Bioremediation preparation of aniline contaminated water

A bioremediation and preparation technology, applied in the field of microorganisms, can solve problems such as abnormal operation of sewage treatment equipment, and achieve the effects of improving biological treatment efficiency, easy method, and fast reproduction

Active Publication Date: 2016-05-04
徐州如山环保生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many chemical plants have used degradative microorganisms to treat aniline compounds in industrial wastewater, and have achieved remarkable results, but they still cannot fully meet the actual needs. A c

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A bioremediation preparation for aniline-polluted water bodies, the preparation includes the following raw materials: a composite bacterial agent and auxiliary materials, the composite bacterial agent is composed of Pseudomonas Preparation of nitrifying bacteria, Trichoderma chrysogenum, and nitrifying bacteria.

[0031] The auxiliary material is formed by mixing diatomite and peat soil according to a weight ratio of 3:1;

[0032] The composite bacterial agent and auxiliary materials are mixed according to a weight ratio of 2:5 to prepare a bioremediation preparation;

[0033] The preparation method of the composite bacterial agent is as follows: respectively culture Pseudomonas putida, Candida, Bacillus amyloliquefaciens, Clostridium papyrisolubles, nitrosative bacteria, Trichoderma chrysanthemum, and nitrifying bacteria to a concentration of 1×10 8 bacterium solution per ml, and then mixed according to the volume ratio of 5:2:3:1:4:3:2 to obtain;

[0034] Described Ps...

Embodiment 2

[0044] The method of using the bioremediation preparation prepared in Example 1: put the bioremediation preparation into the aniline-contaminated water body, wherein the weight ratio of the bioremediation preparation to the water body is 1:700, wherein the aniline-contaminated water body is artificially prepared, and the aniline initial The concentration is 50 mg / L, and then shake culture at room temperature for 24 hours, the concentration of aniline drops to 0.01 mg / L, and the degradation rate reaches 99.9%.

Embodiment 3

[0046] The bioremediation preparation prepared in Example 1 was used to treat the industrial sewage of a chemical plant. The concentration of COD in the sewage was 670 mg / L, the concentration of dinitrotoluene + trinitrotoluene was 179 mg / L, and the concentration of aniline was 70 mg / L. Add 20 grams of the preparation prepared in Example 1 to cubic meters of sewage each time, and add once a day. After 10 days of continuous addition, the treatment effect detected is as follows: the concentration of effluent COD is 12 mg / L, and the concentration of nitrobenzenes is Below 1mg / L, the concentration of anilines is 0.01mg / L. The specific results are shown in Table 1:

[0047] Table 1

[0048]

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PUM

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Abstract

The invention relates to a bioremediation preparation of aniline contaminated water. The bioremediation preparation is prepared from a compound bacterium agent and auxiliary materials. The compound bacterium agent is prepared from pseudomonas putida, candida mycoderma bacteria, bacillus amyloliquefaciens, clostridium, nitrosomonas, trichoderma aureoviride and nitrifying bacteria. The strains are reasonable in compatibility, coexist and coordinate with one another, and are not mutually antagonistic. If the preparation is put into a wastewater treatment system, a good degradation effect is achieved on aniline degradation-resistant compounds, the preparation is suitable for industrial sewage treatment, the amount of treated water is increased, the quality of the treated water is improved, operation cost is reduced, and up-to-standard discharge is promoted.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bioremediation preparation for aniline polluted water bodies. Background technique [0002] Aniline is the most representative substance of aromatic amines. It is a colorless oily liquid with aromatic odor. It is widely used in national defense, printing and dyeing, plastics, paint, pesticide and pharmaceutical industries, etc. It is also a serious environmental pollution and endangers human health. It is a kind of "carcinogenic, teratogenic and mutagenic" three substances. Due to the characteristics of long-term residue, bioaccumulation, and carcinogenicity, aniline is listed as 129 pollutants for priority control by the US EPA, and is also included in the "China Environmental Priority Pollutants Blacklist", which requires strict requirements in industrial drainage. control. The traditional methods for treating aniline wastewater mainly include physical, c...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C02F3/34C12R1/07C12R1/40C12R1/72C12R1/01C12R1/885C12R1/145C02F101/38
CPCC02F3/34C02F2101/38C12N1/14C12N1/20
Inventor 孙小春
Owner 徐州如山环保生物科技有限公司
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