Molecular imprinting-Raman combination detection method of sulfadimidine in milk

A technology of sulfamethazine and a detection method, which is applied to the molecular imprinting of sulfamethazine in milk - the field of general detection of sulfamethazine, can solve the problems of expensive instruments, long time consumption and high detection cost, and achieves reduction of detection cost and rapid process. convenient effects

Inactive Publication Date: 2016-06-01
河北伊诺光学科技股份有限公司
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Problems solved by technology

Although the existing method has the advantages of high sensitivity and low detection limit, it is difficult to meet the cur

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Embodiment Construction

[0013] In order to make the objects and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0014] The embodiment of the present invention provides a molecular imprinting-Raman detection method for sulfamethazine in milk, comprising the following steps:

[0015] S1. Preparation of sulfamethazine molecularly imprinted hydrogel: take 0.017g sulfamethazine (template molecule), 1.4ml acrylic acid (functional monomer), 3.0g acrylamide (functional monomer), 0.09g N'N- Methylenebisacrylamide (cross-linking agent), 0.09g ammonium persulfate (initiator) and 4.0ml deionized water (solvent) were fully mixed in a beaker, and after deoxygenation by nitrogen, it was filled to the In the splint space formed by the glass slide, place it in an oven at...

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Abstract

The invention discloses a molecular imprinting-Raman combination detection method of sulfadimidine in milk. The method can realize rapid low-cost detection of excess sulfadimidine in milk. A hydrogel prepared through a molecular imprinting technology is used to complete adsorption and enrichment of sulfadimidine in a detected sample in 5min, and a portable Raman spectrometer is used to collect spectrum information, so the whole process is rapid and convenient; and the hydrogel can be repeatedly used, so the detection cost is reduced, environmental protection is realized, and onsite high-flux detection requirements are met.

Description

technical field [0001] The invention relates to a detection method, in particular to a molecular imprint-Raman combined detection method of sulfamethazine in milk. Background technique [0002] As a commonly used sulfonamide antibacterial agent, sulfamethazine is widely used to prevent and treat bacterial infectious diseases in dairy cattle. Because of its long action time and metabolism time in animals, it is easy to remain in animal tissues and then enter animal secretions (such as milk). Long-term consumption of dairy products with high residues of sulfonamides will have serious consequences on the health of the human body, especially infants and young children. At present, the detection methods of sulfa drug residues in milk include high performance liquid chromatography (HPLC), gas chromatography (GC), gas-mass spectrometry (GC / MS), liquid-mass spectrometry (LC / MS), etc. . Although the existing methods have the advantages of high sensitivity and low detection limit, ...

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Application Information

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IPC IPC(8): G01N21/65
Inventor 陈达谭棕
Owner 河北伊诺光学科技股份有限公司
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