Compositions and methods for biodegrading plastic
A composition, technology of laccase, applied in the field of composition based on thermophilic laccase, capable of solving problems like no other
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Embodiment 1
[0112] Biodegradation of polyethylene by newly isolated laccase from soil bacteria
[0113] This research is devoted to the induction and optimization of the activity of bacterial laccase, which is found to be related to the biodegradation of plastics.
[0114] It has been found that two soil bacteria show favorable polyethylene biodegradability. They were sequenced based on the 16SrRNA genes of the two bacteria, and they were identified as Bacillus potsdam and Bacillus agrobacterium. The 16SrRNA gene is shown in Picture 11 in. Picture 12 The phylogenetic tree of two strains based on their 16SrRNA sequence with Rhodococcus erythropolis as a foreign strain is shown.
[0115] The characteristics of the two strains indicate that they are thermophilic. The optimal growth temperature of Potsdam Brevibacterium is 40℃, and the optimal growth temperature of Agrobacterium Brevibacterium is 45℃ ( figure 1 ). In addition, it was also found that the optimal pH growth conditions were pH=6.0 ...
Embodiment 2
[0122] Secretion of extracellular laccase
[0123] In the presence of 2,6-dimethoxyphenol (DMP), the secretion of extracellular laccase was tested, and it was found that the laccase was related to the biodegradation process. The oxidation of this substrate of the enzyme causes the color of the solution to turn brown. After incubating the extracellular enzyme with 30mMDMP for 19 hours at room temperature, compared with the blank ( Figure 14 ) To get brown ( Figure 15 ). This indicates that laccase is secreted into the medium outside the cell.
Embodiment 3
[0125] The effect of different additives on laccase activity
[0126] In order to improve the enzyme activity, the effects of different additives added to the bacterial growth medium were examined. Test the effect on the induction and activity of two laccases. Check the following additives: copper (Cu 2+ ), ABTS, xylan, the synergistic effect of copper and xylan, cobalt and nickel.
[0127] Evaluation of Cu added to the growth medium 2+ Effect on the induction and activity of laccase in two kinds of bacteria. In Brevibacterium potsdam, it was found that the addition of Cu2+ at an increased concentration resulted in lower induction of laccase, but higher specific activity ( Figure 4 with 5 ).
[0128] In Bacillus agrobacterium, it has been found that adding an increased concentration of Cu2+ improves the induction and specific activity of laccase ( Image 6 ). In addition, monitor the effect of Cu concentration on the concentration of laccase in Bacillus brevis ( Figure 7 ). Th...
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