Primer probe system and kit for detection of mlh1 gene methylation
A technology for primer probes and detection primers, which is applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., can solve the problems of cumbersome operation, long operation time, high false positive rate, etc., and achieve elimination Effects of interference factors, guaranteed accuracy, and high sensitivity
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[0050] 1. The composition of the kit.
[0051] The MLH1 gene methylation detection kit of this embodiment includes mixed solution X, mixed solution Y, mixed solution Z and hot start enzyme (Taq Hotstar). Mixed solution X, mixed solution Y, mixed solution Z and hot start enzyme (Taq Hotstar) were placed in different test tubes, as shown in Table 1 to Table 3.
[0052] Table 1 Composition list of mixed solution X
[0053] components concentration Volume (μl) PCR buffer (PCRBuffer) 10× 1 dNTPs 2mM 1 MgCl 2
15mM 1 forward primer a 4μM 1 reverse primer a 4μM 1 Probe a 4μM 0.5 Pure water (ddH 2 O)
/ 1.5 total / 7
[0054] Table 2 Composition list of mixed liquid Y
[0055] components concentration Volume (μl) PCR buffer (PCRBuffer) 10× 1 dNTPs 2mM 1 MgCl 2
15mM 1 forward primer b 4μM 1 reverse primer b 4μM 1 probe b 4μM 0.5 Pur...
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