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Theasaponin extract and preparation method thereof

A technology of tea saponins and extracts, applied in the preparation of anti-inflammatory and hypolipidemic health food or medicines, in the field of triterpenoid saponins, can solve problems such as hindering the popularization and application of extracts, difficult quality control, weak water solubility and the like , to achieve the effect of small dosage, improved physical and chemical properties, and clear composition

Active Publication Date: 2016-03-23
SUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, since the natural tea saponin contains a variety of substituted ester groups, its water solubility is weak, which hinders the further application of the extract; the method of physically and chemically modifying tea saponin is the only way to expand its application range. One of the research hotspots [Song Binglei, Preparation and properties of deesterified tea saponin, Biomass Chemical Engineering, 2011, 45(5), 8-10; Qian Wei, Research progress in the preparation and application of modified tea saponin derivatives, Economic Forest Research, 2012, 30(4), 168-173]
[0004] Aiming at the defects that the existing tea saponin products have complex composition and difficult quality control, the inventor designed and adopted alkaline hydrolysis method to selectively remove the ester group in tea saponin according to its structural characteristics, and combined with macroporous resin, reversed-phase silica gel and other column layers Analysis and preparation technology, in order to obtain a new product of tea saponin with stable chemical composition and controllable quality

Method used

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  • Theasaponin extract and preparation method thereof
  • Theasaponin extract and preparation method thereof
  • Theasaponin extract and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Preparation of Camellia Seed Saponins

[0025] The cake obtained from oil tea seed (Camelliaoleifera, batch 20120901, collected from Guangnan, Yunnan) is selected.

[0026] (1) Weigh 800 g of camellia seed cake, pulverize and add 10 times the amount of 80% ethanol to soak overnight, reflux and extract twice for 2 hours each time, and concentrate to about 3L under reduced pressure. Take 2L of the concentrated solution and absorb it on AB-8 macroporous adsorption resin column (15×60cm, column volume 2L), followed by water (8L), 10% ethanol (8L), 30% ethanol (8L), 75% ethanol ( 8L) eluted, and the 75% ethanol eluted part was concentrated under reduced pressure and dried to obtain 40.9 g of total saponin CSA.

[0027] (2) Take 10g of CS-A, add 100ml of 2mol / L NaOH solution, hydrolyze at 95℃ for 5h, neutralize with 5mol / L HCl to pH 4-6, load AB-8 macroporous adsorption resin (column volume 400ml), and then use water (1.6L) ), 75% ethanol (1.6L), the 75% ethanol eluate ...

Embodiment 2

[0038] Example 2: Preparation of Camellia oleifera Root Saponins

[0039] The root of Camellia oleifera (batch 20110901, collected from Qichun County, Hubei Province) was selected and prepared with reference to Example 1.

[0040] (1) Weigh 100g of Camellia oleifera root, pulverize and add 10 times the amount of 60% ethanol to soak overnight, reflux and extract twice, 1.5h each time, concentrate to 500mL under reduced pressure, and put on AB-8 macroporous adsorption resin column (column volume 300mL ) After adsorption, eluted with water (5L), 30% ethanol (1L), 60% ethanol (1L), and the 60% ethanol eluted part was concentrated under reduced pressure and dried to obtain 21.4g of total saponin CSA.

[0041] (2) Take 10g of CSA, add 100ml of 2mol / L NaOH solution, hydrolyze at 95°C for 5 hours, neutralize with 5mol / L HCl to pH 4-6, load AB-8 macroporous adsorption resin (column volume 400ml), and then water (1.6L), 75% ethanol (1.6L), the 75% ethanol eluate was collected, concentrated un...

Embodiment 3

[0044] Example 3: Preparation of tea seed saponin

[0045] The seeds of tea (Camelliasinensis, batch 20131001, collected from Guangnan, Yunnan) were selected and prepared with reference to Example 1.

[0046] (1) Weigh 100g of tea seeds, pulverize and add 10 times the amount of 60% ethanol to soak overnight, reflux and extract twice, 1.5h each time, concentrate to 500mL under reduced pressure, and put on AB-8 macroporous adsorption resin column (column volume 300mL ) After adsorption, eluted with water (5L), 30% ethanol (1L) and 60% ethanol (1L) successively, and the 60% ethanol eluted part was concentrated under reduced pressure and dried to obtain 27g of total tea saponin CSA.

[0047] (2) Take 10g of CSA, add 100ml of 2mol / L NaOH solution, hydrolyze at 95°C for 5 hours, neutralize with 5mol / L HCl to pH 4-6, load AB-8 macroporous adsorption resin (column volume 400ml), and then water (1.6L), 75% ethanol (1.6L), the 75% ethanol eluate was collected, concentrated under reduced press...

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Abstract

The invention discloses a triterpenoid saponin compound shown by a formula (I) and a preparation method thereof. The triterpenoid saponin compound is prepared by the following main steps: selecting a camellia plant raw material, performing extraction by adopting water or an alcohol-water mixed solvent, and performing macroporous resin column enrichment to obtain a theasaponin extract CSA; performing hydrolytic reaction on CSA in an alkali solution of which the pH value is 10-13, performing acid neutralization, and then performing macroporous resin column refining to obtain a theasaponin extract CSB; and separating CSB by adopting a reverse phase silica gel column chromatography technology to obtain the triterpenoid saponin compound and a composition thereof. The invention simultaneously discloses an application of the theasaponin extract in preparation of medicines and health foods, and is characterized in that the theasaponin extract has pharmacological actions of anti-inflammation, anti-hyperlipidemia and the like. The theasaponin provided by the invention is clear and definite in active ingredient, stable and controllable in quality and concise in preparation process, is easy for industrial amplification, and has a good application value.

Description

Technical field [0001] The present invention relates to a triterpene saponin compound extracted from a Camellia plant. The present invention also relates to the application of the triterpene saponin compound in the preparation of anti-inflammatory and hypolipidemic health food or medicine, belonging to the field of biomedicine. technical background [0002] Camellia (Camellia) plants are widely distributed in the hilly regions of southern China and other Southeast Asian countries, and have been cultivated in China for more than two thousand years. Tea is a famous specialty in China. In addition, the oil can be extracted from the seeds of the Camellia plant, and the obtained camellia oil has a content of unsaturated fatty acids as high as 90%, which has high nutritional value; the tea saponins content in the tea seed cake from which the tea oil is extracted is about 10%. A rich variety of tea saponins are also found in the seeds, roots, flower buds and leaves of Camellia plants. ...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P29/00A61P3/06A23L33/11
Inventor 刘江云王增尚陆昊许琼明郝丽莉
Owner SUZHOU UNIV
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