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Preparation method of hydrogel based on polyamide-amine dendritic molecule

A hydrogel and dendritic technology, applied in the field of polymer material preparation, can solve the problems of insufficient matching strength of hydrogels, lack of adhesion sites for hydrogels, cell agglomeration, etc. The effect of fast, safe and stable swelling rate in the combined process

Active Publication Date: 2016-06-08
PEKING UNIV
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  • Description
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Problems solved by technology

However, the strength of the hydrogel formed by these linear molecules is still not enough to match the strength required for tissue engineering.
In addition, the hydrogels formed by this class of molecules lack adhesion sites for cells, so when cells are present in these hydrogel scaffolds, they tend to aggregate and die

Method used

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  • Preparation method of hydrogel based on polyamide-amine dendritic molecule
  • Preparation method of hydrogel based on polyamide-amine dendritic molecule
  • Preparation method of hydrogel based on polyamide-amine dendritic molecule

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preparation example Construction

[0042] According to one aspect of the present invention, a kind of preparation method of the hydrogel based on polyamide-amine dendrimers is provided, the method comprises the following two steps:

[0043] (1) Add the first hydrogel factor and / or the second hydrogel factor into water and / or phosphate buffer solution, and mix well.

[0044] In the above preparation of the hydrogel, the reaction temperature is not particularly limited, in particular, the reaction is selected at room temperature.

[0045] In the above preparation of the hydrogel, the first hydrogel factor is O-PEG-DOPA, and the O-PEG-DOPA is shown in the following formula:

[0046]

[0047] In the above formula I, the corrugated line connected with the disulfide bond represents polyethylene glycol, and a long chain formed by polyethylene glycol can be called an arm, wherein, in the formula I, there are eight arms.

[0048] In the above formula I, the molecular weight of the first hydrogel factor is 12500-5260...

Embodiment 1

[0169] The preparation process of the first hydrogel factor O-PEG-DOPA

[0170] 1, Preparation: add 2.00g of dithiobipyridine and 426.36mg of mercaptopropanol into the methanol solution and mix, then add 1mL of acetic acid, stir at room temperature for 12h, and obtain the reaction system,

[0171] The reaction system was purified by column chromatography, wherein the eluent was dichloromethane:ethyl acetate=40:1;

[0172] 2) Preparation of:

[0173] Under ice-salt bath conditions, 340 mg of the purified product obtained in step 1) and 445.01 mg of p-nitrophenyl chloroformate were added to tetrahydrofuran and mixed, then 400 mg of triethylamine was added and reacted for 48 hours to obtain reaction system,

[0174] The above reaction system was purified by column chromatography, wherein the eluent: dichloromethane: petroleum ether = 20:1.

[0175] 3) Preparation of the first hydrogel factor O-PEG-DOPA:

[0176] Under the condition of avoiding light and normal temperat...

Embodiment 2

[0179] Preparation of Dendrimer Derivatives Containing RGDyC Polypeptide Group

[0180] 1) At room temperature, 25.03 mg of the fourth-generation polyamide-amine dendrimers and 187.73 mg of MAL-PEG-NHS were added to water and reacted for 15 minutes;

[0181] 2) Add 75.09 mgmPEG-NHS to the system obtained in step 1), and continue the reaction for 30 minutes;

[0182] 3) Add 13.73 mg of RGDyC to the system in step 2), and react for 2 hours to obtain a reaction system containing hydrogel factor (G-4.0-PEG-RGDyC),

[0183] The obtained reaction system was dialyzed with a dialysis bag with a molecular weight of 8000 for 48 hours, the liquid was changed every 8 hours, and then freeze-dried to obtain hydrogel factor, namely a dendrimer derivative containing RGDyC polypeptide group.

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Abstract

The present invention discloses a preparation method of a hydrogel based on a polyamide-amine dendritic molecule, the method is as follows: a first hydrogel factor and a second hydrogel factor are added into water and / or a phosphate buffer solution, after even mixing, an oxidizing agent is added to obtain a high strength good-biocompatibility degradable hydrogel, wherein the first hydrogel factor is obtained by modifying 8-arm polyethylene glycol in which sulfydryl is modified by dopamine, and the second hydrogel factor is obtained on the basis of the polyamide-amine dendritic molecule. The hydrogel can be applied in tissue engineering such as cartilage repair. The hydrogel prepared by the preparation method has stable swelling ratio, controllable degradation rate and strong mechanical properties, and the preparation method is simple and convenient and is easy to control, the whole cross-linking process is quick and safe, and production operation control and promotion can be facilitated.

Description

technical field [0001] The present invention relates to the field of polymer material preparation, in particular to a method for preparing hydrogels, in particular to a method for preparing hydrogels based on polyamide-amine dendrimers, which are used in tissue engineering, such as Application in repairing cartilage. Background technique [0002] Hydrogel is a material that can maintain a fluid state under certain conditions but can form a certain shape and strength under external physical or chemical stimuli. Hydrogel has a hydrophilic polymer network, which is close to the normal physiological environment of cells. Therefore, the use of hydrogel to prepare scaffold materials has unique advantages in repairing defects with complex shapes and minimally invasive treatment. An ideal hydrogel scaffold material needs to have the characteristics of easy cross-linking, low swelling, degradability, high strength and good biocompatibility. In recent years, hydrogels and their comp...

Claims

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Application Information

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IPC IPC(8): C08G83/00C08J3/075A61L27/52A61L27/22
Inventor 王尧贾欣茹
Owner PEKING UNIV
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