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Platinum nanocage immune probe and preparation method thereof and application thereof in preparing electrochemical immunosensor

A platinum nanometer and probe technology is applied to platinum nanocage immunoprobes and their preparation and application in the preparation of electrochemical immunosensors. Uniform size effect

Inactive Publication Date: 2016-03-30
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current electrochemical detection mainly focuses on the detection of a single tumor marker, but due to the poor sensitivity and specificity between a single cancer marker and cancer, no cancer marker can become a " ideal” cancer screening tool

Method used

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  • Platinum nanocage immune probe and preparation method thereof and application thereof in preparing electrochemical immunosensor
  • Platinum nanocage immune probe and preparation method thereof and application thereof in preparing electrochemical immunosensor
  • Platinum nanocage immune probe and preparation method thereof and application thereof in preparing electrochemical immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Preparation of platinum nanocage particles

[0038] (1) 25mL of 12.5mM CTAB aqueous solution was stirred and heated to 95°C. After 5 minutes, 6.25mM, 1mL of chloropalladium acid aqueous solution was added, and after another 5 minutes, 0.1M, 0.1mL of ascorbic acid was quickly added, and after 20 minutes of reaction , centrifuged, and the precipitation was washed with deionized water and dispersed in 25mL deionized water to obtain a solution containing palladium nanoparticles; the morphology of the palladium nanoparticles obtained by transmission electron microscope observation was as follows: figure 1 As shown in A and 1C, it can be clearly seen that the palladium nanoparticles present a cubic morphology with a size of about 20 nm;

[0039] (2) Mix 6mL of the solution in step (1) with 21mL of 10mM CTAB, heat to 90°C, then mix 5mM, 3mL of K at a speed of 1mL / min 2 PtCl 4 Add it, react for 15 hours, centrifuge the product, and wash the precipitate with deionized water t...

Embodiment 2

[0055] 1. Preparation of platinum nanocage particles

[0056] (1) Stir 25mL of 10mM CTAB aqueous solution, heat to 80°C, after 5 minutes, add 1mM, 1mL aqueous solution of chloropalladium acid, and after another 5 minutes, quickly add 0.1M, 0.1mL ascorbic acid, react for 60 minutes, centrifuge, The precipitate was washed with deionized water, and then dispersed in 25mL of deionized water to obtain a solution containing palladium nanoparticles;

[0057] (2) 10mL of the solution obtained in step (1) was mixed with 21mL of 6mM CTAB, heated to 80°C, and then 0.1mM, 3mL of K 2 PtCl 4 Add it, react for 20 hours, centrifuge the product, wash with deionized water for precipitation, and obtain a solution containing platinum nanocage particles.

[0058] 2. Examples of immune probes prepared by using platinum nanocage particles and their immune properties:

[0059] 2.1 Tested sample: human serum

[0060] 2.2 Method

[0061] (1) Platinum nanocage particles were washed with PBS (pH5.0,...

Embodiment 3

[0072] 1. Preparation of platinum nanocage particles

[0073] (1) 25mL of 25mM CTAB aqueous solution was stirred and heated to 120°C. After 5 minutes, 10mM, 1mL of chloropalladium acid aqueous solution was added, and after another 5 minutes, 0.1M, 0.1mL of ascorbic acid was quickly added, reacted for 10 minutes, centrifuged, Wash with deionized water, and then disperse in 25mL deionized water to obtain a solution containing palladium nanoparticles;

[0074] (2) 10mL of the solution in step (1) was mixed with 21mL of 25mM CTAB, heated to 100°C, and then 10mM, 3mL of K 2 PtCl 4 Add it, react for 10 hours, centrifuge the product, wash the precipitate with deionized water, and obtain a solution containing platinum nanocage particles.

[0075] 2. Examples of immune probes prepared by using platinum nanocage particles and their immune properties:

[0076] 2.1 Tested sample: human serum

[0077] 2.2 Method

[0078] (1) Platinum nanocage particles were washed with PBS (pH8.0, KCl...

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Abstract

The invention discloses a platinum nanocage immune probe and a preparation method thereof and an application thereof in preparing electrochemical immunosensor. According to the invention, cubic platinum nanocage-shaped particles are obtained by substituting platinum atoms for palladium atoms in palladium nano cubic particles. The beneficial effects of the invention are: the method is simple; the obtained platinum nanocage-shaped particles are cubic in shape and have uniform sizes. Experimental results prove that, a cubic nanocage structure has a better catalysis effect than a globular platinum particle does so that the former has a more obvious signal amplification effect. An electrochemical immunosensor prepared by the immune probe which is prepared by platinum nanocage-shaped particles has strong and stable signals and good repeatability; simultaneous detection of double target is realized through marking electric signal material which can be distinguished by peak potential. The platinum nanocage immune probe provided in the invention and electrochemical immunosensors prepared with the same have a large application prospect in the field of immunosense.

Description

technical field [0001] The invention relates to a noble metal nanoparticle, its preparation method and its application in preparing immune probes, in particular to a platinum nanocage, its preparation method and its application in preparing immune probes and electrochemical immunosensors. Background technique [0002] Cancer is a deadly disease. Every year, 7 million people die from cancer worldwide, and 1 million people in my country lose their lives because of it. The likelihood of a cancer patient surviving depends largely on when the cancer was discovered. Early detection and treatment of cancer is the key to the survival of cancer patients. Cancer markers are signs of cancer occurrence, existence and development (Magdalena Swierczewska, Gang Liu, Seulki Lee, Xiaoyuan Chen, High-sensitivity nanosensors for biomarker detection, Chem. Soc. Rev., 2011, 41, 2641-2655.). Therefore, sensitive detection of cancer markers is a key factor for early detection and treatment of ca...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/00G01N33/574G01N27/26
Inventor 马占芳刘娜
Owner CAPITAL NORMAL UNIVERSITY
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