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Hemostatic material, preparation method and application thereof

A hemostatic material and biodegradable technology, applied in the field of biomedical hemostatic materials, can solve the problems of poor adhesion of visceral wound sites, limited hemostatic effect, increased risk of wound site infection, etc., and achieve good biocompatibility and degradability. Effect

Active Publication Date: 2016-06-15
RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although fibrin glue has good adhesion, it cannot be used alone for hemostasis on large wounds because it cannot be compressed to stop bleeding. In addition, it is easy to cause allergies and viral infections because it is derived from the blood of animals and humans; The structure makes it have a strong absorption effect on blood, activates platelets, and promotes thrombus formation, but its adhesion to visceral trauma sites is poor, and its degradation and absorption in the body are poor, which greatly increases the risk of infection at the wound site ; Although chitosan has a certain antibacterial and hemostatic effect, the hemostatic effect on larger bleeding sites is not ideal due to the limited hemostatic effect

Method used

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preparation example Construction

[0029] The present invention also provides a method for preparing the above hemostatic material, which includes the following steps:

[0030] A biodegradable polypeptide is prepared from an amino acid containing a protective group and phenylboronic acid or its pinacol ester. The biodegradable polypeptide is sequentially composed of phenylboronic acid, ion self-complementary polypeptide segment, and cell adhesion promoting polypeptide from the nitrogen end to the carbon end. segment.

[0031] To the solution containing the biodegradable polypeptide, the viscosity enhancer is added and mixed uniformly to obtain a hemostatic material.

[0032] 1. Prepare a biodegradable polypeptide with an amino acid containing a protective group and phenylboronic acid or its pinacol ester. The biodegradable polypeptide is composed of phenylboronic acid, ionic self-complementary polypeptide segment, and cell adhesion promotion. With peptide section.

[0033] The preparation of the biodegradable polypept...

Embodiment 1

[0060] (1) Preparation of biodegradable polypeptide B(OH) 2 FKFEFKFEFKFEGRGDS-OH(P-I).

[0061] Take 1.0 g of 2-chloro-trityl chloride resin (degree of substitution 1.08 mmol / g, 1% DVB) in a solid phase synthesis column, swell with 8 mL of DMF for 1 hour, and drain the DMF solvent. Take 10 mL of DMF solution containing 0.77 g of FMOC-Ser(tBu)-OH and add it to the swollen resin, continue to add 1.3 mL of DIEA, stir and react for 1.5 hours, drain the reaction solution, and wash with 10 mL of DMF four times.

[0062] Take 8mL of 20% Piperidine / DMF solution into the above reaction column, react for 20 minutes, remove the FMOC protecting group at the amino terminal, wash with 10mL DMF four times, continue to add 12mL containing 0.82g FMOC-Asp(OtBu) -OH, 0.91gHBTU, 0.32gHOBt and 0.65mL DIEA in DMF solution, stirred and reacted for 2 hours, drained the solvent, and washed four times with 10mL DMF. Add 1 mL of 10 mg / mL ninhydrin / methanol solution to the above-mentioned small amount of res...

Embodiment 2

[0069] (1) Preparation of biodegradable polypeptide B(OH) 2 FKFEFKFEFKFEGRGDS-OH (P-I), as shown in Example 1.

[0070] (2) Preparation of biodegradable polypeptide solution.

[0071] The polypeptide P-I is dissolved in a physiological saline solution with a pH of 7.4 to prepare a solution with a concentration of 2.4% by mass, dispersed by ultrasound, and allowed to stand for 2 hours at room temperature to obtain a solution of the biodegradable polypeptide.

[0072] (3) Preparation of peptide hemostatic material cross-linked by adhesion protein.

[0073] Dissolve the adhesion protein Mefp-3 in a physiological saline solution with a pH of 7.4 to prepare a solution with a mass percentage concentration of 1.2%. Mix the Mefp-3 solution with the biodegradable polypeptide solution in (2) in equal volumes, and vortex In 1 min, the peptide hemostatic material cross-linked by adhesion protein is obtained.

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PUM

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Abstract

A hemostatic material includes 2-100 parts of a biodegradable polypeptide, 1-60 parts of an adhesion enhancer, and 840-997 parts of water. The biodegradable polypeptide is crosslinked with the adhesion enhancer. The invention also provides a preparation method and an application of the hemostatic material. The hemostatic material has excellent biocompatibility and biodegradability, is independent from clotting mechanism of body, and has excellent hemostatic effect and promotes wound tissue repair.

Description

Technical field [0001] The invention relates to the field of biomedical hemostatic materials, in particular to a hemostatic material and a preparation method and application thereof. Background technique [0002] Loss of control of bleeding is the main cause of many sudden accidents, such as traffic accidents, natural disasters such as typhoons, earthquakes, tsunamis, mudslides, and major medical operations, causing deaths or deaths from battle injuries. The effective and rapid hemostasis on the traumatic site can be achieved. Improve the chances of treatment for the injured and turn around critical lives. Therefore, it is of great significance to develop new medical absorbable hemostatic materials that are fast, safe, and effective for on-site, pre-hospital and in-surgery emergency use. [0003] With the rapid development of science and technology, hemostatic materials have been greatly developed. At present, the common hemostatic materials in the domestic market mainly include ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/00A61L24/12
CPCA61L24/0015A61L24/0042A61L24/108A61L24/12A61L2300/252A61L2300/412A61L2400/04
Inventor 陈昌盛李小丽刘伟强佘振定
Owner RESEARCH INSTITUTE OF TSINGHUA UNIVERSITY IN SHENZHEN
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