Desorption device for saxitoxin in macroporous resin and method of desorption device

A technology for shellfish toxin and pore adsorption, which is applied in the field of desorption of shellfish toxin and shellfish toxin desorption device in macroporous adsorption resin. Achieve the effect of avoiding residue and ensuring consistency

Active Publication Date: 2016-06-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method solves the time-consuming problem of the static desorption method, the steps of this method are cumbersome, and it is not convenient to process multiple samples at the same time. The moisture in the column is dried by positive pressure, which cannot completely blow the moisture Dry, resulting in subsequent steps such as dichloromethane extraction, centrifugation, etc.
In addition, the current macroporous adsorption resin desorption methods require a large amount of desorption liquid, so that it must be concentrated, redissolved, and filtered through nitrogen blowing or vacuum drying before it can be used for subsequent analysis

Method used

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  • Desorption device for saxitoxin in macroporous resin and method of desorption device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The target shellfish toxins in this embodiment are mainly fat-soluble shellfish toxins, such as OA, DTXs, PTXs, GYM, etc. Put the resin bag containing 3g of macroporous adsorption resin HP20 into the seawater 5 meters deep from the sea surface to absorb the fat-soluble shellfish toxin in the seawater, and take the resin bag back after 10 days. Put 12 resin bags in 500mL ultra-pure water, soak them 3 times, 5min each time, to remove salt and impurities; transfer the soaked macroporous adsorption resins to be desorbed into 30mL glass syringes 5 to form The macroporous adsorption resin layer 6 to be desorbed; open the vacuum pump 1, adjust the vacuum control valve 10, and carry out suction filtration until even if the vacuum degree inside the 12-hole solid-phase extraction instrument 9 is increased, the macroporous adsorption resin layer 6 to be desorbed Until no water flows out; close the vacuum pump 1, place the sample bottle 11 on the support in the solid-phase extracti...

Embodiment 2

[0030]The target shellfish toxin in this example is domoic acid toxin (DA). Put the resin bag containing 8g of macroporous adsorption resin SP207 into the seawater at a depth of 7 meters from the sea surface to adsorb DA toxin in the seawater, and take the resin bag back after 7 days. Place the resin bag in 1000mL ultrapure water, soak it twice, each time for 10min, to remove salt and impurities; transfer the soaked macroporous adsorption resin to be desorbed to a 50mL glass syringe 5 to form a desorbed Macroporous adsorption resin layer 6; open the vacuum pump 1, adjust the vacuum control valve 10, and perform suction filtration until even if the vacuum inside the solid phase extraction instrument 9 is increased, the macroporous adsorption resin layer 6 to be desorbed does not flow out; Turn off the vacuum pump 1, place the sample bottle 11 on the support in the solid phase extraction instrument 9, adjust the height of the support so that the guide needle 7 extends into the i...

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Abstract

The invention relates to a desorption device for saxitoxin in macroporous resin and a method of the desorption device. The desorption device comprises a vacuum pump and a solid-phase extraction instrument which are connected to the two ends of a transition bottle. A small column joint of the solid phase extraction instrument is connected with a microporus filter. A glass syringe is installed on the microporus filter. A filtering material layer is arranged at the bottom of the glass syringe. By means of the filtering material layer, macroporous resin can be effectively prevented from blocking the syringe. The method mainly includes the steps of flushing, moisture filtering, stirring, desorption, desorption solution collection and the like, filtering is completed at the same time during desorption, operation is easy, convenient and rapid, the time consumption is low, the steps of filtration and concentration can be omitted, and the use amount of a desorption solution can be effectively decreased.

Description

technical field [0001] The invention belongs to the technical field of monitoring shellfish toxins in water bodies, in particular to a desorption device for shellfish toxins in a macroporous adsorption resin and a method for using the device to desorb shellfish toxins. Background technique [0002] In recent years, due to the massive discharge of domestic sewage and industrial wastewater, the impact of agriculture, aquaculture and human activities, the eutrophication of coastal waters has become increasingly serious, coupled with the impact of global climate change, harmful red tides in estuaries, oceans and even freshwater lakes Outbreaks are becoming more and more frequent, and shellfish toxin pollution incidents have become a problem that needs to be solved urgently worldwide. The shellfish toxins are mainly poisonous and harmful algae toxins secreted by red tide algae. Since they are usually detected in shellfish, these biological toxins are collectively referred to as s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 佟蒙蒙渠佩佩
Owner ZHEJIANG UNIV
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