Determination method of sucrose utilization rate in tissue culture seedlings

A determination method and technology for tissue culture seedlings, applied in the field of plant biology, can solve the problems that the autotrophic share and heterotrophic share contributed by tissue culture seedlings cannot be determined, and the autotrophic ability of tissue culture seedlings cannot be represented, etc. Achieving the effect of few steps and simple calculation

Active Publication Date: 2018-09-21
INST OF GEOCHEM CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0004] Therefore, the determination of the autotrophic portion of the tissue culture seedlings and the determination of the sucrose utilization rate are particularly important. Although Li-840 CO 2 / H 2 CO established by the O analyzer 2 The exchange rate measurement system is used to measure the self-supporting ability of tissue cultured seedlings, but the test is only the instantaneous self-supporting ability, which cannot represent the situation of the self-supporting ability of tissue-cultured seedlings within a period of time, let alone measure the self-supporting ability of tissue-cultured seedlings within a period of time. The trophic and heterotrophic shares and the contribution from tissue culture plantlet respiration

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  • Determination method of sucrose utilization rate in tissue culture seedlings
  • Determination method of sucrose utilization rate in tissue culture seedlings
  • Determination method of sucrose utilization rate in tissue culture seedlings

Examples

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Effect test

Embodiment 1

[0048] Sucrose Utilization Efficiency of Brassica napus Tissue Culture Seedlings under Different Nitrogen Concentrations in Example 1

[0049] According to the method of the present invention, the brassica napus tissue culture seedlings are cultured under the conditions of low nitrogen, medium nitrogen, control and high nitrogen respectively, and sugar cane sucrose and beet sucrose are respectively used as carbon sources. The δ of the new leaves 13 C values ​​are shown in Table 1.

[0050] Table 1 New leaf δ of tissue cultured seedlings 13 C value

[0051]

[0052] According to table 1, the δ of new leaves of tissue cultured seedlings 13 C value, using equation (3) and equation (4), just can calculate the various proportions of tissue culture seedlings, each proportion of tissue culture seedlings is shown in Table 2.

[0053] Table 2 Various shares of tissue culture seedlings

[0054]

[0055] It can be seen from Table 2 that under the nitrogen concentration gradien...

Embodiment 2

[0065] Example 2 The nitrate nitrogen concentration is constant, and the sucrose utilization rate of Brassica napus tissue culture seedlings is treated by increasing the ammonium nitrogen concentration successively

[0066]According to the method of the present invention, the Brassica napus tissue culture seedlings are respectively under the culture condition that ammonium nitrogen concentration is 7mM, 14mM and 28mM, each corresponding ammonium attitude concentration treatment all adds the nitrate nitrogen of 39mM respectively, uses respectively Cane sucrose and beet sucrose were used as carbon sources. The δ of the new leaves 13 C values ​​are shown in Table 5.

[0067] Table 5 New leaf δ of tissue cultured seedlings 13 C value

[0068]

[0069]

[0070] According to table 5, the δ of the new leaves of tissue cultured seedlings 13 C value, using equation (3) and equation (4), just can calculate the various proportions of tissue culture seedlings, each proportion of...

Embodiment 3

[0082] Example 3 The concentration of ammonium nitrogen is constant, and the sucrose utilization rate of Brassica napus tissue culture seedlings is treated by increasing the concentration of nitrate nitrogen in turn

[0083] According to the method of the present invention, the Brassica napus tissue culture seedlings are respectively under the culture condition that nitrate nitrogen concentration is 13mM, 26mM and 53mM, each corresponding nitrate concentration treatment all adds the ammonium nitrogen of 21mM respectively, uses respectively Cane sucrose and beet sucrose were used as carbon sources. The δ of the new leaves 13 C values ​​are shown in Table 9.

[0084] Table 9 New leaf δ of tissue cultured seedlings 13 C value

[0085]

[0086] According to the δ of the new leaves of the tissue cultured seedlings in Table 9 13 The value of C, using equation (3) and equation (4), can calculate the various proportions of the tissue cultured seedlings, and the various proporti...

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Abstract

The invention discloses a determining method of tissue cultured seedling sucrose. The method comprises the following steps of respectively adopting beet sucrose and sugarcane sucrose as organic carbon sources to prepare a beet sucrose culture medium and a sugarcane sucrose culture medium; culturing tissue cultured seedlings with consistent growth vigors until the fresh weight is increased by over 20 times, and finishing the culturing; determining delta<13>C values of the beet sucrose, the sugarcane sucrose and newly-growing leaves; according to the tissue cultured seedlings of plants to be determined, utilizing the sugarcane sucrose and the beet sucrose to produce carbon isotope fractionation values, and utilizing a fractionation value of carbon dioxide in air, the weight increment of the tissue cultured seedlings and sugarcane consumption to calculate the sugarcane utilization rate of the tissue cultured seedlings. The method provided by the invention not only can be used for quantitatively determining the autotrophy portion, the heterotrophy portion and the like of the tissue cultured seedlings in one growth period, but also can be used for calculating the sugarcane utilization rate of the tissue cultured seedlings in the same period, has an important guide value on regulation of tissue cultured environment factors, and provides technical parameters for domestication and transplantation of later tissue cultured seedlings.

Description

technical field [0001] The invention relates to a method for measuring the sucrose utilization rate of tissue cultured seedlings, and belongs to the field of plant biotechnology. technical background [0002] Plant tissue culture is the most basic technology and method in current biotechnology, and has been widely used in horticulture, agriculture and forestry production. It is a technology for rapid breeding of plants under certain conditions such as artificially provided temperature, light, humidity, nutrition, and hormones. [0003] In the process of plant tissue culture, there are three growth methods of tissue culture seedlings: one is the autotrophic growth of plantlets by photosynthesis; the other is heterotrophic growth of plantlets by the organic carbon source in the medium; the third is plantlet growth Relying on the organic carbon source in the medium and artificial light, both heterotrophic and autotrophic growth can be carried out at the same time. Most of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 吴沿友张开艳赵丽华饶森杭红涛李海涛
Owner INST OF GEOCHEM CHINESE ACADEMY OF SCI
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