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Compound water purifying agent used for aquaculture and preparation method thereof

An aquaculture and water purifying agent technology, applied in water/sewage treatment, chemical instruments and methods, biological water/sewage treatment, etc., can solve problems such as the death of water animals, excessive reproduction of algae to form algal blooms or red tides, etc., to reduce Non-ionic ammonia concentration, detoxification and water quality improvement, overcoming slow onset effects

Active Publication Date: 2016-04-13
山东宝来利来生物工程股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, due to eutrophication, algal blooms or red tides will be formed due to eutrophication in the aquaculture water, which will cause a large number of deaths of water animals.

Method used

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  • Compound water purifying agent used for aquaculture and preparation method thereof
  • Compound water purifying agent used for aquaculture and preparation method thereof
  • Compound water purifying agent used for aquaculture and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Screening of bacterial strains with the ability to reduce ammonia nitrogen

[0030] 1 Materials and methods

[0031] 1.1 Test material

[0032] Strains: 111 strains of Bacillus were provided by Shandong Baolai Lilai Bioengineering Co., Ltd. Institute of Biology.

[0033] Bacillus culture medium: glucose 0.2%, peptone 1%, sodium chloride 0.5%, yeast extract 0.5%, pH7.0.

[0034] Breeding wastewater: fish farming wastewater, add NH 4 Cl20mg / L, NaNO 2 10mg / L.

[0035] 1.2 Test method:

[0036] (1) Determination of ammonia nitrogen - Nessler's reagent colorimetric method

[0037] (2) Determination of nitrite - α-naphthylamine colorimetric method

[0038] (3) Cultivation of Bacillus

[0039] 1.3 Screen the strains that have the effect of degrading ammonia nitrogen:

[0040] Proceed as follows:

[0041] (1) Filtrate the breeding wastewater, pack it into 500mL triangular flasks, 200mL per bottle, and sterilize it for later use.

[0042] (2) Insert the bacil...

Embodiment 2

[0054] Embodiment 2: the preparation of composite water purifying agent for aquaculture

[0055] 1 Test material:

[0056] (1) Screening strains:

[0057] The Bacillus subtilis B7348 and Bacillus subtilis N9-1-35 screened out in Example 1 of the present invention have the ability to degrade ammonia nitrogen.

[0058] (2) Compound strains:

[0059] Bacillus cereus with acid-producing ability and Bacillus subtilis BLS1 with algae-lytic ability.

[0060] (3) Physical and chemical reagents: zeolite powder, sodium thiosulfate, potassium aluminum sulfate.

[0061] 2 preparation method:

[0062] (1) Bacillus subtilis B7348, Bacillus subtilis N9-1-35, Bacillus cereus and Bacillus subtilis BLS1 were mixed in a mass ratio of 1:1:1:1 to prepare Microbial powder, the number of viable bacteria in the mixed microbial powder is not less than 10 8 cfu / g;

[0063] (2) Zeolite powder, sodium thiosulfate and potassium aluminum sulfate are mixed in a mass ratio of 4:3:2 to make a physical ...

Embodiment 3

[0065] Example 3: Verification of the water purification effect of the water purifier

[0066] 1.1 Test material:

[0067] (1) The composite water purifying agent prepared in Example 2 of the present invention;

[0068] (2) Bacterial agents: Bacillus subtilis B7348, Bacillus subtilis N9-1-35, Bacillus cereus and Bacillus subtilis BLS1 mixed in a mass ratio of 1:1:1:1 made;

[0069] (3) Physical and chemical reagents: Zeolite powder, sodium thiosulfate and potassium aluminum sulfate are mixed in a mass ratio of 4:3:2.

[0070] (4) Breeding wastewater: fish farming wastewater, add NH 4 Cl10mg / L, NaNO 2 2mg / L.

[0071] 1.2 Determination method:

[0072] (1) Determination of ammonia nitrogen - Nessler's reagent colorimetric method;

[0073] (2) Determination of nitrite - α-naphthylamine colorimetric method;

[0074] (3) Determination of pH value - pH meter;

[0075] 1.3 Test steps:

[0076] Prepare 10L of fish farming wastewater into aquaculture wastewater containing 10m...

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Abstract

The invention discloses a compound water purifying agent used for aquaculture. The compound water purifying agent is prepared by mixing microorganism bacterium powder with a physical and chemical reagent in a mass ratio of (1-3) to 1, wherein the microorganism bacterium powder is prepared by mixing bacillus subtilis B7348 with ammonia nitrogen degrading capacity, bacillus subtilis N9-1-35 with ammonia nitrogen degrading capacity, bacillus cereus with acid production capacity and bacillus subtilis BLS1 with algae lysing capacity; the number of viable bacteria in the microorganism bacterium powder obtained after mixing is more than or equal to 10<8>cfu / g; the physical and chemical reagent is prepared by mixing zeolite powder, sodium thiosulfate and aluminium potassium sulfate in a mass ratio of (3-5) to (2-4) to (1-3). The compound water purifying agent has good water purifying effects, is low in use cost, is safe and effective and can meet the requirements of mass aquaculture farmers.

Description

technical field [0001] The invention relates to a composite water purifying agent for aquaculture and a preparation method thereof, belonging to the technical field of aquaculture wastewater purification treatment. Background technique [0002] my country's aquaculture is currently adopting large-scale and intensive aquaculture, which consumes a lot of water resources, and the residual bait and animal excrement produced in the aquaculture process lead to eutrophication of the water body. The water environment has caused serious pollution, and the decline in the water quality of the aquaculture water has brought serious consequences and great economic losses to the aquaculture economy. [0003] Ammonia nitrogen, nitrite nitrogen, organic matter and suspended matter in aquaculture are the focus of water treatment. Ammonia nitrogen in water as non-ionic ammonia (NH 3 ) and ionic ammonium (NH 4+ ) form, non-ionic ammonia is the main factor causing aquatic organisms poisoning, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
CPCC02F1/281C02F1/5245C02F3/34C02F2101/16C02F2101/166C02F2101/30C02F2103/007
Inventor 单宝龙陈雷谷巍赵倩王红陈玉春高绪娜郭婷婷彭新利王志杰刘旭雅王春凤
Owner 山东宝来利来生物工程股份有限公司
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