A method for oxalic acid to promote bacterial leaching of photocatalytic semiconductor sulfide minerals
A semiconductor and photocatalytic technology, applied in the direction of using microorganisms, improving process efficiency, etc., to achieve the effects of easy control of conditions, mild conditions, and improved bacterial leaching efficiency
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example 1
[0019] The method described in this embodiment mainly proceeds according to the following steps:
[0020] (1) Inoculate 10% inoculum of Thiobacillus ferrooxidans into 100ml of 250ml shaker flask containing 1% chalcopyrite 9K medium for the first acclimatization, and use hemocytometer regularly every day count the number of living cells, when the bacterial concentration can reach 10 8 -10 9 individual / ml, complete the first domestication, remove the filter residue, and collect the bacteria by centrifugation, and add the collected bacterial solution to 2% chalcopyrite slurry for domestication until acidophilic Thiobacillus ferrooxidans can tolerate 2% chalcopyrite slurry Copper ore slurry concentration;
[0021] (2) The domesticated acidophilic Thiobacillus ferrooxidans was cultured in shake flasks in 9K medium containing 2% chalcopyrite, the culture conditions were initial pH 2.0, temperature 30°C, shaker speed 170 rpm;
[0022] (3) Filter and centrifuge the acidophilic Thio...
example 2
[0027] The method described in this embodiment mainly proceeds according to the following steps:
[0028] (1) Inoculate 10% inoculum of Thiobacillus ferrooxidans into 100ml of 250ml shaker flask containing 1% chalcopyrite 9K medium for the first acclimatization, and use hemocytometer regularly every day count the number of living cells, when the bacterial concentration can reach 10 9 individual / ml, complete the first domestication, remove the filter residue, and collect the bacteria by centrifugation, and add the collected bacterial solution to 2% chalcopyrite slurry for domestication until acidophilic Thiobacillus ferrooxidans can tolerate 2% chalcopyrite slurry Copper ore pulp concentration;
[0029] (2) The domesticated acidophilic Thiobacillus ferrooxidans was cultured in shake flasks in 9K medium containing 2% chalcopyrite, the culture conditions were initial pH 2.0, temperature 30°C, shaker speed 170 rpm;
[0030] (3) Filter and centrifuge the acidophilic Thiobacillus ...
example 3
[0035] The method described in this embodiment mainly proceeds according to the following steps:
[0036] (1) Inoculate 10% inoculum of Thiobacillus ferrooxidans into 100ml of 250ml shaker flask containing 1% chalcopyrite 9K medium for the first acclimatization, and use hemocytometer regularly every day count the number of living cells, when the bacterial concentration can reach 10 9 individual / ml, complete the first domestication, remove the filter residue, and collect the bacteria by centrifugation, and add the collected bacterial solution to 2% chalcopyrite slurry for domestication until acidophilic Thiobacillus ferrooxidans can tolerate 2% chalcopyrite slurry Copper ore slurry concentration;
[0037] (2) The domesticated acidophilic Thiobacillus ferrooxidans was cultured in shake flasks in 9K medium containing 2% chalcopyrite, the culture conditions were initial pH 2.0, temperature 30°C, shaker speed 170 rpm;
[0038] (3) Filter and centrifuge the acidophilic Thiobacillus ...
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