A Method for Collagen Self-Assembly Mediated by Fluid Shear Force
A fluid shear force and self-assembly technology, applied in the field of biomedical materials and biomechanics, can solve the problems of ignoring the mechanical microenvironment and not being able to reproduce the assembly process well, and achieve easy recycling and processing, simple preparation methods, Good biocompatibility and degradability effect
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Embodiment 1
[0024] Example 1: Collagen self-assembly mediated by fluid shear force
[0025] Add 400 μL of 10x PBS solution, 2.76 μL of 1M NaOH solution, and 3.477 mL of deionized water to the centrifuge tube in turn, mix well, and take out the type I rat tail collagen solution with a mass concentration of 3.34 mg / mL from the 4°C refrigerator , take 119.8 μL and add it to prepare a sample solution with a mass concentration of 0.10 mg / mL, and adjust the pH between 7.0 and 7.4 with diluted ammonia water. Take 3.9mL of the sample solution and drop it on the plate of the cone-plate viscometer, put down the cone-plate, set the fluid shear force to 0.5Pa, the action time is 1h, the reaction temperature is 25°C, and the force is applied. After the stress loading was completed, the suspension was collected, centrifuged at high speed and washed 3 times. The precipitate was frozen in a -20°C refrigerator for 5 hours, and then transferred to a vacuum freeze dryer for 12 hours to freeze-dry to obtain...
Embodiment 2
[0026] Example 2: Collagen self-assembly mediated by fluid shear force
[0027] Add 400 μL of 10x PBS solution, 2.76 μL of KOH solution with a molar concentration of 1M, and 3.477 mL of deionized water to the centrifuge tube, mix well, and take out the type I rat tail collagen solution with a mass concentration of 3.34 mg / mL from the 4°C refrigerator , take 119.8 μL and add it to prepare a sample solution with a mass concentration of 0.10 mg / mL, and adjust the pH between 7.0 and 7.4 with diluted sodium hydroxide solution. Take 3.9mL of the sample solution and drop it on the plate of the cone-plate viscometer, put down the cone-plate, set the fluid shear force to 0.5Pa, the action time is 1h, the reaction temperature is 25°C, and the force is applied. After the stress loading was completed, the suspension was collected, centrifuged at high speed and washed 3 times. The precipitate was frozen in a -20°C refrigerator for 5 hours, and then transferred to a vacuum freeze dryer for...
Embodiment 3
[0028] Example 3: Collagen self-assembly mediated by fluid shear force
[0029] Add 400 μL of 10x PBS solution, 2.76 μL of 1M NaOH solution, and 3.477 mL of deionized water to the centrifuge tube in turn, mix well, and take out the type I rat tail collagen solution with a mass concentration of 3.34 mg / mL from the 4°C refrigerator , take 119.8 μL and add it to prepare a sample solution with a mass concentration of 0.10 mg / mL, and adjust the pH between 7.0 and 7.4 with diluted ammonia water. Take 3.9mL of the sample solution and drop it on the plate of the cone-plate viscometer, put down the cone-plate, set the fluid shear force to 2.0Pa, the action time is 1h, the reaction temperature is 25°C, and the force is applied. After the stress loading was completed, the suspension was collected, centrifuged at high speed and washed 3 times. The precipitate was frozen in a -20°C refrigerator for 5 hours, and then transferred to a vacuum freeze dryer for 12 hours to freeze-dry to obtain...
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