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Active additive for promoting agricultural waste compost maturity and application thereof

A technology of active additives and agricultural waste, applied in the direction of organic fertilizers, etc., can solve the problems of inability to degrade obviously

Inactive Publication Date: 2016-05-18
CHONGQING GUANTAI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the primary fermentation stage of compost, since it contains a large amount of easily degradable organic matter, the addition of pectin and lignocellulose-degrading bacteria at this stage cannot significantly degrade these substances

Method used

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  • Active additive for promoting agricultural waste compost maturity and application thereof
  • Active additive for promoting agricultural waste compost maturity and application thereof
  • Active additive for promoting agricultural waste compost maturity and application thereof

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Experimental program
Comparison scheme
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Embodiment Construction

[0021] 1. Seed culture: Bacillus subtilis (Bacillus subtilis), Aspergillus niger (Aspergillus niger), Trichodermaviride (Trichodermaviride), and Penicillium simplicissimum (Penicillium simplicissimum) were respectively inoculated from the slant medium to beef extract peptone medium, Chase medium, Wort juice medium and potato sucrose medium, 200ml of medium in a 1L Erlenmeyer flask, 37°C, 200rpm, cultured for 30h.

[0022] 2. Fermentation culture: Put 4L of fermentation medium in a 5L automatic fermenter, mix the seed culture solution at a ratio of 18%, 35%, 22% and 25%, and then inoculate the mixed seed solution at 8% into the fermenter , cultured at 37°C, 500rpm, 1.2vvm for 4d.

[0023] 3. The obtained fermented liquid is filtered with four layers of gauze to remove bacteria and solid matrix, which is the active additive. After determination, the active additive pectinase activity is 78.4U·ml -1 , xylanase activity is 65.9U·ml -1 , the filter paper cellulase activity is 42...

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Abstract

The invention relates to enzyme liquid treatment on agricultural waste compost. An active additive for promoting agricultural waste compost maturity is a composite enzyme liquid which is produced by mixing and fermenting bacillus, aspergillus niger, trichoderma and penicillium according to certain proportion. The fermentation liquid, after being filtered, is directly added to the compost by 0.5-1% by weight of compost raw materials when the agricultural waste compost begins. The active additive contains abundant pectinase and lingocellulase and can promote degradation on pectin, hemicelluloses, cellulose and the like in a primary fermentation step and break on cell wall tissue of plants, so that the microorganisms can attack the cellulose and lignin more easily during a high-temperature step and a decay step, thereby accelerating the compost maturity process and improving the quality of the compost.

Description

technical field [0001] The invention relates to solid waste treatment, in particular to an enzyme solution treatment for promoting the composting and maturity of agricultural waste. Background technique: [0002] my country is a large agricultural country, and a large amount of agricultural waste produced every year not only affects the environment, but also wastes resources. Aerobic composting is an important means of resourceful and harmless treatment of agricultural waste. Since agricultural waste, especially crop straw, is rich in refractory lignocellulose, it has problems such as long composting treatment cycle and low compost quality. Inoculation of microbial cells is currently a commonly used method to promote the composting process. In the invention patent of ZL03118137.6 "Twice Inoculation of Microbial Compound Bacteria Composting Method", it is proposed to add 1# compound Bacteria and 2# composite bacteria can exert their lignocellulose degradation ability and pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F11/08
Inventor 张彩鹏霍曼浩
Owner CHONGQING GUANTAI TECH CO LTD