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Method for quickly detecting clenbuterol based on functionalized gold nanoparticles

A gold nanoparticle, functionalized technology, applied in material analysis by observing the influence of chemical indicators, color/spectral property measurement, and analysis by chemical reaction of materials, etc., can solve the complex and time-consuming detection process. Long, complex pork composition and other issues, to achieve the effect of wide application value, simple and convenient operation, simple real-time detection

Active Publication Date: 2016-03-30
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the residue of clenbuterol in the liver of animals is relatively high, and it will cause nausea, vomiting, muscle tremors, palpitation, trembling, headache, nausea, vomiting and other symptoms after consumption, especially for high blood pressure, heart disease, hyperthyroidism and Patients with diseases such as prostatic hypertrophy are more harmful, and severe cases can lead to death
[0004] The detection method of existing clenbuterol (clenbuterol hydrochloride) mainly is chromatographic method and immunoassay etc., although these methods are high in sensitivity, detection process is complicated, time-consuming is long; If use electrochemical method, although stability and The repeatability is good, but because of the complex components of pork and blood, it has caused serious interference to the detection of clenbuterol (clenbuterol hydrochloride); in the literature, there is a fluorescence method for colorimetric detection of clenbuterol, but due to the colorimetry of the fluorescence method The detection solution synthesis process is complicated and the repeatability is poor
It is difficult to achieve fast and timely on-site detection of clenbuterol (clenbuterol hydrochloride) in aqueous solution or blood, but the demand for this technology is particularly urgent

Method used

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  • Method for quickly detecting clenbuterol based on functionalized gold nanoparticles
  • Method for quickly detecting clenbuterol based on functionalized gold nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Preparation of gold nanoparticles modified by mercaptoethylamine-reduced sodium citrate

[0028] (a) Preparation of gold nanoparticles, 90mL1mM chloroauric acid solution is placed in a round-bottomed flask, heated and stirred until boiling, quickly added 10.8mL of sodium citrate solution with a mass fraction of 1%, and continued heating and stirring until the color of the solution changes from light to light After the yellow color becomes colorless, and finally turns into wine red, stop heating and stirring, let it stand to cool to room temperature, and store it in a refrigerator at 4°C.

[0029] (b) Take 20 mL of the gold nanoparticle aqueous solution prepared in (a), add 20 mL of 0.5 μM mercaptoethylamine solution dropwise under stirring, and continue stirring for 10 minutes to obtain a mercaptoethylamine-modified gold nanoparticle solution , and as a detection solution.

[0030] (2) Detection of Clenbuterol (Clenbuterol Hydrochloride) in Solution

[0031] (a) M...

Embodiment 2

[0034] (1) Preparation of gold nanoparticles modified by mercaptoethylamine-reduced sodium borohydride

[0035] (a) Preparation of gold nanoparticles, 4mL5mM chloroauric acid was added to 94mL ultrapure water, under the condition of rapid stirring, 2mL0.1M newly configured sodium borohydride was added dropwise, and after 20min of reaction, it could be prepared A wine-red gold nanoparticle solution was obtained.

[0036] (b) Take 20 mL of the gold nanoparticle solution prepared in (a), add 20 mL of 0.5 μM mercaptoethylamine solution dropwise under stirring, and continue stirring for 10 minutes to obtain a mercaptoethylamine-modified gold nanoparticle solution , and as a detection solution.

[0037] (2) Detection of clenbuterol (clenbuterol hydrochloride) in the solution.

[0038] (a) Measure two parts of equal-volume identical gold nanoparticle solutions in the detection solution prepared in step (1); prepare an aqueous solution that does not contain clenbuterol (clenbuterol ...

Embodiment 3

[0041] (1) Preparation of gold nanoparticles modified by mercaptoethylamine-reduced potassium citrate

[0042] (a) Preparation of gold nanoparticles, 90mL1mM chloroauric acid solution is placed in a round-bottomed flask, heated and stirred until boiling, quickly added 10.8mL of potassium citrate solution with a mass fraction of 1%, and continued heating and stirring until the color of the solution changes from light After the yellow color becomes colorless, and finally turns into wine red, stop heating and stirring, let it stand to cool to room temperature, and store it in a refrigerator at 4°C.

[0043] (b) Take 20 mL of the gold nanoparticle solution prepared in (a), add 20 mL of 0.5 μM mercaptoethylamine solution dropwise under stirring, and continue stirring for 20 minutes to obtain a mercaptoethylamine-modified gold nanoparticle solution , and as a detection solution.

[0044] (2) Detection of Clenbuterol (Clenbuterol Hydrochloride) in Solution

[0045] (a) Measure two ...

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Abstract

The invention discloses a method for quickly detecting clenbuterol based on functionalized gold nanoparticles. In the method, clenbuterol can generate hydrogen-bond interaction with sulfhydryl compound-modified gold nanoparticles to cause the resonance change of plasmas on the surfaces of the gold nanoparticles and the change of the color as well as ultraviolet-visible absorption strength and peak value of the gold nanoparticle solution, and therefore, whether a solution contains clenbuterol can be quickly detected by directly observing the color change of the solution with naked eyes. The method disclosed by the invention has the advantages of simplicity in operation, low cost, high sensitivity, wide application range and the like. The nano material can be used for realizing real-time and quick detection of clenbuterol.

Description

technical field [0001] The invention relates to a detection technology for clenbuterol (clenbuterol hydrochloride), in particular to a detection method for quickly and simply identifying low-concentration clenbuterol (clenbuterol hydrochloride) in an aqueous solution with complex components. Background technique [0002] "The country is based on the people, the people regard food as the first, and food safety is the first." In recent years, food safety incidents have occurred frequently, and consumers' health has been seriously threatened. Among them, since 2001, the Guangdong clenbuterol incident caused the poisoning of hundreds of people, making clenbuterol come into the attention of people. Subsequently, clenbuterol poisoning incidents occurred in Taiwan, Shanghai and many other places, especially the "Shuanghui" clenbuterol incident in 2011 has attracted widespread attention. [0003] Clenbuterol is a neurostimulant of the adrenal glands. It is used in pigs because it c...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/31G01N21/78
Inventor 李星吴爱国康晶燕张玉杰
Owner NINGBO UNIV
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