Kit and application thereof in genital tract pathogen proliferation testing

A kit and pathogen technology, applied in the field of microbial detection, can solve problems such as clinical diagnosis and treatment obstacles

Inactive Publication Date: 2016-03-30
天津华大基因科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since genital tract infections are not caused only by bacteria, this detection method will ignore the more important viral genital tract pathogens such as cytomegalovirus, hepatitis B virus, and herpes simplex virus, which will cause serious consequences for clinical diagnosis and treatment. certain obstacles

Method used

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  • Kit and application thereof in genital tract pathogen proliferation testing
  • Kit and application thereof in genital tract pathogen proliferation testing
  • Kit and application thereof in genital tract pathogen proliferation testing

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Experimental program
Comparison scheme
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specific Embodiment approach

[0030] According to a specific embodiment of the present invention, the detection method also includes:

[0031] (5) Determine whether the host is infected with certain pathogen(s).

[0032] Using the comparison results in (4), calculate the abundance of various pathogens contained in the sample to be tested, and compare the abundance of each pathogen with the non-pathogenic abundance of this pathogen. If there is a significant difference between the two, then Confirm that the host is infected with the pathogen. The abundance here reflects the amount of the pathogen in that sample. In a specific embodiment of the present invention, the determination of the non-pathogenic abundance of a certain pathogen, that is, the determination of the threshold, the threshold can be a specific value or a range of values ​​or even a formula, including: from multiple negative blood / Obtain multiple non-pathogenic abundances of a certain pathogen in plasma / serum samples, which can be obtain...

Embodiment

[0038] Patient urine / vaginal and cervical secretion samples (swab samples) came from Inner Mongolia Maternal and Child Health Hospital. IlluminaHiseq high-throughput sequencing technology was used to test whether the patient samples contained Ureaplasma urealyticum (UU), Neisseria gonorrhoeae (NG), group B streptococcus (GBS), Candida albicans (CA), vaginal Gardner bacteria (GV), Trichomonas vaginalis (TV), herpes simplex virus (HSV), Treponema pallidum (TP), Chlamydia trachomatis (CT), Mycoplasma genitalium (MG), Mycoplasma hominis (MH), Haemophilus dukei (HD) and / or cytomegalovirus (CMV) for detection, the operation steps are as follows:

[0039] 1. Primer Design

[0040] Firstly, according to the genome sequences of UU, NG, GBS, CA, GV, TV, HSV, TP, CT, MG, MH, HD, and CMV, PCR primer pairs were designed respectively, and multiple tests were screened to obtain the primers that can be used in the same PCR reaction system. Primer sequences for multiplex amplification.

[0...

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Abstract

The invention provides a kit capable of proliferating and / or testing pathogens and application thereof and a pathogen testing method and device. The kit comprises at least two pairs of the following 13 pairs of primer sequences: SEQ ID NO:1 and SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:6, SEQ ID NO:7 and SEQ ID NO:8, SEQ ID NO:9 and SEQ ID NO:10, SEQ ID NO:11 and SEQ ID NO:12, SEQ ID NO:13 and SEQ ID NO:14, SEQ ID NO:15 and SEQ ID NO:16, SEQ ID NO:17 and SEQ ID NO:18, SEQ ID NO:19 and SEQ ID NO:20, SEQ ID NO:21 and SEQ ID NO:22, SEQ ID NO:23 and SEQ ID NO:24 and SEQ ID NO:25 and SEQ ID NO:26. By utilizing the kit, the method and / or the device, multiple pathogens can be tested simultaneously, results are accurate, the detection cost is low, and consumed time is short.

Description

technical field [0001] The present invention relates to microorganism detection, in particular, the present invention relates to a kit comprising a sequence, a pathogen detection method and a device. Background technique [0002] In 2000, the Ministry of Health conducted an epidemiological survey on gynecological diseases among married women in some cities in 15 provinces, which showed that the prevalence of reproductive tract infections was the highest among gynecological diseases, and 42.9% of married women suffered from at least one genital tract infection. 19% of patients with genital tract infection had two or more mixed infections. Chronic cervicitis had the highest prevalence, 39.3%; bacterial vaginosis (BV), 5.3%; candida vaginitis (VVC), 4.8%; reproductive tract CT infection, 4.4%; pelvic inflammatory disease, 4.1%; Trichomonal vaginitis, 2.6%. And genital tract pathogen infection can cause the following diseases: 1) urinary system infection, pelvic inflammatory d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12Q1/14C12Q1/04C12M1/34C12R1/93C12R1/36C12R1/725C12R1/35C12R1/21C12R1/46C12R1/01
Inventor 宫艳萍吴红龙倪培相孙瑞雪李慧
Owner 天津华大基因科技有限公司
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