Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of candida strain and its application, and the method for processing Pu'er tea

A technology of Candida and Pu'er tea, applied in the biological field, achieves the effects of obvious sweetness, shortening the fermentation cycle and optimizing fermentation conditions

Active Publication Date: 2019-05-10
MENGHAI TEA IND
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the use of Candida blankii in the fermentation and processing of Pu-erh tea

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of candida strain and its application, and the method for processing Pu'er tea
  • A kind of candida strain and its application, and the method for processing Pu'er tea
  • A kind of candida strain and its application, and the method for processing Pu'er tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: Separation and purification of Candida TMCC 70011 strain

[0057] Collect fresh fermented tea samples from the fermenting Pu'er tea pile (collected from Menghai Tea Industry Co., Ltd.), and obtain 150 strains of Candida Blanche by isolation of the purification medium described below. After morphological observation and gene sequencing (entrusted to Shenzhen Huada Gene Company) analysis, the TMCC 70011 strain was selected.

[0058] The separation and purification method is as follows:

[0059] (1) Purification medium preparation:

[0060] Medium formula: peptone 4g, glucose 4g, yeast extract 5g, agarose 2g, distilled water 1000ml, pH is natural state (6.8-7.3).

[0061] Sterilize the prepared medium at 121°C for 40 minutes, cool to about 45°C, pour it into sterile petri dishes, about 20ml per dish, cool and solidify for later use;

[0062] (2) Sample treatment: take the Pu-erh tea fermented pile tea sample to be separated, grind and pulverize, take 1g, a...

Embodiment 2

[0067] Embodiment 2: the production application of Candida TMCC 70011 bacterial strain

[0068] The purified TMCC 70011 strain was inoculated into ISP No. 2 liquid medium by picking a single colony from the plate, and cultured in a shaker at 150 rpm and 30°C for 7 hours until the growth index phase. Add water to the large-leaf sun-dried green tea (collected from Menghai Tea Industry Co., Ltd.) until the water content reaches 35% (m / m) based on the total weight of the sun-dried green tea before the tide, and sterilize at 121°C for 30 minutes. Sun-dried green hair tea 1.0×10 per gram 5 The TMCC 70011 bacterial strain cultivated to the growth exponential phase was inoculated with an inoculum amount of 1, and then placed at 35°C for 3 weeks. Replenish water and turn the heap on a weekly basis, and replenish water according to the amount of 10% (v / m) (based on the total weight of the culture before each water replenishment) each time. With the mass reproduction of the TMCC 70011 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to a candida blankii strain and application thereof as well as a method for processing Pu'er tea. The preservation number of the candida blankii is CGMCC No.8682. The strain is applicable to the processing of the Pu'er tea. The method for processing the Pu'er tea comprises steps of inoculating candida blankii obtained from separation and purification to Dayezhong sun-dried crude tea, and conducting fermentation cultivation. According to the method disclosed by the invention, the intrinsic quality of the Pu'er tea is reserved, and moreover the special fragrance and bright soup color of the Pu'er tea are enhanced, so that obtained cooked Pu'er tea has dark red and slightly turbid soup color, significant sweetness and has the characteristics of being remarkable and durable in fragrance as sophora flower; and moreover, a fermentation cycle is shortened.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a Candida strain and application thereof, and a method for processing Pu'er tea. Background technique [0002] With the development of modern biotechnology, yeast has been widely used in many aspects of people's lives, such as brewing wine, fermenting fruit juice, fermenting bread, making dairy products, and making delicious food. Yeast has a natural and rich nutritional system, and it is a pure natural, pollution-free, biological source of healthy nutrition. Yeast is hailed as the most attractive nutrient by the nutritional circles. According to reports, yeast contains more than 50 kinds of nutritional components, and proteins, vitamin B groups, minerals, nucleic acids, etc. necessary for human health can all be obtained from yeast. [0003] In the traditional Pu-erh tea stacking fermentation, the combined effects of microorganisms, heat and humidity, oxidation and enz...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16A23F3/10C12R1/72
Inventor 高林瑞唐蜀昆刘韬卢开阳任万增童一峰黄华伟田飞邹小林卢晓慧
Owner MENGHAI TEA IND