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Method for cultivating bifidobacteria through inulin

A bifidobacterium and inulin technology, applied in the field of bifidobacteria cultivation, can solve the problems of poor activity, difficulty in preservation, and low content of bifidobacteria, and achieve the effects of increasing viscosity, increasing work pressure, and accelerating the pace of life

Inactive Publication Date: 2016-04-13
王军
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AI Technical Summary

Problems solved by technology

After more than 100 years, bifidobacteria have been widely used, but the content of bifidobacteria is low, the activity is poor, and it is difficult to preserve

Method used

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Embodiment 1

[0013] A method for cultivating bifidobacteria using inulin, the specific operation steps are: firstly carry out stock liquid culture, put the bifidobacterium strains into an anaerobic culture vessel for cultivation, and place the cultured bifidobacteria in the culture medium. Sterilized culture medium, the medium is composed of plant peptone, tryptone, yeast extract, glucose, saline solution, distilled water, and the culture vessel is placed in a ventilated place during cultivation to ensure that the culture vessel is treated in a low temperature state; then multi-stage bacteria The multi-level culture is divided into first-level static culture, dynamic culture, and second-level static culture. During the first-level static culture, potatoes and inulin are cooked, and after cooking, they are stirred and mixed together, and then the Put it into the culture medium as a culture medium for standby, and then inoculate the bacteria in the above steps into the culture medium; take th...

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Abstract

The invention provides a method for cultivating bifidobacteria through inulin. The method comprises specific operation steps as follows: cultivation of a stock culture liquid, cultivation of multiple stages of strains and solid culture. A traditional culture medium is changed, inulin is used for cultivation and contains a large amount of synanthrin, and synanthrin can prevent constipation, treat obesity, reduce lingering time of food in stomachs and intestines, increase faeces and effectively treat constipation; synanthrin can improve the viscosity of content and reduce the movement speed of food from the stomach to the intestines, accordingly, the sense of hunger is reduced, the food consumption is reduced, and an anti-obesity effect is performed, inulin is used for cultivating bifidobacteria, bifidobacteria absorbs synanthrin in the cultivation process, so that the activity and the absorption effect of bifidobacteria can be effectively improved, lactic acid and acetic acid can be generated after bifidobacteria are fermented in the intestines of a human body, the utilization rate of calcium, phosphorus and iron can be increased, and absorption of iron and vitamin D is promoted.

Description

technical field [0001] The invention relates to the field of bifidobacterium cultivation, in particular to a method for cultivating bifidobacteria by using inulin. Background technique [0002] Bifidobacterium has multiple functions such as anti-tumor, anti-aging, and lowering blood pressure, especially for gastrointestinal diseases. It can effectively block the source of gastrointestinal diseases, quickly kill pathogenic bacteria, enhance gastrointestinal function, and improve gastrointestinal immunity force. After more than 100 years, bifidobacteria have been widely used, but the content of bifidobacteria is low, the activity is poor, and it is difficult to preserve. Contents of the invention [0003] According to above technical problem, the present invention provides a kind of method utilizing inulin to carry out bifidobacterium cultivation, and its specific operation steps are: [0004] (1) First, the original seed liquid culture is carried out, and the bifidobacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 王军
Owner 王军
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