A preparation method of screen printing sensor for simultaneous detection of tetracycline and oxytetracycline
A technology of screen printing and oxytetracycline, which is applied in the direction of material electrochemical variables, etc., to achieve the effect of increasing the detection amount, increasing the loading site, and ensuring the accuracy
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Embodiment 1
[0029] A preparation method of screen printing sensor for simultaneous detection of tetracycline and oxytetracycline
[0030] (1) Add 3 µL of hydrochloric acid with a mass fraction of 0.015% dropwise on the working electrode of the dual-channel screen-printed electrode for 1 min, and wash it with ultrapure water;
[0031] (2) Add 3 µL, 0.1 mg / mL ZnO nanorod solution dropwise, and dry at room temperature;
[0032] (3) Add 3 µL, 0.1 mol / L EDC / NHS solution dropwise, incubate at room temperature for 0.2 h, wash with ultrapure water, and dry at room temperature;
[0033] (4) Add 3 µL, 0.1 µmol / L carboxyl- and ferrocene-modified tetracycline aptamer and 3 µL, 0.1 µmol / L carboxyl- and ferrocene-modified oxytetracycline aptamer to the dual-channel screen, respectively On the two working electrodes of the printed electrode, incubate at room temperature for 0.2 h, wash with ultrapure water, and dry at room temperature;
[0034] (5) Drop 3 µL, 0.01-20 ng / mL of a series of tetracycline ...
Embodiment 2
[0036] A preparation method of screen printing sensor for simultaneous detection of tetracycline and oxytetracycline
[0037] (1) Add 5 µL of hydrochloric acid with a mass fraction of 0.08% dropwise on the working electrode of the dual-channel screen-printed electrode for 10 minutes, and wash it with ultrapure water;
[0038] (2) Add 5 µL, 0.5 mg / mL ZnO nanorod solution dropwise, and dry at room temperature;
[0039] (3) Add 5 µL, 0.2 mol / L EDC / NHS solution dropwise, incubate at room temperature for 0.8 h, wash with ultrapure water, and dry at room temperature;
[0040] (4) Add 5 µL, 6 µmol / L carboxy- and ferrocene-modified tetracycline aptamer and 5 µL, 5 µmol / L carboxyl- and ferrocene-modified oxytetracycline aptamer to the double-channel filaments, respectively On the two working electrodes of the screen-printed electrode, incubate at room temperature for 0.7 h, wash with ultrapure water, and dry at room temperature;
[0041] (5) Drop 3 µL, 0.01-20 ng / mL of a series of te...
Embodiment 3
[0043] A preparation method of screen printing sensor for simultaneous detection of tetracycline and oxytetracycline
[0044] (1) Add 6 µL of hydrochloric acid with a mass fraction of 0.15% dropwise on the working electrode of the dual-channel screen-printed electrode for 30 minutes, and wash it with ultrapure water;
[0045] (2) Add 6 µL, 1.5 mg / mL zinc oxide nanorod solution dropwise, and dry at room temperature;
[0046] (3) Add 6 µL, 0.4 mol / L EDC / NHS solution dropwise, incubate at room temperature for 2 h, wash with ultrapure water, and dry at room temperature;
[0047] (4) Add 6 µL, 10 µmol / L of carboxyl- and ferrocene-modified tetracycline aptamer and 6 µL, 10 µmol / L carboxyl- and ferrocene-modified oxytetracycline aptamer to the double-channel filament, respectively On the two working electrodes of the screen-printed electrode, incubate at room temperature for 2 h, wash with ultrapure water, and dry at room temperature;
[0048] (5) Drop 6 µL, 0.01-20 ng / mL of a seri...
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