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Method for biosynthesizing nano-selenium through bacillus licheniformis

A Bacillus licheniformis and biosynthetic technology, applied in the biological field, can solve the problems of unfavorable industrial production and low tolerance to selenite, and achieve the effects of simple medium and culture conditions, convenient preparation, and simple operation

Active Publication Date: 2016-03-23
SICHUAN ZHONGNONG HERUN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the bacillus mentioned in the existing reports is not high in tolerance to selenite, and is also unfavorable for industrialized production.

Method used

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  • Method for biosynthesizing nano-selenium through bacillus licheniformis
  • Method for biosynthesizing nano-selenium through bacillus licheniformis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: A method utilizing bacillus licheniformis to biosynthesize nano selenium, it comprises the following steps:

[0024] S1. Seed culture on slant: inoculate Bacillus licheniformis on the slant of nutrient agar medium, and cultivate it at 28°C for 36 hours to obtain slant seeds; nutrient agar medium is prepared from raw materials with the following concentrations: beef extract: 2g / L, Peptone: 8g / L, sodium chloride: 3g / L, agar: 12g / L, pH value is 7.0;

[0025] S2. Primary seed culture: inoculate the slanted seeds in the liquid fermentation medium, 100 ml of the liquid fermentation medium is housed in a 1L Erlenmeyer flask, vibrate at 28° C. for 12 hours, and the speed of the vibration culture is 180 rpm to obtain the first-grade liquid seeds;

[0026] S3. Secondary seed cultivation: Inoculate the primary liquid seed into a 300L liquid fermentation tank filled with 210L liquid fermentation medium, vibrate and cultivate at 28°C for 12 hours, and the speed of the v...

Embodiment 2

[0029] Example 2: A method utilizing bacillus licheniformis to biosynthesize nano selenium, it comprises the following steps:

[0030] S1. Seed culture on slant: inoculate Bacillus licheniformis on the slant of nutrient agar medium, and cultivate it at 45°C for 48 hours to obtain slant seeds; nutrient agar medium is prepared from raw materials with the following concentrations: beef extract: 5g / L, Peptone: 15g / L, sodium chloride: 7g / L, agar: 18g / L, pH value is 7.2;

[0031] S2. Primary seed cultivation: inoculate the slant seeds in the liquid fermentation medium, 100 ml of the liquid fermentation medium is housed in a 1L Erlenmeyer flask, vibrate at 45° C. for 18 hours, and the speed of the vibration cultivation is 220 rpm to obtain the first-grade liquid seeds;

[0032] S3. Secondary seed cultivation: inoculate the primary liquid seed into a 300L liquid fermentation tank filled with 210L liquid fermentation medium, vibrate and cultivate at 45°C for 18 hours, and the speed o...

Embodiment 3

[0035] Example 3: A method utilizing bacillus licheniformis to biosynthesize nano selenium, it comprises the following steps:

[0036] S1. Seed culture on slant: inoculate Bacillus licheniformis on the slant of nutrient agar medium, and cultivate it at 34°C for 40 hours to obtain slant seeds; nutrient agar medium is prepared from raw materials with the following concentrations: beef extract: 3g / L, Peptone: 10g / L, sodium chloride: 5g / L, agar: 14g / L, pH value is 7.1;

[0037]S2. Primary seed culture: inoculate the slant seeds in the liquid fermentation medium, 100 ml of the liquid fermentation medium is housed in a 1L Erlenmeyer flask, vibrate at 34° C. for 14 hours, and the speed of the vibration culture is 200 rpm to obtain the first-grade liquid seeds;

[0038] S3. Secondary seed cultivation: Inoculate the first-stage liquid seed into a 300L liquid fermentation tank filled with 210L liquid fermentation medium, and vibrate at 34°C for 14 hours at a speed of 200rpm to obtain ...

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Abstract

The invention discloses a method for biosynthesizing nano-selenium through bacillus licheniformis. The method comprises the steps of slope seed culture, first-level seed culture, second-level seed culture and fermentation production. The selected bacillus licheniformis can resist high-concentration selenite and can resist sodium selenite with the highest concentration of 2000 mg / L. The efficiency of converting the sodium selenite into red active nano-selenium reaches up to 80% or above. The bacillus licheniformis belongs to probiotics, is safe to use and can be directly used as a feed additive to be added into livestock and poultry feed, and selenium-enriched meat food can be produced for people by feeding livestock and poultry with the nano-selenium produced through the bacillus licheniform. According to the selected bacillus licheniformis, the requirements for culture media and culture conditions needed during culture are low, the culture cost is low, and the method for biosynthesizing the nano-selenium through the bacillus licheniformis has the advantages that operation is easy, preparation is convenient and the cost is low, and is applicable to industrial large-scale production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for biosynthesizing nanometer selenium by using Bacillus licheniformis. Background technique [0002] Selenium is an essential trace element of great importance to the health of living organisms. Selenium is an important building block of selenoproteins and plays an important role in its structure and enzymes, as an antioxidant and catalyst for the production of active thyroid hormones is the best example. In the human body, selenium can promote the normal operation of the immune system, effectively inhibit the amplification of HIV, and at the same time, it can also enhance sperm motility, improve reproductive performance, reduce the probability of miscarriage, and have anti-cancer effects. Selenium deficiency often puts the body in a negative emotional state, making it susceptible to disease and viral infection. [0003] The selenium of the human body mainly c...

Claims

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Application Information

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IPC IPC(8): C12P3/00C12N1/20C12R1/10
CPCC12N1/20C12P3/00
Inventor 彭锋韩江学
Owner SICHUAN ZHONGNONG HERUN TECH CO LTD
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