Listeria monocytogenes selective medium

Abstract

The invention discloses a listeria monocytogenes selective medium, and belongs to the field of detection of microculture. The listeria monocytogenes selective medium is characterized in that the prepared 1000 ml culture medium comprises the following components: yeast extract powder, beef powder, casein peptone, agar, glucose, sodium chloride, zinc gluconate, sodium dihydrogen phosphate, optical, polymyxin,bacitracin, gamma-arginine hydroxy and sterile horse blood protein, and distilled water is added into the components to 1000 ml. Compared with the prior art, the selective medium has the characteristics of being reliable in detection result, and high in listeria monocytogenes separation rate.

Description

technical field [0001] The invention relates to the field of test microorganism culture, in particular to a selective culture medium for Listeria monocytogenes. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.), using this medium can Separation of desired microorganisms from confounding microorganisms. Listeria monocytogenes is a facultative anaerobic bacterium widely distributed in the natural environment, such as soil, sewage, feed, animals, healthy people and various raw and ready-to-eat foods, including raw and cooked meat Products, quick-frozen rice noodles, cheese, vegetables, salads and seafood, etc., can cause listeriosis in humans, usually due to people eating food contaminated by the bacteria. After infection, the main clinical manifestations are fever, upper r...

AI Technical Summary

Problems solved by technology

At present, the gold standard for the identification of this genus is PCR identification, but PCR detection equipment requirements and operating costs are high, so trypticase soybean agar (TSA-YE) is often used for clinical culture.

However, the positive rate of Listeria monocytogenes culture is low, mainly because the bacteria are easily considered as contaminated bacteria (diphtheria-like bacteria) during the clinical culture process and discarded, and Escherichia coli, diphtheria-like bacteria, etc. The current medium grows faster than Listeria monocytogenes, and soon covers the medium, and Listeria monocytogenes cannot grow, which delays the time of diagnosis and treatment