High performance liquid chromatographic method for rapid quantitative assessment of honey quality

Abstract

The invention discloses a high performance liquid chromatographic method for rapid quantitative assessment of honey quality. According to the method, 10 to 20 parts of a representative honey sample is collected, and a proper amount of the sample is weighed and pre-treated by using pure acetonitrile for high performance liquid chromatographic detection; a special analytical column for sugar is used as a chromatographic column, and acetonitrile-water (75: 25) is used as a mobile phase; a differential refraction detector is used for detection, an external standard method is used for quantification, and the contents of glucose, fructose and cane sugar are calculated. The method provided by the invention employs high performance liquid chromatography and the special analytical column for sugar to successively separate glucose, fructose and cane sugar in honey, acetonitrile-water (75: 25) is used as the mobile phase, and chromatographic separation is finished in about 15 min; the method has good separation effect, high determination repeatability, detection sensitivity and a recovery rate of 97.0 to 98.9% and is easy in pretreatment and operation; acetonitrile and water are used as solvents and acetonitrile and water can be recycled and are free of pollution, so clean production is realized; and the method protects the interests of consumers and is of guiding significance to production of honey production enterprises.

Description

technical field [0001] The invention relates to a chromatographic method for quantifying the quality of honey, in particular to a high-performance liquid chromatographic method for rapidly quantifying the quality of honey. Background technique [0002] Honey contains more than 180 active substances, the main components of which are fructose and glucose and 4% to 5% fructooligosaccharides. In addition, honey is also rich in amino acids, vitamins, active enzymes, flavonoids, polyphenols, and carotenoids. Vitamins and other active substances and a variety of minerals. Honey has a variety of physiological activities such as anti-oxidation, antibacterial, accelerated wound repair, and enhanced immunity. Therefore, honey is not only used as a direct and effective source of nutritional supplements, but also used to treat hepatitis, cancer, wound infection, constipation, and improve stomach. Intestinal function, improve the body's immunity, etc. [0003] In recent years, honey has...

AI Technical Summary

Problems solved by technology

However, ion chromatography has high requirements for reagents and injection samples, strict requirements for sample pretreatment steps, and requires special ion chromatography and pulsed amperometric detectors

Capillary electrophoresis, gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods are expensive and difficult to popularize in small and medium-sized honey production enterprises in China