Kasugamycin fermentation medium and fermentation method thereof

Abstract

The invention relates to a kasugamycin fermentation medium. The fermentation medium contains tween80 and an antifoaming agent, wherein the antifoaming agent is an organosilicon antifoaming agent and / or a polyether antifoaming agent. The kasugamycin fermentation medium substantially reduces the generation amount of foams in a liquid fermentation process through synergism of the tween80, the antifoaming gent and a medium raw material in order to improve the oxygen transmission efficiency and well avoid liquid overflow and bacterial infection caused by a large amount of foams. The fermentation medium and the culture method can substantially improve the fermentation output of kasugamycin.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a kasugamycin fermentation medium and a fermentation method. Background technique [0002] In the process of using microorganisms for fermentation and production, a large amount of foam may be generated in the culture system. The reasons for the foam can be roughly attributed to aeration and agitation, medium ratio, strain characteristics, inoculum size, and sterilization quality. The adverse effects of foam on fermentation include: the filling coefficient of the fermenter is reduced, the oxygen transfer efficiency is reduced, it is easy to cause liquid escape, and the increase in the chance of bacterial contamination leads to abnormal metabolism of the bacteria. Therefore, the amount of suppressing foam is the basic condition to ensure normal fermentation. Industry generally uses the fermentation medium that adds defoaming agent to suppress the production amount ...

AI Technical Summary

Problems solved by technology

The industry generally uses the fermentation medium that is added with defoaming agent to suppress the production amount of foam, and the inventor of the present invention has found in the practice of utilizing Streptomyces aureus strains to carry out kasugamycin fermentation production that existing defoaming agents are added The fermentation medium of agent (for example, ethylene oxide propylene oxide polymer, stearamide and emulsified silicone oil) is difficult to effectively suppress the generation of foam, which restricts the improvement of kasugamycin fermentation yield

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