Listeria monocytogenes selective medium

A technology for selecting medium and Listeria monocytogenes, which is applied in the field of selective medium producing Listeria monocytogenes, can solve the problems of high requirements and operating costs, discarding, and the inability to grow Listeria monocytogenes, and achieve the separation rate High and reliable detection effect

Inactive Publication Date: 2016-04-20
夏淑平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the gold standard for the identification of this genus is PCR identification, but PCR detection equipment requirements and operating costs are high, so trypticase soybean agar (TSA-YE) is often used for clinical culture.
However, the positive rate of Listeria monocytogenes culture is low, mainly because the bacteria are easily co

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1, the formula for preparing 1000ml medium contains: yeast extract powder 5g; beef powder 10g; casein peptone 6g; agar 15g; glucose 4g; sodium chloride 5g; sodium dihydrogen phosphate 1g; Zinc gluconate 0.01g; polymyxin 0.001g; bacitracin 0.003g; sterile equine blood protein 20g, add distilled water to 1000ml. Preparation method: Weigh yeast extract powder, beef powder, casein peptone, agar, glucose, sodium chloride, sodium dihydrogen phosphate, mix and dissolve in distilled water, sterilize at 210°C for 15 minutes, cool to 45°C, add sterilized gluconic acid Zinc; sterilized optical glutathione; sterilized polymyxin;

Embodiment 2

[0025] Example 2, the formula for preparing 1000ml medium contains: yeast extract powder 5g; beef powder 6g; casein peptone 8g; agar 15g; glucose 3g; sodium chloride 5g; sodium dihydrogen phosphate 3g; Zinc gluconate 0.05g; polymyxin 0.003g; bacitracin 0.001g; γ-hydroxyarginine 0.1g; Preparation method: Weigh yeast extract powder, beef powder, casein peptone, agar, glucose, sodium chloride, γ-hydroxyarginine, sodium dihydrogen phosphate, mix and dissolve in distilled water, sterilize at 210°C for 15 minutes, cool to 45 ℃, add sterilized zinc gluconate; sterilized optical glutathione; sterilized polymyxin;

Embodiment 3

[0026] Example 3, the formula for preparing 1000ml medium contains: yeast extract powder 5g; beef powder 8g; casein peptone 6g; agar 18g; glucose 5g; sodium chloride 5.5g; sodium dihydrogen phosphate 2g; Zinc gluconate 0.03g; Polymyxin 0.005g; Bacitracin 0.001g; Preparation method: Weigh yeast extract powder, beef powder, casein peptone, agar, glucose, sodium chloride, sodium dihydrogen phosphate, acelinin, mix and dissolve in distilled water, sterilize at 210°C for 15 minutes, cool to 45°C, add Sterilized zinc gluconate; sterilized optical glutathione; sterilized polymyxin;

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PUM

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Abstract

The invention discloses a listeria monocytogenes selective medium, and belongs to the field of detection of microculture. The listeria monocytogenes selective medium is characterized in that the prepared 1000 ml culture medium comprises the following components: yeast extract powder, beef powder, casein peptone, agar, glucose, sodium chloride, zinc gluconate, sodium dihydrogen phosphate, optical, polymyxin,bacitracin, gamma-arginine hydroxy and sterile horse blood protein, and distilled water is added into the components to 1000 ml. Compared with the prior art, the selective medium has the characteristics of being reliable in detection result, and high in listeria monocytogenes separation rate.

Description

technical field [0001] The invention relates to the field of test microorganism culture, in particular to a selective culture medium for Listeria monocytogenes. Background technique [0002] Microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective medium is a medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.), using this medium can Separation of desired microorganisms from confounding microorganisms. Listeria monocytogenes is a facultative anaerobic bacterium widely distributed in the natural environment, such as soil, sewage, feed, animals, healthy people and various raw and ready-to-eat foods, including raw and cooked meat Products, quick-frozen rice noodles, cheese, vegetables, salads and seafood, etc., can cause listeriosis in humans, usually due to people eating food contaminated by the bacteria. After infection, the main clinical manifestations are fever, upper r...

Claims

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Application Information

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IPC IPC(8): C12Q1/04
CPCC12Q1/045
Inventor 夏淑平唐志勐
Owner 夏淑平
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