Biological agent and method for preventing and controlling banana wilt

A technology for banana fusarium wilt and banana fusarium wilt is applied in the directions of botanical equipment and methods, biocides, chemicals for biological control, etc. The development of breeding work, etc., to achieve good results and inhibit the effect of development

Active Publication Date: 2016-05-11
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The developmental regulation mechanism of Fusarium wilt of banana itself is unknown, wh...

Method used

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  • Biological agent and method for preventing and controlling banana wilt
  • Biological agent and method for preventing and controlling banana wilt
  • Biological agent and method for preventing and controlling banana wilt

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Embodiment 1

[0040] So far, there is no effective method for the prevention and control of Fusarium wilt of banana. One important reason is that the pathogen has strong resistance. The early development of fungal spores is an important development process in its life history, and it is also the most critical link for its colonization on host plants. Most fungicides that have effective control effects on plant fungal diseases are aimed at the germination and early stages of fungal spores. of the developmental process. In view of this, the present invention identifies and verifies for the first time the key proteins and important metabolic pathways of the early development of Banana Fusarium wilt Tropical Race 4, and the research results can be used for the development of new fungicides and the induction of gene silencing in host plants to cultivate new disease-resistant species qualitative target sites.

[0041] 1. The present invention uses the latest "Shotgun"-based quantitative proteomi...

Embodiment 2

[0082] Application of embodiment 2 in cultivating resistant banana fusarium wilt transgenic banana new lines:

[0083] (1) Construction and genetic transformation of FocTR4ERG6 and FocTR4ERG11 double-stranded RNA overexpression vectors

[0084] The backbone of the overexpression vector is the pYL-RNAi vector (gifted by Liu Yaoguang, a researcher at the School of Life Sciences, South China Agricultural University), and on this basis, the FocTR4ERG6 and FocTR4ERG11 double-stranded RNA overexpression vectors pYL-Ubi-ERG6 and pYL-Ubi-ERG11 were constructed.

[0085]The construction steps of the pYL-Ubi-ERG6 overexpression vector are as follows: select specific fragments from the two coding genes (SEQ ID NO.1 and 2) of the FocTR4ERG6 protein, and artificially synthesize the tandem fragments. The sequence of the tandem fragments is shown in SEQ ID NO.6 , connected to the multiple cloning site 1 of the pYL-RNAi vector, and then the artificially synthesized tandem fragment was reverse...

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Abstract

The invention discloses a biological agent and a method for preventing and controlling banana wilt. The method comprises the following step: performing gene silencing regulation and control on genes or similar genes of relevant proteins of a banana wilt bacterium tropical No.4 physiological race ergosterol biosynthesis process. The active ingredients of the biological agent for preventing and controlling banana wilt include a double-stranded RNA interfere vector, an anti-sense RNA interfere vector or a microRNA interfere vector constructed by aiming at the genes or similar genes of relevant proteins of the banana wilt bacterium tropical No.4 physiological race ergosterol biosynthesis process, wherein the double-stranded RNA interfere vector, the anti-sense RNA interfere vector or the microRNA interfere vector is capable of causing target coding gene silencing. According to the invention, key protein and important metabolic pathways for the early development of banana wilt bacterium physiological race are identified and verified, and the research result can serve as a target site for development of new bactericides and disease-resistant new germplasm culture based on host plant virus-induced gene silencing.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a biological preparation and method for preventing and controlling banana wilt. Background technique [0002] Banana is a plant belonging to Monocotyledons, Zingiberaceae, Musaceae, and Musa genus. It is widely distributed in the world, mainly distributed in tropical and subtropical regions between north and south latitude 20°, and has a total of more than 130 countries and regions. The area grows bananas. Banana (Musaspp.) is not only an important economic crop but also an important food crop. It is the second largest fruit in the world after citrus; it is also the fourth largest food crop after rice, wheat and corn (Moffat, 1999; Huang Yonghong, 2011 ). Fusarium wilt caused by the fungus Fusarium oxysporum f.sp.cubense has exploded and spread in large areas around the world, which has seriously threatened the banana industry and become the main factor restricting its sustainable ...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01N63/02A01N63/04A01N57/16A01P3/00
CPCA01H1/06A01N57/16A01N63/10A01N63/30
Inventor 杨乔松易干军邓贵明窦同心丁利杰
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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