Method for extracting beta-1,3-D-glucan from cell walls of fungi

A cell wall and glucan technology, applied in the biological field, can solve the problems of low efficiency and large alkali consumption, and achieve the effects of reducing cost, low alkali consumption and high extraction efficiency

Active Publication Date: 2016-06-08
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of large alkali consumption and low efficiency of the existing alkaline method, and provide an improved method for extracting β-1,3-D-glucan from the cell wall of fungi

Method used

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  • Method for extracting beta-1,3-D-glucan from cell walls of fungi

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0030] This preparation example is used to prepare the cell wall used in each example.

[0031] Brewer's yeast pretreatment: Use 200 mesh net cloth to screen waste beer yeast (dry matter content of 16% by weight, provided by Tianjin Snow Beer Co., Ltd.) to remove impurities, centrifuge at 2000×g for 10 minutes, and use cold saline for precipitation Wash twice to get clean beer yeast cells.

[0032] Preparation of the cell wall of brewer's yeast: pretreated clean brewer's yeast cells and distilled water to prepare a 50% by weight cell suspension, use a high-pressure homogeneous crusher under 1700bar conditions to break twice; 8341 × g centrifugation for 20 minutes, precipitation Washed with cold deionized water for 3 times and centrifuged to remove the supernatant to obtain the cell wall of beer yeast (water content of 82% by weight).

[0033] Take a large cup mushroom (purchased from China Forestry Microbial Culture Collection and Management Center, CFCC87186), prepare the cell wall...

Embodiment 1

[0035] Add 12g of brewer’s yeast cell wall to 48mL of 0.9g / 100mL NaOH solution to form a cell wall suspension, treat it at 108°C for 5min, cool to 45°C, centrifuge at 9690×g for 20min, wash with water to pH 6.5, 105 Dry at ℃ for 2h. The content of β-1,3-D-glucan in the obtained β-1,3-D-glucan product was 77.11%, and the extraction rate was 77.38%.

Embodiment 2

[0041] Add 12g of the cell wall of brewer’s yeast to 48mL of 0.8g / 100mL NaOH solution to form a cell wall suspension, treat it at 105°C for 5min, cool to 45°C, centrifuge at 9690×g for 20min, and wash with water to pH 7,80 Dry at ℃ for 4h. The content of β-1,3-D-glucan in the obtained β-1,3-D-glucan product is 76.95%, and the extraction rate is 78.31%.

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Abstract

The present invention discloses a method for extracting beta-1,3-D-glucan from the cell walls of fungi. The method comprises that cell walls and an alkali metal hydroxide are mixed in the presence of a solvent and an alkali treatment is performed, wherein the precipitate obtained from the alkali treatment is a beta-1,3-D-glucan crude product, the alkali metal hydroxide use amount is 65-900 g relative to per kg of the cell walls, and the alkali treatment temperature is 105-115 DEG C. With the technical scheme of the present invention, the beta-1,3-D-glucan extraction rate and the purity of the beta-1,3-D-glucan are high; the alkali consumption is low, such that the environmental pollution is less; and the time consumption is short, the extraction efficiency is high, and the unit energy consumption is less, such that the cost can be substantially reduced.

Description

Technical field [0001] The present invention belongs to the field of biotechnology, and relates to a method for preparing β-1,3-D-glucan, in particular to a method for extracting β-1,3-D-glucan from the cell wall of fungi . Background technique [0002] Saccharomyces cerevisiae (Saccharomyces cerevisiae) belongs to the genus Fungus Phylum. The cell wall is relatively tough, accounting for about 25% of the dry cell weight. The main components are glucan, mannan, protein, chitin and lipids. Among them, β-1,3-D-glucan, as a dietary fiber that is difficult to be digested by the human body, has important physiological functions in the body such as anti-cancer, anti-bacterial, anti-viral, lowering blood lipid and enhancing immune activity. Therefore, the development of β-1,3-D-glucan functional foods at home and abroad has become a hot spot. β-1,3-D-glucan has been popular as a medicine and health product in Japan, Southeast Asia, and Europe and the United States for more than ten yea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
Inventor 姜文侠杨萍田晓丽
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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