Method for conducting fermentative production of citric acid by adding saccharifying enzyme stage by stage based on pH responses

A saccharification enzyme and citric acid technology, applied in the field of fermentation engineering, can solve problems such as the loss of saccharification enzyme activity, and achieve the effects of ensuring rapid growth, efficient utilization and shortening fermentation cycle

Inactive Publication Date: 2016-05-18
JIANGNAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for fermenting citric acid by adding glucoamylase in the pH response stage, which can solve the problem of loss of glucoamylase activity in the middle and later stages of citric acid fermentation, effectively shorten the fermentation cycle, increase the fermentation conversion rate, and reduce the final fermentation rate. residual sugar in fermentation broth

Method used

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  • Method for conducting fermentative production of citric acid by adding saccharifying enzyme stage by stage based on pH responses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Mix cornflour and water evenly at a ratio of 1:1.5, adjust the pH to 5.8, then add high-temperature α-amylase, the addition amount is 50U / g cornflour; two spray liquefaction, the temperature of the first spray is 105 ° C, the second The injection temperature is 130°C, and the iodine test is qualified to obtain a liquefied mixed liquid, and part of the liquefied mixed liquid is filtered through a plate frame to obtain a liquefied clear liquid; the liquefied mixed liquid is mixed with water evenly, and a certain amount of ammonium sulfate is added to prepare a seed medium (total sugar 6%, total Niger 0.25%), inoculate the seed medium with the mature Aspergillus niger spore suspension, increase the stirring speed to 800rpm when culturing for 8 hours, and co-cultivate for 27 hours to obtain mature seed liquid; mix the liquefied mixed liquid, liquefied clear liquid and water to prepare fermentation culture base (total sugar 16%, total nitrogen 0.05%); inoculate the mature see...

Embodiment 2

[0029] Mix cornflour and water evenly in a ratio of 1:4, adjust the pH to 6.0, then add high-temperature α-amylase, the addition amount is 15U / g cornflour; two sprays are liquefied, the temperature of the first spray is 95°C, and the second spray is liquefied. The injection temperature is 120°C, and the iodine test is qualified to obtain a liquefied mixed solution, and part of the liquefied mixed solution is filtered through a plate frame to obtain a liquefied clear solution; the liquefied mixed solution is mixed with water evenly, and a certain amount of ammonium sulfate is added to prepare a seed medium (total sugar 10%, total Niger 0.30%), the mature Aspergillus niger spore suspension was inoculated into the seed medium, cultivated for 26 hours, and the mature seed liquid was obtained; the liquefied mixed liquid, the liquefied clear liquid and water were mixed to prepare a fermentation medium (total sugar 16.8%, total nitrogen 0.08% ); Inoculate the mature seed liquid into t...

Embodiment 3

[0031] Mix cassava flour and water evenly at a ratio of 1:2.5, adjust the pH to 5.9, then add high-temperature α-amylase, the addition amount is 25U / g cassava flour; two sprays are liquefied, the temperature of the first spray is 100°C, and the second spray is liquefied. The injection temperature is 125°C, and the iodine test is qualified to obtain a liquefied mixed liquid, and part of the liquefied mixed liquid is filtered through a plate frame to obtain a liquefied clear liquid; the liquefied mixed liquid is mixed with water evenly, and a certain amount of ammonium sulfate is added to prepare a seed medium (total sugar 12%, total Niger 0.40%), the mature Aspergillus niger spore suspension was inoculated into the seed medium, cultivated for 30 hours, and the mature seed liquid was obtained; the liquefied mixed liquid, the liquefied clear liquid and water were mixed to prepare a fermentation medium (total sugar 16.7%, total nitrogen 0.12% ); inoculate the mature seed liquid int...

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Abstract

The invention discloses a method for conducting fermentative production of citric acid by adding a saccharifying enzyme stage by stage based on pH responses. The method includes the steps that a starchy material is subjected to pulp mixing, liquefaction and inoculated fermentation to obtain end fermentation liquor; when the pH of the fermentation liquor is reduced to 2.0-3.0 in the fermentative culture process, the saccharifying enzyme is added into the fermentation liquor stage by stage in an equivalent mode every 2-8 h, wherein the total addition quantity of the saccharifying enzyme is 40-1000 U / g residual non-glucose quantity. By means of the method, the problem of saccharifying enzyme activity loss caused in the middle-late stage of citric acid fermentation can be solved, the fermentation period can be effectively shortened, the fermentation conversion rate can be increased, the residual glucose quantity of the end fermentation liquor can be reduced, wastewater discharge can be reduced, and meanwhile the activity of glucosidase secreted by glucose can be offset to reduce non-fermentable glucose. The method has significant economic benefits and environmental benefits.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a method for producing citric acid by adding glucoamylase to ferment and produce citric acid based on the pH response stage. Background technique [0002] Citric acid is an important organic acid with multiple functions. It is widely used in food, medicine, chemical industry and other fields. It is the edible organic acid with the largest output and consumption in the world. The global output exceeds 1.7 million tons. At the same time, citric acid has excellent biological characteristics, and has great application potential in emerging industries such as biopolymerization, drug delivery, and cell culture, and its demand is growing at a rate of 5% per year. [0003] Citric acid is often obtained by liquid submerged fermentation, and the traditional batch fermentation method is still used in industrial production. Because Aspergillus niger has the adva...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/48C12P19/14C12R1/685
CPCC12P7/48C12P19/14
Inventor 石贵阳陈坚王宝石胡志杰蒋小东孙福新金赛谭凤玲张梁李由然
Owner JIANGNAN UNIV
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