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Method for producing acid by fermenting excess sludge and synthesizing microbial oil

A microbial oil and excess sludge technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low oil production, release and existing forms that are not suitable for yeast to use, and poor effects, etc., to achieve Low production cost and remarkable effect of promoting production

Active Publication Date: 2016-04-27
TONGJI UNIV
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Problems solved by technology

However, in these studies, the incomplete pretreatment resulted in insufficient carbon source for oleaginous yeast in the sludge. Therefore, the effect of cultivating oleaginous yeast with pure residual sludge as a substrate was poor, and additional carbon was needed. Sources and other main nutrients can obtain higher microbial oil production
[0004] Some studies have used conventional physical and chemical methods to pretreat excess sludge and then used it for the cultivation of oleaginous yeast. However, due to the release and existence of organic matter that is not suitable for yeast to use, the oil yield is low.

Method used

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  • Method for producing acid by fermenting excess sludge and synthesizing microbial oil

Examples

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Embodiment 1

[0032] (1) Anaerobic fermentation: Settling the remaining sludge at 4°C for 12-24 hours until the concentration of the concentrated sludge in the lower layer is between 15-20g / L, remove the supernatant, and take 300mL of concentrated sludge to 1L In an anaerobic fermentation tank with a volume of 0.1g / gTSS, add tea saponin at a concentration of 0.1g / gTSS, and carry out anaerobic fermentation of sludge under anaerobic conditions. The time is 7 days. The method of solid-liquid separation of the fermentation broth is centrifugation, the centrifugal force of the centrifugation is 10000×g, and the centrifugation time is 20 minutes to obtain the acid-producing supernatant.

[0033] (2) Removal of ammonia nitrogen in the supernatant: collect the acid-producing clear liquid obtained from the anaerobic fermentation of the sludge, and take an appropriate amount of the supernatant from the sludge fermentation, according to N:P:Mg(mol:mol:mol)=1:2.2: 2.2 Add a specified amount of disodiu...

Embodiment 2

[0044] (1) Anaerobic fermentation: Settling the remaining sludge at 4°C for 12-24 hours until the concentration of the concentrated sludge in the lower layer is between 15-20g / L, remove the supernatant, and take 300mL of concentrated sludge to 1L In an anaerobic fermentation tank with a volume of 0.1g / gTSS, add tea saponin at a concentration of 0.1g / gTSS, and carry out anaerobic fermentation of sludge under anaerobic conditions. The time is 7 days. The method of solid-liquid separation of the fermentation broth is centrifugation, the centrifugal force of the centrifugation is 10000×g, and the centrifugation time is 20 minutes to obtain the acid-producing supernatant.

[0045] (2) Removal of ammonia nitrogen in the supernatant: collect the acid-producing clear liquid obtained from the anaerobic fermentation of the sludge, take an appropriate amount of the supernatant from the sludge fermentation, and add it according to N:P:Mg(mol:mol)=1:2.2:2.2 A specified amount of disodium ...

Embodiment 3

[0056] (1) Anaerobic fermentation: Settling the remaining sludge at 4°C for 12-24 hours until the concentration of the concentrated sludge in the lower layer is between 15-20g / L, remove the supernatant, and take 300mL of concentrated sludge to 1L In an anaerobic fermentation tank with a volume of 0.1g / gTSS, add tea saponin at a concentration of 0.1g / gTSS, and carry out anaerobic fermentation of sludge under anaerobic conditions. The time is 7 days. The method of solid-liquid separation of the fermentation broth is centrifugation, the centrifugal force of the centrifugation is 10000×g, and the centrifugation time is 20 minutes to obtain the acid-producing supernatant.

[0057] (2) Removal of ammonia nitrogen in the supernatant: collect the acid-producing clear liquid obtained from the anaerobic fermentation of the sludge, take an appropriate amount of the supernatant from the sludge fermentation, and add it according to N:P:Mg(mol:mol)=1:2.2:2.2 A specified amount of disodium ...

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Abstract

The invention relates to a method for producing acid by fermenting excess sludge and synthesizing microbial oil, belonging to the field of resourceful treatment of sludge. The method comprises the following steps: (1) taking the excess sludge as a raw material, adding tea saponin into the excess sludge, and carrying out anaerobic fermentation to obtain a supernate; (2) removing ammonia nitrogen in the supernate obtained in the step (1) by adopting a struvite precipitation method, thus obtaining a solution containing volatile fatty acids; (3) by taking the solution obtained in the step (2) as a culture medium, culturing an oil-producing yeast by adopting a sequencing batch culturing method; and (4) by taking the yeast cells obtained after the culturing in the step (3) as a raw material, and extracting to obtain the microbial oil. Compared with the prior art, the low-concentration volatile fatty acids generated by the excess sludge are adopted as the culture medium for synthesizing the microbial oil with high content, and therefore, the environmental benefit and economic benefit brought by application of the excess sludge for producing acid are improved.

Description

technical field [0001] The invention relates to a method for treating excess sludge, in particular to a method for producing acid by fermentation of excess sludge and synthesizing microbial oil, which belongs to the field of sludge resource treatment and also relates to the field of microbial oil synthesis. Background technique [0002] Excess sludge is the main by-product of urban sewage treatment plants. Its treatment and disposal costs are high and improper treatment will have serious adverse effects on the environment. On the other hand, excess sludge contains a large amount of organic matter and other nutrients for microbial growth. It has potential resource utilization value. The use of excess sludge to produce biodiesel is actually to obtain the raw material that can synthesize biodiesel from excess sludge—grease, which is further processed and converted into a new renewable energy source—biodiesel. There are two main ways to obtain oil from excess sludge: one is to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 刘佳黄翔峰刘佳楠苑明陈锐陆丽君彭开铭
Owner TONGJI UNIV
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