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A compound and its application

A compound, methanol technology, applied in applications, microorganisms, biocides, etc., can solve problems such as unseen activity and reports of natural sources

Active Publication Date: 2018-10-30
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Literature searches at home and abroad show that 3-methoxycyclobutane 1,2-dione is used as a precursor for organic synthesis, but there are no reports on the activity and natural sources, and there are public literature reports on the application of nematicide preparations

Method used

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  • A compound and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: The poisonous action of compound to nematode

[0031] Dissolve 0.5 mg of 3-methoxycyclobutane 1,2-dione in methanol and dilute with sterile distilled water to obtain a 0.5 mg / ml solution. The final concentration of methanol does not exceed 5%.

[0032] The drug efficacy test was carried out according to the above-mentioned test method. Tables show adjusted mortality rates.

[0033] Table 1 The toxic effect of 0.5mg / ml 3-methoxycyclobutane 1,2-dione on M.incognita (%)

[0034]

[0035] The results show that the lethality of 3-methoxycyclobutane 1,2-diketone 0.5 mg / ml methanol solution to M.incognita nematodes reaches more than 50% in 12 hours, and reaches more than 60% in 36 hours, which has obvious nematode killing effect active.

Embodiment 2

[0036] Example 2: Compounds to the semi-lethal rate ED of nematodes 50

[0037] Example 2 The method is the same as that of Example 1, except that 2.5 mg of 3-methoxycyclobutane 1,2-diketone is dissolved in methanol to prepare a 1.0 mg / ml aqueous solution, which is then half-diluted to measure the ED 50 (half-lethal rate), the final concentration of methanol in the solution to be tested is no more than 5%.

[0038] Table 2 ED of 3-methoxycyclobutane 1,2-dione on M.incognita nematode 5

[0039]

[0040] The results showed that 3-methoxycyclobutane 1,2-dione on the ED of M.incognita nematode 50 (half-lethal rate, 24hr) was 0.447mg / ml.

Embodiment 3

[0041] Embodiment 3: Compound and emulsifier are mixed to the poisonous action of nematode

[0042] The method of Example 3 is the same as that of Example 1, except that 0.5 mg of 3-methoxycyclobutane 1,2-dione is dissolved in methanol, and then diluted with 0.3% Tween-20 aqueous solution, and the dilution concentration is 500 ppm , to obtain an emulsion with nematicide active substances. In the emulsion, the final concentration of methanol does not exceed 5%, and the final concentration of Tween-20 does not exceed 0.3%.

[0043] Table 3 Toxic effect of 3-methoxycyclobutane 1,2-diketone emulsified preparation on M.incognita

[0044]

[0045] The results show that the mixing of 3-methoxycyclobutane 1,2-dione and the emulsifier can increase the activity of killing M.incognita nematodes, and the increase rate is 10-20%.

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Abstract

The invention relates to a compound and an application thereof and belongs to the technical field of microorganism containing biopesticides. The compound is prepared according to steps as follows: a ZKB4-4 strain with the preservation number being CGMCC No.6351 is subjected to test tube slant seed culture, liquid culture, mass culture in a fermentation tank and chromatographic separation. The compound, namely, 3-methoxy-cyclobutane 1,3-diketone, is applied to preparation of a preparation for poisoning root-knot nematodes. 0.5 mg of the compound, namely, 3-methoxy-cyclobutane 1,3-diketone, is dissolved in methanol and diluted with a Tween-20 water solution, and an emulsion with the final concentration of methanol not higher than 5% and the final concentration of Tween-20 not higher than 0.3% is obtained and applied to preparation of the preparation for poisoning the root-knot nematode. Compared with existing pesticides for killing plant-parasitic nematodes, the compound has the advantages of high efficiency, low toxicity and low residues.

Description

Technical field: [0001] The invention relates to a compound and its application, and belongs to the technical field of microbial biological pesticides. Background technique: [0002] Plant root-knot nematode is a serious hazard. It not only brings serious diseases to humans and animals, but also causes losses equivalent to 104.4 billion US dollars to crops every year. At present, the control of nematodes is still dominated by chemical control, but most of the chemical nematicides are highly toxic and high-residual pesticides, which pose a serious threat to human resources and the environment. Some chemical nematicides, such as methyl bromide used as soil fumigants, have been banned or have stipulated a deadline for use in most countries around the world. With the enhancement of people's environmental awareness, the application of chemical nematicides is limited, so the research on the biocontrol of root-knot nematode naturally becomes the focus and focus. In the biocontrol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/26A01N35/06A01P5/00C12R1/01
CPCA01N35/06C12P7/26
Inventor 莫明和刘亚君吴凡亮李静张克勤
Owner YUNNAN UNIV
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