Method for extracting sardine polypeptide

A technology of sardines and sardine meat, which is applied in fermentation and other directions, can solve safety concerns, kidney toxicity and side effects, and achieve the effects of low cost, wide source of raw materials, and high medicinal economic value

Inactive Publication Date: 2016-03-23
广西钦州市绿源天然食品加工有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Drugs currently used for the treatment of hypertension mainly include angiotensin converting enzyme (Angiotensin converting enzyme, ACE) inhibitors such as

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  • Method for extracting sardine polypeptide
  • Method for extracting sardine polypeptide

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Effect test

Embodiment 1

[0024] (1) Clean the sardines, remove their heads and scales, put them into a high-speed tissue masher and stir them into a minced meat shape, and dissolve them in a constant temperature water bath at 37°C for 3 hours;

[0025] (2) Add the minced sardine meat to distilled water at a concentration of 20% (W / V) and stir evenly, add it to the enzymolysis tank, raise the temperature to 45°C, and add the minced sardine meat to the pancreas according to the weight of 2000U / g. Protease, adjust the pH with acid and alkali to keep the pH at 7.5, stir at 1000r / min at a constant speed, heat for 1.5h after enzymatic hydrolysis, then add 3000U / g papain, heat up to 55℃, adjust with acid and alkali to keep the pH at 6.5, 1000r Stir at constant speed / min, keep warm for 2h;

[0026] (3) After the enzymolysis is completed, place the enzymatic hydrolysate in a constant temperature water bath at 95°C for 15 min. After cooling, centrifuge at 5000r / min for 15 min. Take the supernatant and place it in a...

Embodiment 2

[0028] (1) Clean the sardines, remove their heads and scales, put them in a high-speed tissue masher and stir them into a minced meat shape, and dissolve them in a constant temperature water bath at 37°C for 2 hours;

[0029] (2) Add the minced sardine meat to distilled water at a concentration of 25% (W / V) and stir evenly, add it to the enzymolysis tank, raise the temperature to 50℃, and add the minced sardine meat to the pancreas at 2500U / g based on the weight of the minced sardine. Protease, adjust with acid and alkali to keep the pH at 8.0, stir at 2000r / min at a constant speed, after incubating for 2h, add 4000U / g papain, heat to 60℃, adjust with acid and alkali to keep the pH at 7.0, 2000r / Stir at a constant speed for min, keep warm for 2.5h;

[0030] (3) After the enzymolysis is completed, put the enzymatic hydrolysate in a constant temperature water bath at 98°C for 18 min. After cooling, centrifuge at 6000r / min for 18 min. Take the supernatant and place it in a rotary eva...

Embodiment 3

[0032] (1) Clean the sardines, remove their heads and scales, put them in a high-speed tissue mixer and stir them into a minced meat shape, and dissolve them in a constant temperature water bath at 37°C for 2.5 hours;

[0033] (2) Add the minced sardine meat to distilled water at a concentration of 30% (W / V) and stir it evenly, add it to the enzymolysis tank, raise the temperature to 55℃, and add 3000U / g to the pancreas based on the weight of the minced sardine meat. Protease, adjust with acid and alkali to keep the pH at 8.5, stir at 3000r / min at a constant speed, incubate for 2.5h after enzymatic hydrolysis, then add 2500U / g of papain, heat up to 65℃, adjust with acid and alkali to keep the pH at 7.3, 3000r Stir at a constant speed / min, heat preservation and enzymatic hydrolysis for 2h;

[0034] (3) After the enzymolysis is completed, place the enzymatic hydrolysis solution in a constant temperature water bath at 98°C for 20 min. After cooling, centrifuge at 7000r / min for 20 min...

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Abstract

The invention provides a method for extracting sardine polypeptide. The method comprises the following steps: carrying out enzymatic hydrolysis step by step through trypsin and papain, then heating to kill enzymes, performing centrifugation, subjecting the supernate to rotary evaporation, and carrying out freeze-drying to obtain the sardine enzyme. The method is not limited by the enzyme specificity of enzymatic method, has the advantages of mild reaction conditions, wide raw material source, low cost, and simple operation, can be applied to industrial production, and has a high medical and economic value.

Description

Technical field [0001] The invention relates to the technical field of deep processing of fish products, in particular to a method for extracting sardine polypeptides. Background technique [0002] Hypertension is known as the "silent killer". There are no obvious symptoms in the early stage until a clinical crisis occurs, such as a heart attack and a ruptured cerebral blood vessel. In my country, the incidence of hypertension has reached 18.8%, and the number of people who die of hypertension each year in the world is higher than that of any other disease. The mortality of cardiovascular and cerebrovascular diseases caused by hypertension has been ranked as the mortality of all diseases. The first place. [0003] At present, the drugs used for the treatment of hypertension mainly include angiotensin converting enzyme (ACE) inhibitors such as captopril, fosinopril, enalapril, etc. Although the curative effect is very obvious, its effect on the kidney Toxic and side effects, as wel...

Claims

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Application Information

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IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 刘月伟
Owner 广西钦州市绿源天然食品加工有限公司
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