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Harmless treatment method for antibiotic mycelium residues

A technology of antibiotic bacteria residue and harmless treatment, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the secondary pollution of dioxin or biogas slurry, increase secondary pollution, and waste high protein Resources and other issues, to achieve good environmental and economic benefits, low cost, and simple processing methods

Inactive Publication Date: 2016-06-01
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of antibiotic production and the gradual improvement of environmental protection requirements, the problem of bacterial residue treatment has become increasingly prominent. Some enterprises can only dry and seal the bacterial residue, which not only increases the occupied area, but also increases the possibility of secondary pollution and production costs. and environmental pressure greatly increased
Bacterial residue incineration or the use of sewage treatment systems or biogas fermentation can partially solve the problem of antibiotic pollution, but the equipment investment and operation costs are huge, and there is a risk of secondary pollution such as dioxins or biogas slurry
The existing treatment method also wastes the high-protein resources contained in the fungus residue, as long as the residual antibiotics contained in the potential environmental hazards are removed and the potential environmental risks are eliminated, the highly valuable high-protein resources can be prepared

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of polymyxin residue solid fermentation strain

[0030] Polymyxin residue 1000g

[0031] Glucose 30g

[0032] Grass Powder 35g

[0033] Microbial complex strains 10g

[0034] According to the above weight ratio, add glucose and grass powder to the polymyxin slag with a water content of 50%, add water and mix evenly, after mixing, the total water content is 50%, insert microbial compound strains, and ferment for 80hrs, The polymyxin residue solid fermentation strain is made.

[0035] 2. Harmless treatment of polymyxin residue

[0036] The polymyxin solid fermentation strain prepared in the above steps, the fresh polymyxin residue with a total water content of 50%, and the grass powder are mixed according to the weight ratio of 100:8:10, and the air is continuously supplied for oxygen supply. 96hr fermentation to obtain high-protein polymyxin harmless fermentation scum. During the fermentation process, the temperature was controlled at 33°C and the ai...

Embodiment 2

[0044] 1. Preparation of streptomycin residue solid fermentation strain

[0045] Streptomycin residue 1000g

[0046] Glucose 30g

[0047] Grass powder 70g

[0048] Microbial complex strains 18g

[0049] According to the above weight ratio, add glucose and grass powder to the fresh streptomycin residue with a water content of 60%, add water and mix, and mix to make a total water content of 60%, insert microbial composite strains, and ferment for 90hr , to make streptomycin residue solid fermentation strains.

[0050] 2. Harmless treatment of streptomycin residue

[0051] Mix the streptomycin solid fermentation strain prepared in the above steps, fresh streptomycin residue with a total water content of 60%, and grass powder in a weight ratio of 100:10:7, and continuously feed air for oxygen supply, and ferment for 96 hours , to obtain high-protein streptomycin harmless fermentation scum. During the fermentation process, the temperature was controlled at 30°C and the air hu...

Embodiment 3

[0058] 1. Preparation of oxytetracycline residue solid fermentation strain

[0059] Oxytetracycline residue 1000g

[0060] Glucose 30g

[0061] Grass Powder 35g

[0062] Microbial complex strains 25g

[0063] According to the above weight ratio, add glucose and grass powder to the oxytetracycline slag with a water content of 50%, add water and mix, the total water content after mixing is 50%, insert microbial composite strains, ferment for 85hr, and prepare A solid fermentation strain of oxytetracycline residue.

[0064] 2. Harmless treatment of oxytetracycline residue

[0065] Mix the oxytetracycline solid fermentation strain prepared in the above steps, fresh oxytetracycline residue with a total water content of 55% and grass powder in a weight ratio of 100:6:8, and continuously feed air for oxygen supply, and ferment for 90 hours , to obtain high-protein oxytetracycline harmless fermentation bacteria residue. During the fermentation process, the temperature was controll...

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Abstract

The invention relates to a harmless treatment method for antibiotic mycelium residues. The treatment method comprises the steps of firstly, adding glucose, a microbial compound strain and grass meal into the antibiotic mycelium residues, mixing uniformly, and performing culture to prepare a solid fermentation strain; and secondly, mixing the strain, the grass meal and fresh antibiotic mycelium residues according to a weight ratio, and fermenting for 72-96 hours to obtain harmlessly fermented high-protein antibiotic mycelium residues. A mycelium residue fermentation product is dried, ground and screened by a 60-mesh sieve to obtain fermented high-protein mycelium powder. With the method, no polluted water is generated in the process, the fermentation product is free of antibiotic residues through detection with HPLC (High Performance Liquid Chromatography) and cylinder plate methods, secondary harmless wastes are not generated, the protein content of the harmlessly treated high-protein mycelium powder exceeds 30%, the equipment investment and the running cost are low, the effects of energy conservation and environmental protection are achieved, and the prepared high-protein mycelium powder can be further applied as a resource and has high environmental and economic benefits.

Description

technical field [0001] The invention relates to the fields of antibiotic fermentation and environmental protection, in particular to a harmless treatment method for antibiotic residues. Background technique [0002] my country is the world's largest producer and exporter of antibiotic raw materials, with an annual output of more than 200,000 tons. It is estimated that 8 to 10 tons of antibiotic residues are produced for every ton of antibiotics produced, and more than one million tons of antibiotic residues are discharged every year. In February 2002, the Ministry of Agriculture, the Ministry of Health, and the State Drug Administration announced No. 176 to include antibiotic residues in the catalog of drugs that are prohibited from being used in feed and animal drinking water. The "National Hazardous Waste List" revised in 2008 also stipulates that antibiotic residues must be managed as hazardous waste. With the development of antibiotic production and the gradual improvem...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14C12R1/23C12R1/02C12R1/125C12R1/10C12R1/845C12R1/865C12R1/69C12R1/545C12R1/01C12R1/645
CPCC12N1/20C12N1/14C12N1/18
Inventor 王明兹吴嫣然汤丹芳刘欣吴钦缘陈必链
Owner FUJIAN NORMAL UNIV
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