 shell layer; the artificial antibody has specific identification sites of target analyte molecules and selective identification and detection can be realized. The preparation method comprises the following two steps: firstly, synthesizing a mono-dispersed PS micro-sphere carrying a carboxyl functional monomer on the surface; then covering one SiO2 shell layer with a controllable shell thickness on the surface of the mono-dispersed PS micro-sphere through the static mutual effect of the carboxyl on the surface and the paraquat molecules; and eluting paraquat to form the artificial antibody for identifying and detecting the paraquat molecules. The nano shell layer has controllable thickness, strong rigidity and large specific surface area; the space for forming the identification sites has a stable structure, a plurality of effective sites and good selectivity, and has high identification efficiency on the target molecules.

Description

technical field [0001] The invention relates to the field of material science, in particular to PS@SiO for detecting paraquat molecules 2 Preparation method of artificial antibody. Background technique [0002] Pesticides play a huge role in the prevention and control of agricultural diseases, insects, and weeds, and in ensuring high yields and incomes of crops. At the same time, they also pose a serious threat to the ecological environment and human health, which has attracted great attention from governments and the international community. According to statistics, there are about 1.5 billion cases of food quality-induced cases in the world every year, and about 3 million deaths, especially the residues of pesticides in agricultural and animal husbandry products and food, which have chronic toxicity to the human body and "three causes" ( carcinogenic, teratogenic, mutagenic) effects and environmental hormone effects. Incidents of poisoning directly caused by residues of ...

AI Technical Summary

Problems solved by technology

[0007] However, currently imprinted polymers prepared by traditional methods face many difficulties that need to be overcome in the practical application of molecular recognition and signal output, which can be summarized into the following aspects: (1) due to the crosslinking density of molecularly imprinted polymers High, template molecules inside the cross-linked network cannot be completely removed (Markowitz, M. A.; Kust, P. R.; Deng, G.; Schoen, P. E.; Gaber, B. P. Langmuir 2000, 16,.1759.; Rao, M. S.; Dave, B. C. J. Am. Chem. Soc. 1998, 120, 13270.); (2) due to the small number of effective sites, the affinity for target molecules is small; (3) it is difficult for target molecules to diffuse into the imprinted sites inside the network, so the binding kinetics for target molecules is slow ; (4) Molecularly imprinted polymers are usually irregularly shaped materials, which have poor compatibility with sensing devices

(5) The target analyte enters the recognition site itself and cannot output a sensitive signal. Therefore, molecularly imprinted polymers synthesized by traditional methods often exhibit high selectivity, low binding capacity, poor site accessibility, and slow binding. Kinetics and the target analyte entering the recognition site itself cannot output a sensitive signal. In view of this, the synthesis of high selectivity, high binding capacity, easy access to the site, fast binding kinetics and highly sensitive signal output has a regular morphology. Molecularly imprinted polymer materials have been the goal of people's pursuit (Hayden, O.; Mann, K. J.; Krassnig, S.; Dickert, F. L. Angew. Chem. Int. Ed. 2006, 45 , 2626.; Schmidt, R. H.; Mosbach, K.; Haupt, K. Adv. Mater. 2004, 16 , 719.)

Although the Elham Effati research group mentioned in the article that the repeated polymerization steps increase the thickness of the shell, the operation process is cumbersome

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