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Rapid detection method for grain fungal pollution cases and applications thereof

A detection method and food technology are applied in the field of infrared spectroscopy to detect microorganisms, which can solve the problems of excessive time-consuming, complicated process of mold contamination, and high cost, and achieve the effects of short time-consuming, low cost, and easy operation.

Active Publication Date: 2016-03-23
NANJING UNIV OF FINANCE & ECONOMICS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the problems of complex process, long time-consuming or high cost in the existing methods for detecting mold contamination in grains, and to provide a method that can rapidly qualitatively and roughly quantify mold contamination in grains

Method used

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  • Rapid detection method for grain fungal pollution cases and applications thereof
  • Rapid detection method for grain fungal pollution cases and applications thereof
  • Rapid detection method for grain fungal pollution cases and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The infrared spectrum detection of seven kinds of fungal spore suspensions of embodiment 1

[0034] 1. Culture of mold

[0035] Cultured on potato dextrose agar (PDA). Fill a 500mL triangular flask with 400mL potato dextrose agar medium, sterilize at 121°C for 20 minutes, and when the medium cools down to about 55°C, pour the medium into a sterilized disposable petri dish, and wait for the After the culture medium was solidified, Aspergillus aureus 1, Aspergillus leucobacterium, Penicillium chrysogenum, Penicillium expanses, Fusarium oxysporum 1, Fusarium oxysporum 2, and Fusarium oxysporum 3 were inoculated on the PDA medium respectively, and the Cultured in an incubator at a temperature of 28°C.

[0036] 2. Harvesting and Processing of Spore Suspension

[0037] Take out the mold after culturing for 5 days, put 4mL sterilized aqueous glycerol solution (glycerol to water volume ratio is 1:10) on the surface of the culture medium, and after slight shaking, transfer th...

Embodiment 2

[0053] Qualitative and quantitative detection of contaminating Aspergillus gray in rice in embodiment 2

[0054] 1. Preparation of paddy samples containing Aspergillus glazee: Inoculate Aspergillus glazee 1 onto aseptic paddy sterilized by irradiation and cultivate at 28°C, take out the cultivated rice ten days after inoculation, and use a high-speed universal pulverizer ( Model FW100, Tianjin Test Instrument Co., Ltd.) was crushed for later use; at the same time, the aseptic paddy paddy sterilized by irradiation was pulverized as a control paddy sample. Add 2g, 5g, 10g, 20g and 50g of the above-mentioned rice powder containing Aspergillus aeruginosa to the aseptic rice respectively, and mix them uniformly to prepare rice samples containing different concentrations of Aspergillus aeruginosa. The total mass of each rice sample Both were 50g, and counted by traditional PDA medium culture, the number of Aspergillus aeruginosa contaminated in rice samples of each concentration was...

Embodiment 3

[0070] Example 3 Detection and Research of Polluted Different Molds in Paddy

[0071] Preparation of rice samples containing Aspergillus glazee: Inoculate Aspergillus glazee 1, Aspergillus glazee 2 and a strain of Penicillium on irradiated aseptic rice and cultivate them at 28°C, and take out the cultured samples ten days after inoculation. Paddy was pulverized into powder with a high-speed universal pulverizer (model FW100, Tianjin Test Instrument Co., Ltd.); at the same time, the aseptic paddy sterilized by irradiation was pulverized as a control paddy sample. Add rice powders of Aspergillus genus 1, 2 and a strain of Penicillium to the aseptic rice, and mix them evenly to prepare rice samples containing different molds. According to the traditional culture counting of the culture medium, the amount of bacteria in the rice contaminated by each mold is as follows: the amount of bacteria in the rice contaminated by Aspergillus grisea 2 is 1300cfu / g; the amount of bacteria in t...

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Abstract

The invention provides a rapid detection method for grain fungal pollution cases and applications thereof and relates to the microbiological detection field. The method comprises the following steps: grains polluted with control fungi and grains without fungi are mixed uniformly, and grain samples polluted with different control fungi or grain samples polluted with control fungi with different concentrations are obtained; the Fourier transform attenuation total reflection infrared spectrometer is employed to collect the infrared spectrogram and data of each polluted sample in characteristic bands; the infrared spectrometer data of each sample is subjected to principal component analysis, the principal component value is extracted, fungal discriminant models with different types or different concentrations are established by utilization of linear discriminant analysis or partial least square discriminant analysis, and the reliability of the discriminant models is verified; an infrared spectrogram and data of a grain sample to be detected in the characteristic bands are collected, and the model is employed to determine the type or the concentration of the polluted fungi in the grain sample to be detected. The method can carry out qualitative analysis and rough quantification of polluted mould in grains rapidly.

Description

technical field [0001] The invention relates to a method for detecting microorganisms by using infrared spectrum, a method for rapidly detecting fungus contamination of grain and an application thereof. Background technique [0002] Grain is very easy to mold and deteriorate under suitable conditions, which not only causes economic losses, but also the secondary metabolites of most toxin-producing molds seriously threaten the health of humans and animals. At present, the detection methods of mold contamination in grain mainly include: plate colony counting method, high performance liquid chromatography, gas chromatography and mass spectrometry, PCR and immunoassay, etc. The disadvantages of high cost and long time consumption make it difficult to monitor the growth of mold in grain in real time. Therefore, there is an urgent need to develop a detection method that can quickly identify contaminating molds in grains. Contents of the invention [0003] The purpose of the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35
CPCG01N21/3563
Inventor 都立辉刘凌平和肖营沈飞袁建鞠兴荣
Owner NANJING UNIV OF FINANCE & ECONOMICS
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