High performance liquid chromatographic method for rapid quantitative assessment of honey quality

A high-performance liquid chromatography and honey technology, which is applied in the chromatographic field of quantitative honey quality, can solve the problems of difficult popularization, high requirements, expensive equipment, etc., and achieve the effects of sensitive detection, simple pretreatment and operation, and high repeatability

Inactive Publication Date: 2016-05-11
天津中发蜂业科技发展有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, ion chromatography has high requirements for reagents and injection samples, strict requirements for sample pretreatment steps, and requires special ion chromatography and pulsed amperometric detectors
Capillary electrophoresis, gas chromatography-mass spectrometry, nuclear magnetic resonance and other methods are expensive and difficult to popularize in small and medium-sized honey production enterprises in China

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1 A kind of high performance liquid chromatography method of quantitative honey quality rapidly

[0014] 1. Sample processing

[0015] Weigh about 100mg of fructose and glucose and about 20mg of sucrose respectively, weigh them accurately, put them in a 50ml measuring bottle, dissolve and dilute to the mark with mobile phase, and shake well.

[0016] Weigh 0.5g honey sample, add 2mL pure acetonitrile, 65℃ water bath for 10min, centrifuge at 10000r / min for 20min, draw the supernatant into a 25mL volumetric flask, add 3mL distilled water to the residue, 65℃ waterbath for 10min, centrifuge at 10000r / min, combine The supernatant was extracted again according to this method, and the supernatant was combined, and the volume was adjusted to 25 mL with distilled water, and 5 mL was extracted and filtered with an organic system (0.45 μm filter membrane) for detection by high performance liquid chromatography.

[0017] 2. Chromatographic analysis conditions

[0018] ...

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PUM

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Abstract

The invention discloses a high performance liquid chromatographic method for rapid quantitative assessment of honey quality. According to the method, 10 to 20 parts of a representative honey sample is collected, and a proper amount of the sample is weighed and pre-treated by using pure acetonitrile for high performance liquid chromatographic detection; a special analytical column for sugar is used as a chromatographic column, and acetonitrile-water (75: 25) is used as a mobile phase; a differential refraction detector is used for detection, an external standard method is used for quantification, and the contents of glucose, fructose and cane sugar are calculated. The method provided by the invention employs high performance liquid chromatography and the special analytical column for sugar to successively separate glucose, fructose and cane sugar in honey, acetonitrile-water (75: 25) is used as the mobile phase, and chromatographic separation is finished in about 15 min; the method has good separation effect, high determination repeatability, detection sensitivity and a recovery rate of 97.0 to 98.9% and is easy in pretreatment and operation; acetonitrile and water are used as solvents and acetonitrile and water can be recycled and are free of pollution, so clean production is realized; and the method protects the interests of consumers and is of guiding significance to production of honey production enterprises.

Description

technical field [0001] The invention relates to a chromatographic method for quantifying the quality of honey, in particular to a high-performance liquid chromatographic method for rapidly quantifying the quality of honey. Background technique [0002] Honey contains more than 180 active substances, the main components of which are fructose and glucose and 4% to 5% fructooligosaccharides. In addition, honey is also rich in amino acids, vitamins, active enzymes, flavonoids, polyphenols, and carotenoids. Vitamins and other active substances and a variety of minerals. Honey has a variety of physiological activities such as anti-oxidation, antibacterial, accelerated wound repair, and enhanced immunity. Therefore, honey is not only used as a direct and effective source of nutritional supplements, but also used to treat hepatitis, cancer, wound infection, constipation, and improve stomach. Intestinal function, improve the body's immunity, etc. [0003] In recent years, honey has...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 韩雪
Owner 天津中发蜂业科技发展有限公司
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