Biological material for improving release of endothelial progenitor cell exosomes (EPC-EXs) and promoting bone defect repair, and preparation method and application thereof

A technology of endothelial progenitor cells and biomaterials, applied in the field of regeneration and repair of bone injury, can solve problems such as lack of further research, and achieve the effect of promoting release, significant effect, and broad clinical application prospects.

Inactive Publication Date: 2016-05-18
WUHAN DAFU BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently...

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  • Biological material for improving release of endothelial progenitor cell exosomes (EPC-EXs) and promoting bone defect repair, and preparation method and application thereof
  • Biological material for improving release of endothelial progenitor cell exosomes (EPC-EXs) and promoting bone defect repair, and preparation method and application thereof

Examples

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Embodiment 1

[0016] Example 1: A biomaterial that improves the release of endothelial progenitor cell exosomes and promotes bone defect repair, characterized in that the biomaterial is in situ intermolecular cross-linked by chondroitin oxysulfate (CS) and type I collagen (ColI) The constructed polyelectrolyte multilayer membrane, in which the outermost component is CS; the assembly concentration of CS and ColI are 0.5mg / mL respectively; the oxidation degree of CS is 25%; the assembly conditions are: pH 4.0, ionic strength 0.15 mol / LNaCl, the temperature is room temperature.

[0017] The preparation method of the biological material that increases the release of endothelial progenitor cell exosomes and promotes bone defect repair is characterized in that it is carried out according to the following steps: 1. The culture plate is used as the assembly substrate, and the assembly substrate is first immersed in pH 7.4 at room temperature. In a polyethyleneimine (PEI) solution with a concentration...

Embodiment 2

[0019] The biological material for enhancing the release of endothelial progenitor cell exosomes and promoting bone defect repair, characterized in that the biological material is composed of oxidized chondroitin sulfate (CS) or oxidized hyaluronic acid (HA) and type I collagen (ColI) through in situ The polyelectrolyte multilayer membrane constructed by intermolecular cross-linking, in which the outermost component is CS; the assembly concentration of CS and ColI are 2mg / mL respectively; the oxidation degree of CS is 50%; the assembly conditions are: pH5.0, ionic strength It is 0.15mol / L NaCl and the temperature is room temperature.

[0020] The preparation method of the biological material that increases the release of endothelial progenitor cell exosomes and promotes bone defect repair is characterized in that it is carried out according to the following steps: 1. The culture plate is used as the assembly substrate, and the assembly substrate is first immersed in pH 7.4 at room...

Embodiment 3

[0022] A biomaterial for enhancing the release of endothelial progenitor cell exosomes and promoting bone defect repair, characterized in that the biomaterial is a polyelectrolyte constructed by in situ intermolecular cross-linking of oxidized chondroitin sulfate (CS) and type I collagen (ColI) Multilayer film, where the outermost component is CS; the assembly concentration of CS and ColI are 1.0 mg / mL respectively; the oxidation degree of CS is 40%; the assembly conditions are: pH 4.5, ionic strength 0.15 mol / L NaCl, temperature It is room temperature.

[0023] The preparation method of the biological material that increases the release of endothelial progenitor cell exosomes and promotes bone defect repair is characterized in that it is carried out according to the following steps: 1. The culture plate is used as the assembly substrate, and the assembly substrate is first immersed in pH 7.4 at room temperature. In a polyethyleneimine (PEI) solution with a concentration of 5mg / m...

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Abstract

The invention relates to a biological material for improving release of endothelial progenitor cell exosomes (EPC-EXs) and promoting bone defect repair, and a preparation method and application thereof. The biological material is a polyelectrolyte multilayer film constructed by carrying out in situ intermolecular crosslinking on oxidized chondroitin sulfate (CS) and type I collagen (Col I), wherein the component of the outermost layer is CS; the assembly concentration of each of CS and Col I is 0.5-2mg/mL; the oxidation degree of CS is 25-50%; the assembly conditions are that the pH value is 4.0-5.0 and the ionic strength is 0.15mol/L NaCl; and the temperature is room temperature. The biological material combines the advantages of a material and a cell, and can be widely applied to promotion of vascular regeneration and vascular repair of tissue damages. The angiogenesis is conductive to reducing tissue damages and promoting the repair of damaged bone tissues, so that EPCs and PEC-EXs with the capability of angiogenesis have broad clinical application prospects in bone injury repair.

Description

Technical field [0001] The invention relates to a biological material, a preparation method and application for improving the release of endothelial progenitor cell exosomes and promoting bone defect repair, and is used for the regeneration and repair of bone injury, belonging to the medical technology field. Background technique [0002] Exosomes (EXs) are a type of biologically active small vesicles (30-150nm in diameter) released during cell activation or apoptosis, which contain functional proteins, mRNAs and microRNAs (miRs), etc. Substances are an important medium for mutual communication and mutual influence between cells. The latest research found that stem cells secrete EXs as an important component that plays a key role in the process of tissue damage repair and regeneration. Under suitable conditions, the EXs secreted by stem cells can promote skeletal muscle regeneration by carrying certain miRs related to tissue regeneration (such as the ability of stem cells to res...

Claims

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Application Information

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IPC IPC(8): A61L27/40
CPCA61L27/16A61L27/20A61L27/24A61L2430/02C08L5/08C08L23/06
Inventor 陈颜芳赵名艳李章华
Owner WUHAN DAFU BIOTECH CO LTD
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