Rapid liquid submerged fermentation method for producing paecilomyces cicadae powder

Abstract

The invention discloses a rapid liquid submerged fermentation method for producing paecilomyces cicadae powder. According to the method, paecilomyces cicadae strains are adopted, then a semi-continuous fermentation technology is used to rapidly produce fermentation broth containing a large amount of mycelia, and finally the fermentation broth is separated by a micro filtration membrane to obtain mycelia of paecilomyces cicadae. The conventional paecilomyces cicadae intermittent batch fermentation technology is changed, and at the same time, the mycelia are separated by a high efficient ceramic micro-filtration membrane to replace the conventional centrifugal separation. The shortages of conventional batch fermentation technology such as a lot of steps, long time period between steps, low equipment efficiency, and the like are overcome by semi-continuous fermentation technology. Moreover, a high efficient ceramic membrane is used to filter fermentation broth to reduce the mycelia that enter the filtrate, the yield of mycelia is improved, the COD of filtrate is reduced, thus the processing amount of sewage is reduced, the cells are not obstructed, and effective components in cells can be completely recovered therefore. Compared with the conventional method, the provided method can generate prominent economic benefit, social benefit, and environmental benefit.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for rapidly producing fungal mycelium by deep liquid fermentation technology, more precisely, a method for rapidly producing Paecilomyces cicadae powder by deep liquid fermentation. Background technique [0002] Microbial fermentation can be divided into batch, fed-batch, semi-continuous, continuous and other modes. Batch fermentation consumes a lot of manpower and material resources. Each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. The process is cumbersome, the fermentation cycle is long, and the production efficiency is low; Fed-batch fermentation Although the lack of nutrients or precursors can be supplemented by feeding, the product synthesis will inevitably be blocked due to the continuous accumulation of harmful metabolites; the production intensity of continuous fermentation is greatly improved com...

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Problems solved by technology

[0006] The traditional Paecilomyces cicadae liquid fermentation process is mostly an intermittent batch fermentation process, but the batch fermentation process consumes a lot of manpower and material resources, and each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, cleaned, etc. The operation is complicated, the process is cumbersome, the non-fermentation period is long, and the production efficiency is low; while the subsequent separation process is traditionally separated by a centrifuge. This method is subject to the influence of the separation factor of the centrifuge, and the mycelium is subjected to shear force during the separation process. Destruction, part of the active ingredients are lost into the filtrate, resulting in a decrease in product quality and yield. The separated filtrate is turbid and contains more broken mycelia, resulting in a high COD of the discharged filtrate. If the filtrate meets the sewage discharge standard, it needs to pass through Complex sewage treatment, the workload of the sewage treatment system is too large, which brings a serious economic burden to the enterprise

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