Probe, reagent kit and method for detecting bacteria in sputum

Abstract

The invention provides a probe for detection and particularly relates to a probe, reagent kit and method for detecting bacteria in a sputum sample. The reagent kit comprises a liquefaction solution, a lysis solution, a probe solution, a stop solution, an immobilization solution, slide glass and cover glass. According to the probe, reagent kit and method for detecting bacteria in the sputum sample, after the sputum sample is liquefied, cell lysis, probe hybridization, fluorescence immobilization and the like are performed, and bacteria are observed by naked eyes, counted, analyzed and the like through a fluorescence microscope. The reagent kit is simple in operation method, brief in program and easy and convenient to operate, the detection result is strong in specificity, high in sensitivity, clear and high in credibility, and the probe, the reagent kit and the method can be used for directly, easily, conveniently and effectively detecting bacteria in the sputum sample.

Description

technical field [0001] The invention relates to a probe for detection, in particular to a probe, kit and method for detecting bacteria in sputum samples. Background technique [0002] Bacterial culture and smear examination are one of the most widely used etiological diagnostic techniques, of which sputum culture accounts for more than 50%. The examination of sputum samples has important clinical significance for the diagnosis and differentiation of respiratory diseases. The lower respiratory tract of normal people is sterile. When bacterial infection occurs in the lungs or bronchi of the human body, the amount of sputum secretion will increase significantly. The pathogenic bacteria can be isolated through sputum bacterial culture, which is helpful for the diagnosis and treatment of lower respiratory tract infectious diseases. Common pathogenic bacteria found by sputum culture: [0003] Gram-positive bacteria: Streptococcus pneumoniae, Staphylococcus aureus, Mycobacterium...

AI Technical Summary

Problems solved by technology

In addition to the non-standard collection of sputum specimens, the culture results of unqualified specimens do not match the patient's infection, and the correlation between microorganisms and the human body is inherently complicated: the dialectical nature of pathogenicity from bacteria and conditional pathogenicity For example, pathogenic bacteria may be carried normally, and bacteria may simply colonize or infect the lower respiratory tract of patients with abnormal pulmonary ventilation function. It is a difficult problem in medical microbiology to prove the correlation between culture results and infection, which also makes Progress in the standardization of sputum culture has been slow

[0007] The positive rate of sputum bacterial culture is generally low, and the key factor for the poor clinical coincidence rate is that the collection of sputum samples is not standardized. Unqualified sputum samples will directly reduce the chance of pathogenic bacteria being isolated, and a large number of normal flora will also inhibit the growth or interference of pathogenic bacteria The detection of pathogenic bacteria reduces the clinical coincidence rate; what’s more, the operating procedures are not standardized, direct smears are not paid attention to, and the inspection process is not strictly controlled; and there are many types of bacteria in sputum, and their physiological characteristics and required Different culture conditions

If you want to use the culture method to detect the type and quantity of bacteria, you must use different culture media and culture conditions, and the workload is very heavy. All of the above are important reasons for the low clinical compliance rate.

[0008] Therefore, under the condition that the current sputum bacteria detection method cannot meet the requirements of simple and rapid detection of sputum bacteria samples, a detection method with wide application, low cost, easy operation, intuitive result judgment and high sensitivity is ready to be developed.

Images