Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Acid phosphatase staining method for distinguishing malignant serosal cavity effusion mesothelial cells from cancer cells and application thereof

A serosal cavity effusion and acid phosphatase technology, applied in the field of acid phosphatase staining, can solve the problems of difficult cancer cell differentiation, pathologist’s inability to make judgments, and low sensitivity of exfoliated cytology examination, etc. Convenience, reduced workload, guaranteed specificity and sensitivity

Inactive Publication Date: 2016-03-23
HEFEI JINCIKANG BIOTECH CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cytological examination makes judgments based on the morphological characteristics of the cells, and the mesothelial cells in the serosal cavity have various shapes due to hyperplasia, degeneration and other reasons, and it is not easy to distinguish them from cancer cells only by Papanicolaou staining. Exfoliative cytology of effusions often has low sensitivity (40%-60%), and inexperienced pathologists cannot even make a judgment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Acid phosphatase staining method for distinguishing malignant serosal cavity effusion mesothelial cells from cancer cells and application thereof
  • Acid phosphatase staining method for distinguishing malignant serosal cavity effusion mesothelial cells from cancer cells and application thereof
  • Acid phosphatase staining method for distinguishing malignant serosal cavity effusion mesothelial cells from cancer cells and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] 1. Acid Phosphatase Staining Kit Components:

[0013] (1) Fixative: 5-10% formalin

[0014] (2) Staining solution: 5-20g / l naphthol AS-BI phosphate dissolved in dimethylformamide

[0015] (3) Fuchsin hydrochloride: 2-10% fuchsin hydrochloride

[0016] (4) Sodium nitrite: 0.1-0.4MNaNO2

[0017] (5) Sodium acetate solution: 2-5M, pH4.5-5.5

[0018] (6) Distilled water

[0019] (7) Nuclear counterstaining solution: 2.5g of hematoxylin, 20ml of absolute alcohol, 5g of potassium aluminum sulfate, 330ml of distilled water, 250mg of sodium iodate, 150ml of glycerol, and 10ml of glacial acetic acid.

[0020] In addition, the above kit may also include an enzyme-labeled liquid-based cytology preservation solution, and its specific composition is as follows:

[0021] (1) 15% Methanol

[0022] (2) 0.1mM EDTA

[0023] (3) 0.1mM NaCl

[0024] (4) 0.01M sodium acetate buffer, pH5.2

[0025] (5) 5% Proclin300

[0026] (6)ddH 2 O.

[0027] 2. Dyeing steps:

[0028] (1) Cel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the field of auxiliary diagnosis, in particular to an acid phosphatase staining method for distinguishing malignant serosal cavity effusion mesothelial cells from cancer cells, a relevant reagent and the application of the method. By the adoption of the method, erythrocytes in a sample can be removed, and background influence can be eliminated; meanwhile, mesothelial cells and malignant cells can be distinguished distinctly, a doctor is helped to find malignant cells quickly and accurately and make a diagnosis on this basis, and diagnosis accuracy is improved greatly.

Description

technical field [0001] The invention relates to the field of auxiliary diagnosis, in particular to an acid phosphatase staining method for distinguishing malignant serous cavity effusion mesothelial cells from cancer cells, related reagents and applications thereof. Background technique [0002] The serous cavity is the gap between the serous parietal layer and the visceral layer, and is divided into the pleural cavity, peritoneal cavity, and pericardial cavity. There is a little serous fluid in the cavity, which acts as a lubricant, and it can increase in pathological conditions, forming pleural effusion, ascites, pericardial effusion, etc. The serosal effusions submitted for clinical examination are all pathological, and the cellular components in the serosal effusion include mesothelial cells, blood cells of non-epithelial origin, and tumor cells may also exist. Benign lesions (such as pneumonia, pulmonary infarction, influenza, liver abscess, tuberculosis, heart failure...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N1/30
Inventor 吴广平王阳方圣云方圣文
Owner HEFEI JINCIKANG BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com