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Quorum-sensing inhibitor of aquatic Pseudomonas fluorescens and preparation method thereof

A technology of Pseudomonas fluorescens and quorum sensing, which is applied in the preservation, application, and food ingredients of food ingredients as antimicrobials, and achieves the effects of simple preparation method, reduced spoilage ability, and rich sources.

Active Publication Date: 2016-03-30
BOHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no public report on the application of Dan cortex extract in the aspect of quorum sensing inhibitor of Pseudomonas fluorescens in aquatic products.

Method used

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  • Quorum-sensing inhibitor of aquatic Pseudomonas fluorescens and preparation method thereof
  • Quorum-sensing inhibitor of aquatic Pseudomonas fluorescens and preparation method thereof
  • Quorum-sensing inhibitor of aquatic Pseudomonas fluorescens and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Dried paeonol in a constant temperature incubator at 30-40°C, crushed into powder to obtain paeonol powder, mixed the paeonol powder with 75% (W / V) methanol solution according to a material-to-liquid ratio of 1:8, First ultrasonic extraction for 10 minutes, then vibration extraction for 6 hours, the ultrasonic power of the ultrasonic extraction is 200-300W, the conditions of the vibration extraction are: temperature 30-40°C, vibration speed 150-180r / min; then remove the residue by suction filtration, Collect the filtrate; obtain the obtained filtrate by rotary evaporation to obtain a crude extract; add absolute ethanol to the crude extract for alcohol precipitation, and the alcohol precipitation time is 8h, wherein the crude extract and the anhydrous The volume ratio of ethanol is 1:5; suction filtration is carried out after the alcohol precipitation is completed, the filter residue is removed, and the filtrate is subjected to rotary evaporation to obtain a paeonol extrac...

Embodiment 2

[0041] Dried paeonol in a 30-40°C constant temperature incubator, crushed into powder to obtain paeonol powder, mixed the paeonol powder with 75% (W / V) methanol solution according to the ratio of material to liquid at 1:10, First ultrasonic extraction for 15 minutes, then vibration extraction for 3 hours, the ultrasonic power of the ultrasonic extraction is 200-300W, the conditions of the vibration extraction are: temperature 30-40°C, vibration speed 150-180r / min; then remove the residue by suction filtration, Collect the filtrate; obtain the obtained filtrate by rotary evaporation to obtain a crude extract; add absolute ethanol to the crude extract for alcohol precipitation, and the alcohol precipitation time is 8h, wherein the crude extract and the anhydrous The volume ratio of ethanol is 1:10; suction filtration is carried out after the alcohol precipitation is completed, the filter residue is removed, and the filtrate is subjected to rotary evaporation to obtain a paeonol e...

Embodiment 3

[0044] The preparation method of the paeonol extract used in this embodiment is the same as in Example 1, and it is used as the fresh-keeping research of aquatic product-turtfish, and the specific research method is as follows:

[0045] Fresh turbot was crushed and frozen to death, the fish meat was taken out, washed with sterile water and blotted dry with filter paper, the sterile fish pieces were respectively added to the prepared Pseudomonas fluorescens bacteria solution (control group) and Pseudomonas fluorescens Soak in bacterial solution + paeonol extract for 1min (paeonol extract treatment group), take it out immediately, blot dry with filter paper, sub-package, store at 4°C, measure the total number of bacterial colonies, pH value, TVB-N value and TBA of fish meat value.

[0046] Depend on figure 2 It can be seen that there was no significant difference in the total number of bacterial colonies between the paeonol extract treatment group and the control group, indica...

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Abstract

The invention relates to a quorum-sensing inhibitor of aquatic Pseudomonas fluorescens and a preparation method thereof. The quorum-sensing inhibitor can be used for development of compound preservatives for aquatic products. The preparation method comprises the following steps: taking moutan barks as the raw material; pre-treating the moutan barks; adding organic solvent into the pre-treated moutan barks, extracting by ultrasonic-assisted vibration, filtrating then collecting the filtrate; performing rotary evaporation to the filtrate, so that a crude extract is obtained; adding absolute ethyl alcohol into the crude extract to perform alcohol precipitation; performing suction filtration to remove the filter residues then performing rotary evaporation to the filtrate, so that the moutan-bark extract solution is obtained; finally, freezing and drying the moutan-bark extract solution, so that the moutan-bark extract is obtained. The moutan-bark extract is the quorum-sensing inhibitor. The invention is characterized in that the raw-material source is abundant and the preparation method is simple. The moutan-bark extract disclosed by the invention is capable of obstructing the quorum-sensing system of the aquatic Pseudomonas fluorescens and blocking the expression of the decay factors, so that the moutan-bark extract can be used for aquatic product preservation and in compound preservatives.

Description

technical field [0001] The invention belongs to the technical field of food storage and preservation, and in particular relates to a quorum sensing inhibitor of Pseudomonas fluorescens in aquatic products and a preparation method thereof. Background technique [0002] Fresh aquatic products are rich in nutrients and water, the muscle tissue is fragile, the content of soluble protein is high, and the pH value is close to neutral. However, fresh aquatic products are prone to corruption without effective fresh-keeping treatment. The main causes of spoilage of aquatic products include the action of enzymes, microorganisms and fat oxidation. Among them, microorganisms grow and reproduce by decomposing nutrients such as proteins, lipids, and sugars, causing corruption of aquatic products, resulting in huge economic losses and serious public health problems. The spoilage of aquatic products is not caused by all microbial activities, but specific spoilage bacteria play a key role....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B4/20
CPCA23B4/20A23V2002/00A23V2200/10A23V2250/02A23V2250/022A23V2250/1614A23V2250/21A23V2250/2132A23V2250/511A23V2300/10A23V2300/14A23V2300/48
Inventor 励建荣陈桂芳李学鹏仪淑敏李婷婷李钰金王彦波孙力军王雅玲
Owner BOHAI UNIV
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